Complete Genome Sequences of Avian Paramyxovirus Serotype 6 Prototype Strain Hong Kong and a Recent Novel Strain from Italy: Evidence for the Existence of Subgroups Within the Serotype

Overview

Journal Virus Res

Specialty Microbiology

Date 2010 Mar 9

PMID 20206652

Citations 26

Authors

Sa Xiao

Madhuri Subbiah

Sachin Kumar

Roberta De Nardi

Calogero Terregino

Peter L Collins

Siba K Samal

Affiliations

Soon will be listed here.

Abstract

Complete genome sequences were determined for two strains of avian paramyxovirus serotype 6 (APMV-6): the prototype Hong Kong (HK) strain and a more recent isolate from Italy (IT4524-2). The genome length of strain HK is 16236 nucleotide (nt), which is the same as for the other two APMV-6 strains (FE and TW) that have been reported to date, whereas that of strain IT4524-2 is 16230 nt. The length difference in strain IT4524-2 is due to a 6-nt deletion in the downstream untranslated region of the F gene. All of these viruses follow the "rule of six". Each genome consists of seven genes in the order of 3'N-P-M-F-SH-HN-L5', which differs from other APMV serotypes in containing an additional gene encoding the small hydrophobic (SH) protein. Sequence comparisons revealed that strain IT4524-2 shares an unexpectedly low level of genome nt sequence identity (70%) and aggregate predicted amino acid (aa) sequence identity (79%) with other three strains, which in contrast are more closely related to each other with nt sequence 94-98% nt identity and 90-100% aggregate aa identity. Sequence analysis of the F-SH-HN genome region of two other recent Italian isolates showed that they fall in the HK/FE/TW group. The predicted signal peptide of IT4524-2 F protein lacks the N-terminal first 10 aa that are present in the other five strains. Also, the F protein cleavage site of strain IT4524-2, REPR downward arrow L, has two dibasic aa (arginine, R) compared to the monobasic F protein cleavage site of PEPR downward arrow L in the other strains. Reciprocal cross-hemagglutination inhibition (HI) assays using post-infection chicken sera indicated that strain IT4524-2 is antigenically related to the other APMV-6 strains, but with 4- to 8-fold lower HI tiers for the test sera between strain IT4524-2 and the other APMV-6 strains. Taken together, our results indicated that the APMV-6 strains represents a single serotype with two subgroups that differ substantially based on nt and aa sequences and can be distinguished by HI assay.

Citing Articles

Discovery of Avian Paramyxoviruses APMV-1 and APMV-6 in Shorebirds and Waterfowl in Southern Ukraine.

Klink A, Rula O, Sushko M, Bezymennyi M, Mezinov O, Gaidash O Viruses. 2023; 15(3).

PMID: 36992408 PMC: 10058161. DOI: 10.3390/v15030699.

Avian Paramyxovirus 4 Antitumor Activity Leads to Complete Remissions and Long-term Protective Memory in Preclinical Melanoma and Colon Carcinoma Models.

Javaheri A, Bykov Y, Mena I, Garcia-Sastre A, Cuadrado-Castano S Cancer Res Commun. 2022; 2(7):602-615.

PMID: 35937459 PMC: 9351398. DOI: 10.1158/2767-9764.crc-22-0025.

Putative Novel Avian Paramyxovirus (AMPV) and Reidentification of APMV-2 and APMV-6 to the Species Level Based on Wild Bird Surveillance (United States, 2016-2018).

Young K, Stephens J, Poulson R, Stallknecht D, Dimitrov K, Butt S Appl Environ Microbiol. 2022; 88(11):e0046622.

PMID: 35612300 PMC: 9195946. DOI: 10.1128/aem.00466-22.

Genetic and Antigenic Characterization of Avian Avulavirus Type 6 (AAvV-6) Circulating in Canadian Wild Birds (2005-2017).

Hisanaga T, Soos C, Lewis N, Lung O, Suderman M, Berhane Y Viruses. 2021; 13(4).

PMID: 33805157 PMC: 8064105. DOI: 10.3390/v13040543.

Molecular evolution and genetic variations of V and W proteins derived by RNA editing in Avian Paramyxoviruses.

Rao P, Gandham R, Subbiah M Sci Rep. 2020; 10(1):9532.

PMID: 32533018 PMC: 7293227. DOI: 10.1038/s41598-020-66252-x.

References

Schrempf S, Froeschke M, Giroglou T, von Laer D, Dobberstein B . Signal peptide requirements for lymphocytic choriomeningitis virus glycoprotein C maturation and virus infectivity. J Virol. 2007; 81(22):12515-24. PMC: 2168972. DOI: 10.1128/JVI.01481-07. View

Evans S, Belsham G, Barrett T . The role of the 5' nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus. Virology. 1990; 177(1):317-23. DOI: 10.1016/0042-6822(90)90486-b. View

Brudno M, Do C, Cooper G, Kim M, Davydov E, Green E . LAGAN and Multi-LAGAN: efficient tools for large-scale multiple alignment of genomic DNA. Genome Res. 2003; 13(4):721-31. PMC: 430158. DOI: 10.1101/gr.926603. View

Johnson Jr P, Olmsted R, Prince G, Murphy B, Alling D, Walsh E . Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity. J Virol. 1987; 61(10):3163-6. PMC: 255893. DOI: 10.1128/JVI.61.10.3163-3166.1987. View

Lipkind M, Shihmanter E . Antigenic relationships between avian paramyxoviruses. I. Quantitative characteristics based on hemagglutination and neuraminidase inhibition tests. Arch Virol. 1986; 89(1-4):89-111. DOI: 10.1007/BF01309882. View

Xiao S, Paldurai A, Nayak B, Subbiah M, Collins P, Samal S . Complete genome sequence of avian paramyxovirus type 7 (strain Tennessee) and comparison with other paramyxoviruses. Virus Res. 2009; 145(1):80-91. PMC: 3292215. DOI: 10.1016/j.virusres.2009.06.003. View

Lindemann D, Pietschmann T, Picard-Maureau M, Berg A, Heinkelein M, Thurow J . A particle-associated glycoprotein signal peptide essential for virus maturation and infectivity. J Virol. 2001; 75(13):5762-71. PMC: 114292. DOI: 10.1128/JVI.75.13.5762-5771.2001. View

Samuel A, Kumar S, Madhuri S, Collins P, Samal S . Complete sequence of the genome of avian paramyxovirus type 9 and comparison with other paramyxoviruses. Virus Res. 2009; 142(1-2):10-8. PMC: 2782688. DOI: 10.1016/j.virusres.2008.12.016. View

Nayak B, Kumar S, Collins P, Samal S . Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75. Virol J. 2008; 5:124. PMC: 2577636. DOI: 10.1186/1743-422X-5-124. View

10.

Jung A, Grund C, Muller I, Rautenschlein S . Avian paramyxovirus serotype 3 infection in Neopsephotus, Cyanoramphus, and Neophema species. J Avian Med Surg. 2009; 23(3):205-8. DOI: 10.1647/2008-022.1. View

11.

Saif Y, Mohan R, Ward L, Senne D, Panigrahy B, Dearth R . Natural and experimental infection of turkeys with avian paramyxovirus-7. Avian Dis. 1997; 41(2):326-9. View

12.

Gan S, Ng L, Lin X, Gong X, Torres J . Structure and ion channel activity of the human respiratory syncytial virus (hRSV) small hydrophobic protein transmembrane domain. Protein Sci. 2008; 17(5):813-20. PMC: 2327286. DOI: 10.1110/ps.073366208. View

13.

Czegledi A, Ujvari D, Somogyi E, Wehmann E, Werner O, Lomniczi B . Third genome size category of avian paramyxovirus serotype 1 (Newcastle disease virus) and evolutionary implications. Virus Res. 2006; 120(1-2):36-48. DOI: 10.1016/j.virusres.2005.11.009. View

14.

Stanislawek W, Wilks C, Meers J, Horner G, Alexander D, Manvell R . Avian paramyxoviruses and influenza viruses isolated from mallard ducks (Anas platyrhynchos) in New Zealand. Arch Virol. 2002; 147(7):1287-302. DOI: 10.1007/s00705-002-0818-2. View

15.

Yu M, Hansson E, Shiell B, Michalski W, Eaton B, Wang L . Sequence analysis of the Hendra virus nucleoprotein gene: comparison with other members of the subfamily Paramyxovirinae. J Gen Virol. 1998; 79 ( Pt 7):1775-80. DOI: 10.1099/0022-1317-79-7-1775. View

16.

Bendtsen J, Nielsen H, von Heijne G, Brunak S . Improved prediction of signal peptides: SignalP 3.0. J Mol Biol. 2004; 340(4):783-95. DOI: 10.1016/j.jmb.2004.05.028. View

17.

Warke A, Stallknecht D, Williams S, Pritchard N, Mundt E . Comparative study on the pathogenicity and immunogenicity of wild bird isolates of avian paramyxovirus 2, 4, and 6 in chickens. Avian Pathol. 2008; 37(4):429-34. DOI: 10.1080/03079450802216645. View

18.

Morrison T, McQuain C, Sergel T, McGinnes L, Reitter J . The role of the amino terminus of F1 of the Newcastle disease virus fusion protein in cleavage and fusion. Virology. 1993; 193(2):997-1000. DOI: 10.1006/viro.1993.1214. View

19.

Zhang G, Zhao J, Wang M . Serological survey on prevalence of antibodies to avian paramyxovirus serotype 2 in China. Avian Dis. 2007; 51(1):137-9. DOI: 10.1637/0005-2086(2007)051[0137:SSOPOA]2.0.CO;2. View

20.

Biacchesi S, Skiadopoulos M, Boivin G, Hanson C, Murphy B, Collins P . Genetic diversity between human metapneumovirus subgroups. Virology. 2003; 315(1):1-9. DOI: 10.1016/s0042-6822(03)00528-2. View