Inhibition of Gene Expression by RNase P

Overview

Journal N Biotechnol

Publisher Elsevier

Specialties Biomedical Engineering
Biotechnology
Molecular Biology

Date 2010 Mar 10

PMID 20211282

Citations 22

Authors

Eirik Wasmuth Lundblad

Sidney Altman

Affiliations

Soon will be listed here.

Abstract

The ability to interfere with gene expression is of crucial importance to unravel the function of genes and is also a promising therapeutic strategy. Here we discuss methodologies for inhibition of target RNAs based on the cleavage activity of the essential enzyme, Ribonuclease P (RNase P). RNase P-mediated cleavage of target RNAs can be directed by external guide sequences (EGSs) or by the use of the catalytic M1 RNA from E. coli linked to a guide sequence (M1GSs). These are not only basic tools for functional genetic studies in prokaryotic and eukaryotic cells but also promising antibacterial, anticancer and antiviral agents.

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