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Reversible Ponceau Staining As a Loading Control Alternative to Actin in Western Blots

Overview
Journal Anal Biochem
Publisher Elsevier
Specialty Biochemistry
Date 2010 Mar 9
PMID 20206115
Citations 315
Authors
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Abstract

It is becoming standard practice to measure a housekeeping gene, typically actin, in Western blots, as it is the rule in RNA blots. We have applied reversible Ponceau staining to check equal loading of gels and measured actin in parallel under different conditions. Our results show that densitometric analysis is comparable with both techniques. Therefore, routine quantitation of Ponceau staining before antibody probing is validated as an alternative to actin blotting.

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