Expression of MAP3 Kinase COT1 is Up-regulated by 1,25-dihydroxyvitamin D3 in Parallel with Activated C-jun During Differentiation of Human Myeloid Leukemia Cells

Overview

Journal J Steroid Biochem Mol Biol

Specialties Biochemistry
Molecular Biology

Date 2010 Mar 16

PMID 20227498

Citations 4

Authors

Xuening Wang

George P Studzinski

Affiliations

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Abstract

The role of MAP kinase pathways in 1,25-dihydroxyvitamin D3 (1,25D)-induced differentiation of myeloid leukemia cells is well established, but the mechanisms by which 1,25D activates these pathways are not entirely clear. Following the finding that kinase suppressors of ras (KSR) 1 and 2 are directly regulated by 1,25D and participate in the monocytic differentiation process, we investigated if the COT1 oncogene (Tpl2 in the rat), known to interact with human KSR2 (hKSR2), is also involved in 1,25D-induced differentiation in leukemia cells. Here we report that the exposure of HL60 and U937 myeloid leukemia cells to 1,25D increases COT1 expression in a concentration-dependent manner. However, COT1 appears to have a differentiation-limiting role in these cells, as an exposure of HL60 and U937 cells to a pharmacological inhibitor of COT1 kinase activity, 4-(3-chloro-4-fluorophenylamino)-6-(pyridin-3-yl-methylamino-3-cyano-[1-7]-naphthyridine, results in increased 1,25D-induced differentiation. These findings provide an additional insight into the 1,25D-regulation of MAPK pathways that contribute to monocytic differentiation process of myeloid leukemia cells.

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References

Chiariello M, Marinissen M, Gutkind J . Multiple mitogen-activated protein kinase signaling pathways connect the cot oncoprotein to the c-jun promoter and to cellular transformation. Mol Cell Biol. 2000; 20(5):1747-58. PMC: 85357. DOI: 10.1128/MCB.20.5.1747-1758.2000. View

Hirata K, Taki H, Shinoda K, Hounoki H, Miyahara T, Tobe K . Inhibition of tumor progression locus 2 protein kinase suppresses receptor activator of nuclear factor-kappaB ligand-induced osteoclastogenesis through down-regulation of the c-Fos and nuclear factor of activated T cells c1 genes. Biol Pharm Bull. 2010; 33(1):133-7. DOI: 10.1248/bpb.33.133. View

Wang Q, Wang X, Studzinski G . Jun N-terminal kinase pathway enhances signaling of monocytic differentiation of human leukemia cells induced by 1,25-dihydroxyvitamin D3. J Cell Biochem. 2003; 89(6):1087-101. DOI: 10.1002/jcb.10595. View

Sundstrom C, Nilsson K . Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int J Cancer. 1976; 17(5):565-77. DOI: 10.1002/ijc.2910170504. View

Gallagher R, Collins S, Trujillo J, McCredie K, AHearn M, Tsai S . Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia. Blood. 1979; 54(3):713-33. View

Abe E, Miyaura C, Sakagami H, Takeda M, Konno K, Yamazaki T . Differentiation of mouse myeloid leukemia cells induced by 1 alpha,25-dihydroxyvitamin D3. Proc Natl Acad Sci U S A. 1981; 78(8):4990-4. PMC: 320317. DOI: 10.1073/pnas.78.8.4990. View

Mangelsdorf D, Koeffler H, Donaldson C, Pike J, Haussler M . 1,25-Dihydroxyvitamin D3-induced differentiation in a human promyelocytic leukemia cell line (HL-60): receptor-mediated maturation to macrophage-like cells. J Cell Biol. 1984; 98(2):391-8. PMC: 2113084. DOI: 10.1083/jcb.98.2.391. View

Studzinski G, Bhandal A, Brelvi Z . A system for monocytic differentiation of leukemic cells HL 60 by a short exposure to 1,25-dihydroxycholecalciferol. Proc Soc Exp Biol Med. 1985; 179(3):288-95. DOI: 10.3181/00379727-179-42098. View

Chomienne C, Ballerini P, Balitrand N, Daniel M, Fenaux P, Castaigne S . All-trans retinoic acid in acute promyelocytic leukemias. II. In vitro studies: structure-function relationship. Blood. 1990; 76(9):1710-7. View

10.

Miyoshi J, Higashi T, Mukai H, Ohuchi T, KAKUNAGA T . Structure and transforming potential of the human cot oncogene encoding a putative protein kinase. Mol Cell Biol. 1991; 11(8):4088-96. PMC: 361219. DOI: 10.1128/mcb.11.8.4088-4096.1991. View

11.

Patriotis C, Makris A, BEAR S, Tsichlis P . Tumor progression locus 2 (Tpl-2) encodes a protein kinase involved in the progression of rodent T-cell lymphomas and in T-cell activation. Proc Natl Acad Sci U S A. 1993; 90(6):2251-5. PMC: 46064. DOI: 10.1073/pnas.90.6.2251. View

12.

Patriotis C, Makris A, Chernoff J, Tsichlis P . Tpl-2 acts in concert with Ras and Raf-1 to activate mitogen-activated protein kinase. Proc Natl Acad Sci U S A. 1994; 91(21):9755-9. PMC: 44895. DOI: 10.1073/pnas.91.21.9755. View

13.

Wang X, Gardner J, Kheir A, Uskokovic M, Studzinski G . Synergistic induction of HL60 cell differentiation by ketoconazole and 1-desoxy analogues of vitamin D3. J Natl Cancer Inst. 1997; 89(16):1199-206. DOI: 10.1093/jnci/89.16.1199. View

14.

Hagemann D, Troppmair J, Rapp U . Cot protooncoprotein activates the dual specificity kinases MEK-1 and SEK-1 and induces differentiation of PC12 cells. Oncogene. 1999; 18(7):1391-400. DOI: 10.1038/sj.onc.1202431. View

15.

Das S, Cho J, Lambertz I, Kelliher M, Eliopoulos A, Du K . Tpl2/cot signals activate ERK, JNK, and NF-kappaB in a cell-type and stimulus-specific manner. J Biol Chem. 2005; 280(25):23748-57. DOI: 10.1074/jbc.M412837200. View

16.

Gavrin L, Green N, Hu Y, Janz K, Kaila N, Li H . Inhibition of Tpl2 kinase and TNF-alpha production with 1,7-naphthyridine-3-carbonitriles: synthesis and structure-activity relationships. Bioorg Med Chem Lett. 2005; 15(23):5288-92. DOI: 10.1016/j.bmcl.2005.08.029. View

17.

Wang T, Tavera-Mendoza L, Laperriere D, Libby E, MacLeod N, Nagai Y . Large-scale in silico and microarray-based identification of direct 1,25-dihydroxyvitamin D3 target genes. Mol Endocrinol. 2005; 19(11):2685-95. DOI: 10.1210/me.2005-0106. View

18.

Studzinski G, Wang X, Ji Y, Wang Q, Zhang Y, Kutner A . The rationale for deltanoids in therapy for myeloid leukemia: role of KSR-MAPK-C/EBP pathway. J Steroid Biochem Mol Biol. 2005; 97(1-2):47-55. PMC: 2814418. DOI: 10.1016/j.jsbmb.2005.06.010. View

19.

Hu Y, Green N, Gavrin L, Janz K, Kaila N, Li H . Inhibition of Tpl2 kinase and TNFalpha production with quinoline-3-carbonitriles for the treatment of rheumatoid arthritis. Bioorg Med Chem Lett. 2006; 16(23):6067-72. DOI: 10.1016/j.bmcl.2006.08.102. View

20.

Wang X, Wang T, White J, Studzinski G . Expression of human kinase suppressor of Ras 2 (hKSR-2) gene in HL60 leukemia cells is directly upregulated by 1,25-dihydroxyvitamin D(3) and is required for optimal cell differentiation. Exp Cell Res. 2007; 313(14):3034-45. PMC: 3351793. DOI: 10.1016/j.yexcr.2007.05.021. View