Comparison of Nuclease Digestion of Polyoma Virus Nucleoprotein Complex and Mouse Chromatin

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1978 Jan 1

PMID 202734

Citations 6

Authors

B A Ponder

F Crew

L V CRAWFORD

Affiliations

Soon will be listed here.

Abstract

We digested polyoma virus nucleoprotein complex, isolated from disrupted virions, with micrococcal nuclease and DNase I. The results were compared with digestions of chromatin from mouse nuclei. The nucleosome "core" structures were similar, but the spacing of the nucleosomes in the isolated polymoma nucleoprotein complexes was irregular, whereas in mouse chromatin it was regular. The average nucleosome repeat length in each case was 190 to 200 base pairs. This figure suggests that, unless there are substantial stretches of free DNA, the polyoma nucleoprotein complex contains about 26 nucleosomes. The commonly used method of preparing the nucleoprotein complex by disruption of virions at pH 10.2 may lead to significant damage to the structure. Such damage may be more clearly revealed by the susceptibility of the DNA to nuclease digestion than by the usual criteria of sedimentation velocity and buoyant density.

Citing Articles

Polyomavirus DNA is damaged in target cells during cytotoxic T-lymphocyte-mediated killing.

Sellins K, Cohen J J Virol. 1989; 63(2):572-8.

PMID: 2536092 PMC: 247725. DOI: 10.1128/JVI.63.2.572-578.1989.

Structural changes in simian virus 40 chromatin as probed by restriction endonucleases.

Liggins G, English M, Goldstein D J Virol. 1979; 31(3):718-32.

PMID: 229248 PMC: 353500. DOI: 10.1128/JVI.31.3.718-732.1979.

Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells.

Fernandez-Munoz R, Coca-Prados M, Hsu M J Virol. 1979; 29(2):612-23.

PMID: 219250 PMC: 353194. DOI: 10.1128/JVI.29.2.612-623.1979.

Simian virus 40 DNA replication in isolated replicating viral chromosomes.

Su R, DePamphilis M J Virol. 1978; 28(1):53-65.

PMID: 212613 PMC: 354247. DOI: 10.1128/JVI.28.1.53-65.1978.

Chromosome of the mature virion of simian virus 40 contains H1 histone.

Nedospasov S, Bakayev V, Georgiev G Nucleic Acids Res. 1978; 5(8):2847-60.

PMID: 211487 PMC: 342211. DOI: 10.1093/nar/5.8.2847.

References

Christiansen G, Landers T, Griffith J, Berg P . Characterization of components released by alkali disruption of simian virus 40. J Virol. 1977; 21(3):1079-84. PMC: 515648. DOI: 10.1128/JVI.21.3.1079-1084.1977. View

Meinke W, Hall M, Goldstein D . Proteins in intracellular simian virus 40 nucleoportein complexes: comparison with simian virus 40 core proteins. J Virol. 1975; 15(3):439-48. PMC: 354478. DOI: 10.1128/JVI.15.3.439-448.1975. View

McCann P, Martin R . Nuclease digestion of the DNA-protein complex from simian virus 40 virions. Biochim Biophys Acta. 1975; 395(3):280-3. DOI: 10.1016/0005-2787(75)90198-7. View

Germond J, Hirt B, Oudet P, Chambon P . Folding of the DNA double helix in chromatin-like structures from simian virus 40. Proc Natl Acad Sci U S A. 1975; 72(5):1843-7. PMC: 432643. DOI: 10.1073/pnas.72.5.1843. View

Louie A . The organization of proteins in polyoma and cellular chromatin. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:259-66. DOI: 10.1101/sqb.1974.039.01.034. View

Polisky B, McCarthy B . Location of histones on simian virus 40 DNA. Proc Natl Acad Sci U S A. 1975; 72(8):2895-9. PMC: 432885. DOI: 10.1073/pnas.72.8.2895. View

Cremisi C, Pignatti P, CROISSANT O, Yaniv M . Chromatin-like structures in polyoma virus and simian virus 10 lytic cycle. J Virol. 1975; 17(1):204-11. PMC: 515404. DOI: 10.1128/JVI.17.1.204-211.1976. View

Keller W . Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis. Proc Natl Acad Sci U S A. 1975; 72(12):4876-80. PMC: 388835. DOI: 10.1073/pnas.72.12.4876. View

Schaffhausen B, BENJAMIN T . Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus. Proc Natl Acad Sci U S A. 1976; 73(4):1092-6. PMC: 430206. DOI: 10.1073/pnas.73.4.1092. View

10.

Varshavsky A, Bakayev V, Chumackov P, Georgiev G . Minichromosome of simian virus 40: presence of histone HI. Nucleic Acids Res. 1976; 3(8):2101-13. PMC: 343065. DOI: 10.1093/nar/3.8.2101. View

11.

BELLARD M, Oudet P, Germond J, Chambon P . Subunit structure of simian-virus-40 minichromosome. Eur J Biochem. 1976; 70(2):543-53. DOI: 10.1111/j.1432-1033.1976.tb11046.x. View

12.

Fried M, Griffin B . Organization of the genomes of polyoma virus and SV40. Adv Cancer Res. 1977; 24:67-113. DOI: 10.1016/s0065-230x(08)61013-1. View

13.

Ponder B, CRAWFORD L . The arrangement of nucleosomes in nucleoprotein complexes from polyoma virus and SV40. Cell. 1977; 11(1):35-49. DOI: 10.1016/0092-8674(77)90315-4. View

14.

Lohr D, Corden J, Tatchell K, Kovacic R, VAN Holde K . Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin. Proc Natl Acad Sci U S A. 1977; 74(1):79-83. PMC: 393200. DOI: 10.1073/pnas.74.1.79. View

15.

Morris N . Nucleosome structure in Aspergillus nidulans. Cell. 1976; 8(3):357-63. DOI: 10.1016/0092-8674(76)90147-1. View

16.

Noll M, Kornberg R . Action of micrococcal nuclease on chromatin and the location of histone H1. J Mol Biol. 1977; 109(3):393-404. DOI: 10.1016/s0022-2836(77)80019-3. View

17.

Camerini-Otero R, Felsenfeld G . The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel. Cell. 1976; 8(3):333-47. DOI: 10.1016/0092-8674(76)90145-8. View

18.

Morris N . A comparison of the structure of chicken erythrocyte and chicken liver chromatin. Cell. 1976; 9(4 Pt 1):627-32. DOI: 10.1016/0092-8674(76)90045-3. View

19.

Finch J, Noll M, Kornberg R . Electron microscopy of defined lengths of chromatin. Proc Natl Acad Sci U S A. 1975; 72(9):3320-2. PMC: 432983. DOI: 10.1073/pnas.72.9.3320. View

20.

Lacy E, Axel R . Analysis of DNA of isolated chromatin subunits. Proc Natl Acad Sci U S A. 1975; 72(10):3978-82. PMC: 433121. DOI: 10.1073/pnas.72.10.3978. View