Toxoplasma Secreting Cre Recombinase for Analysis of Host-parasite Interactions

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Mar 9

PMID 20208532

Citations 51

Authors

Anita A Koshy

Ashley E Fouts

Melissa B Lodoen

Ozan Alkan

Helen M Blau

John C Boothroyd

Affiliations

Soon will be listed here.

Abstract

We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.

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