Expression and Immune Response Analysis of Schistosoma Japonicum VAL-1, a Homologue of Vespid Venom Allergens

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2010 Mar 23

PMID 20306204

Citations 16

Authors

Jingfang Chen

Xuchu Hu

Sijie He

Lexun Wang

Dong Hu

Xiaoyun Wang

Minghui Zheng

Yabo Yang

Chi Liang

Jin Xu

Xinbing Yu

Affiliations

Soon will be listed here.

Abstract

Many parasites such as trematodes and nematodes have been found to express members of a gene family variously termed as venom allergen-like protein (VAL) or sperm-coating protein (SCP)-like protein. The molecular functions of these proteins remain unclear. We isolated the corresponding gene from Schistosoma japonicum, which we designated as Sj-VAL-1. The cDNA of Sj-VAL-1 contains an open reading frame encoding 221 amino acids, the first 25 residues being a putative secretion signal. RT-PCR analysis confirmed that Sj-VAL-1 was transcribed mainly in cercariae and eggs stages. Western blot analysis indicated that Sj-VAL-1 protein was an egg excretory-secretory products (ES products). Immunofluorescence showed it was secreted by eggs, head gland, and penetration glands of cercariae. In S. japonicum-infected mice, Sj-VAL-1-specific antibody significantly increased 6 weeks after infection and a higher level of IgG1 antibody contrast to IgG2a antibody indicated that a polarized Th2 immune response could be induced by Sj-VAL-1. These findings suggest that Sj-VAL-1 must play a role in the interaction between parasite and host. Its role as a potential modulator of immune function or as a pathogenic factor of granuloma formation in schistosomiasis needs further study.

Citing Articles

excretory/secretory products: an untapped library of tolerogenic immunotherapeutics against food allergy.

Rogers M, Kamath S, McManus D, Jones M, Gordon C, Navarro S Clin Transl Immunology. 2024; 13(9):e70001.

PMID: 39221178 PMC: 11359118. DOI: 10.1002/cti2.70001.

Excretory/Secretory Proteome of Females and Males of the Hookworm .

Uzoechi S, Rosa B, Singh K, Choi Y, Bracken B, Brindley P Pathogens. 2023; 12(1).

PMID: 36678443 PMC: 9865600. DOI: 10.3390/pathogens12010095.

Immune Evasion Strategies of Schistosomes.

Hambrook J, Hanington P Front Immunol. 2021; 11:624178.

PMID: 33613562 PMC: 7889519. DOI: 10.3389/fimmu.2020.624178.

Cross-reactive carbohydrate determinant-specific IgE obscures true atopy and exhibits ⍺-1,3-fucose epitope-specific inverse associations with asthma.

Nkurunungi G, Mpairwe H, Versteeg S, van Diepen A, Nassuuna J, Kabagenyi J Allergy. 2020; 76(1):233-246.

PMID: 32568414 PMC: 7610925. DOI: 10.1111/all.14469.

Schistosoma mansoni venom allergen-like proteins: phylogenetic relationships, stage-specific transcription and tissue localization as predictors of immunological cross-reactivity.

Farias L, Chalmers I, Perally S, Rofatto H, Jackson C, Brown M Int J Parasitol. 2019; 49(8):593-599.

PMID: 31136745 PMC: 6598858. DOI: 10.1016/j.ijpara.2019.03.003.

References

Abdel-Hafeez E, Kikuchi M, Watanabe K, Ito T, Yu C, Chen H . Proteome approach for identification of schistosomiasis japonica vaccine candidate antigen. Parasitol Int. 2008; 58(1):36-44. DOI: 10.1016/j.parint.2008.09.004. View

Engels D, Chitsulo L, Montresor A, Savioli L . The global epidemiological situation of schistosomiasis and new approaches to control and research. Acta Trop. 2002; 82(2):139-46. PMC: 5633073. DOI: 10.1016/s0001-706x(02)00045-1. View

Fitzpatrick J, Johnston D, Williams G, Williams D, Freeman T, Dunne D . An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression. Mol Biochem Parasitol. 2005; 141(1):1-13. DOI: 10.1016/j.molbiopara.2005.01.007. View

Hawdon J, Narasimhan S, Hotez P . Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum. Mol Biochem Parasitol. 1999; 99(2):149-65. DOI: 10.1016/s0166-6851(99)00011-0. View

Schramm G, Hamilton J, Balog C, Wuhrer M, Gronow A, Beckmann S . Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Mol Biochem Parasitol. 2009; 166(1):4-14. DOI: 10.1016/j.molbiopara.2009.02.003. View

Murphy E, Zhang Y, Zhu W, Biggs J . The human glioma pathogenesis-related protein is structurally related to plant pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene. 1995; 159(1):131-5. DOI: 10.1016/0378-1119(95)00061-a. View

Lu G, Villalba M, Coscia M, Hoffman D, King T . Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets. J Immunol. 1993; 150(7):2823-30. View

Milne T, Abbenante G, Tyndall J, Halliday J, Lewis R . Isolation and characterization of a cone snail protease with homology to CRISP proteins of the pathogenesis-related protein superfamily. J Biol Chem. 2003; 278(33):31105-10. DOI: 10.1074/jbc.M304843200. View

Anand S, Gnanasekar M, Thangadurai M, Prabhu P, Kaliraj P, Ramaswamy K . Immune response studies with Wuchereria bancrofti vespid allergen homologue (WbVAH) in human lymphatic filariasis. Parasitol Res. 2007; 101(4):981-8. PMC: 2763210. DOI: 10.1007/s00436-007-0571-2. View

10.

Pearce E . Priming of the immune response by schistosome eggs. Parasite Immunol. 2005; 27(7-8):265-70. DOI: 10.1111/j.1365-3024.2005.00765.x. View

11.

Yatsuda A, Eysker M, Vieira-Bressan M, de Vries E . A family of activation associated secreted protein (ASP) homologues of Cooperia punctata. Res Vet Sci. 2002; 73(3):297-306. DOI: 10.1016/s0034-5288(02)00125-x. View

12.

Pearce E, MacDonald A . The immunobiology of schistosomiasis. Nat Rev Immunol. 2002; 2(7):499-511. DOI: 10.1038/nri843. View

13.

Visser A, Van Zeveren A, Meyvis Y, Peelaers I, Van den Broeck W, Gevaert K . Gender-enriched transcription of activation associated secreted proteins in Ostertagia ostertagi. Int J Parasitol. 2007; 38(3-4):455-65. DOI: 10.1016/j.ijpara.2007.08.008. View

14.

Hawdon J, Jones B, Perregaux M, Hotez P . Ancylostoma caninum: metalloprotease release coincides with activation of infective larvae in vitro. Exp Parasitol. 1995; 80(2):205-11. DOI: 10.1006/expr.1995.1025. View

15.

Kovalick G, Griffin D . Characterization of the SCP/TAPS gene family in Drosophila melanogaster. Insect Biochem Mol Biol. 2005; 35(8):825-35. DOI: 10.1016/j.ibmb.2005.03.003. View

16.

Hawdon J, Jones B, Hoffman D, Hotez P . Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae. J Biol Chem. 1996; 271(12):6672-8. DOI: 10.1074/jbc.271.12.6672. View

17.

Reinke V, Gil I, Ward S, Kazmer K . Genome-wide germline-enriched and sex-biased expression profiles in Caenorhabditis elegans. Development. 2003; 131(2):311-23. DOI: 10.1242/dev.00914. View

18.

Hu W, Yan Q, Shen D, Liu F, Zhu Z, Song H . Evolutionary and biomedical implications of a Schistosoma japonicum complementary DNA resource. Nat Genet. 2003; 35(2):139-47. DOI: 10.1038/ng1236. View

19.

Boucheix C, Rubinstein E . Tetraspanins. Cell Mol Life Sci. 2001; 58(9):1189-205. PMC: 11337403. DOI: 10.1007/PL00000933. View

20.

Chalmers I, McArdle A, Coulson R, Wagner M, Schmid R, Hirai H . Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family. BMC Genomics. 2008; 9:89. PMC: 2270263. DOI: 10.1186/1471-2164-9-89. View