Fine Structure Genetic and Physical Map of the Gene 3 to 10 Region of the Bacteriophage P22 Chromosome

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1991 Apr 1

PMID 2029965

Citations 4

Authors

S Casjens

K Eppler

L Sampson

R Parr

E Wyckoff

Affiliations

Soon will be listed here.

Abstract

The mechanism by which dsDNA is packaged by viruses is not yet understood in any system. Bacteriophage P22 has been a productive system in which to study the molecular genetics of virus particle assembly and DNA packaging. Only five phage encoded proteins, the products of genes 3, 2, 1, 8 and 5, are required for packaging the virus chromosome inside the coat protein shell. We report here the construction of a detailed genetic and physical map of these genes, the neighboring gene 4 and a portion of gene 10, in which 289 conditional lethal amber, opal, temperature sensitive and cold sensitive mutations are mapped into 44 small (several hundred base pair) intervals of known sequence. Knowledge of missense mutant phenotypes and information on the location of these mutations allows us to begin the assignment of partial protein functions to portions of these genes. The map and mapping strains will be of use in the further genetic dissection of the P22 DNA packaging and prohead assembly processes.

Citing Articles

'Let the phage do the work': using the phage P22 coat protein structures as a framework to understand its folding and assembly mutants.

Teschke C, Parent K Virology. 2010; 401(2):119-30.

PMID: 20236676 PMC: 2862144. DOI: 10.1016/j.virol.2010.02.017.

The pKO2 linear plasmid prophage of Klebsiella oxytoca.

Casjens S, Gilcrease E, Huang W, Bunny K, Pedulla M, Ford M J Bacteriol. 2004; 186(6):1818-32.

PMID: 14996813 PMC: 355964. DOI: 10.1128/JB.186.6.1818-1832.2004.

A P22 scaffold protein mutation increases the robustness of head assembly in the presence of excess portal protein.

Moore S, Prevelige Jr P J Virol. 2002; 76(20):10245-55.

PMID: 12239300 PMC: 136566. DOI: 10.1128/jvi.76.20.10245-10255.2002.

Genetic properties of temperature-sensitive folding mutants of the coat protein of phage P22.

Gordon C, King J Genetics. 1994; 136(2):427-38.

PMID: 8150274 PMC: 1205798. DOI: 10.1093/genetics/136.2.427.

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