Visualizing Viral Dissemination in the Mouse Nervous System, Using a Green Fluorescent Protein-expressing Borna Disease Virus Vector

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2010 Mar 12

PMID 20219925

Citations 15

Authors

Andreas Ackermann

Timo Guelzow

Peter Staeheli

Urs Schneider

Bernd Heimrich

Affiliations

Soon will be listed here.

Abstract

Borna disease virus (BDV) frequently persists in the brain of infected animals. To analyze viral dissemination in the mouse nervous system, we generated a mouse-adapted virus that expresses green fluorescent protein (GFP). This viral vector supported GFP expression for up to 150 days and possessed an extraordinary staining capacity, visualizing complete dendritic arbors as well as individual axonal fibers of infected neurons. GFP-positive cells were first detected in cortical areas from where the virus disseminated through the entire central nervous system (CNS). Late in infection, GFP expression was found in the sciatic nerve, demonstrating viral spread from the central to the peripheral nervous system.

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References

Staeheli P, Sauder C, Hausmann J, Ehrensperger F, Schwemmle M . Epidemiology of Borna disease virus. J Gen Virol. 2000; 81(Pt 9):2123-2135. DOI: 10.1099/0022-1317-81-9-2123. View

Poenisch M, Wille S, Schneider U, Staeheli P . Second-site mutations in Borna disease virus overexpressing viral accessory protein X. J Gen Virol. 2009; 90(Pt 8):1932-1936. DOI: 10.1099/vir.0.011841-0. View

de la Torre J . Bornavirus and the brain. J Infect Dis. 2002; 186 Suppl 2:S241-7. DOI: 10.1086/344936. View

Sauder C, Staeheli P . Rat model of borna disease virus transmission: epidemiological implications. J Virol. 2003; 77(23):12886-90. PMC: 262589. DOI: 10.1128/jvi.77.23.12886-12890.2003. View

Kao M, Ludwig H, Gosztonyi G . Adaptation of Borna disease virus to the mouse. J Gen Virol. 1984; 65 ( Pt 10):1845-9. DOI: 10.1099/0022-1317-65-10-1845. View

Herzog S, Kompter C, Frese K, Rott R . Replication of Borna disease virus in rats: age-dependent differences in tissue distribution. Med Microbiol Immunol. 1984; 173(4):171-7. DOI: 10.1007/BF02122108. View

Morales J, Herzog S, Kompter C, Frese K, Rott R . Axonal transport of Borna disease virus along olfactory pathways in spontaneously and experimentally infected rats. Med Microbiol Immunol. 1988; 177(2):51-68. DOI: 10.1007/BF00189527. View

Celio M . Calbindin D-28k and parvalbumin in the rat nervous system. Neuroscience. 1990; 35(2):375-475. DOI: 10.1016/0306-4522(90)90091-h. View

Briese T, Schneemann A, Lewis A, Park Y, Kim S, Ludwig H . Genomic organization of Borna disease virus. Proc Natl Acad Sci U S A. 1994; 91(10):4362-6. PMC: 43785. DOI: 10.1073/pnas.91.10.4362. View

10.

Stitz L, Noske K, Planz O, Furrer E, Lipkin W, Bilzer T . A functional role for neutralizing antibodies in Borna disease: influence on virus tropism outside the central nervous system. J Virol. 1998; 72(11):8884-92. PMC: 110305. DOI: 10.1128/JVI.72.11.8884-8892.1998. View

11.

Schneider U, Schwemmle M, Staeheli P . Genome trimming: a unique strategy for replication control employed by Borna disease virus. Proc Natl Acad Sci U S A. 2005; 102(9):3441-6. PMC: 552903. DOI: 10.1073/pnas.0405965102. View

12.

Martin A, Staeheli P, Schneider U . RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication. J Virol. 2006; 80(12):5708-15. PMC: 1472609. DOI: 10.1128/JVI.02389-05. View

13.

Schneider U, Ackermann A, Staeheli P . A Borna disease virus vector for expression of foreign genes in neurons of rodents. J Virol. 2007; 81(13):7293-6. PMC: 1933307. DOI: 10.1128/JVI.02467-06. View

14.

Ackermann A, Staeheli P, Schneider U . Adaptation of Borna disease virus to new host species attributed to altered regulation of viral polymerase activity. J Virol. 2007; 81(15):7933-40. PMC: 1951315. DOI: 10.1128/JVI.00334-07. View

15.

Nishino Y, Kobasa D, Rubin S, Pletnikov M, Carbone K . Enhanced neurovirulence of borna disease virus variants associated with nucleotide changes in the glycoprotein and L polymerase genes. J Virol. 2002; 76(17):8650-8. PMC: 136970. DOI: 10.1128/jvi.76.17.8650-8658.2002. View