Quality Evaluation of Mycelial Antrodia Camphorata Using High-performance Liquid Chromatography (HPLC) Coupled with Diode Array Detector and Mass Spectrometry (DAD-MS)

Overview

Journal Chin Med

Publisher Biomed Central

Specialty Biomedical Engineering

Date 2010 Mar 9

PMID 20205903

Citations 4

Authors

Sandy Shuo Zhao

Kelvin Sze-Yin Leung

Affiliations

Soon will be listed here.

Abstract

Background: Antrodia camphorata (AC) is an important fungus native to Taiwanese forested regions. Scientific studies have demonstrated that extracts of AC possess a variety of pharmacological functions. This study aims to identify the full profile fingerprint of nucleosides and nucleobases in mycelial AC and to assess the quality of two commercial mycelial AC products.

Methods: High-performance liquid chromatography coupled with diode array detector and mass spectrometry was employed to identify the major components in mycelial AC. The chemical separation was carried out using a gradient program on a reverse phase Alltima C18 AQ analytical column (250 x 4.6 mm, 5 microm) with the mobile phase consisting of deionized water and methanol.

Results: Ten nucleosides and nucleobases, two maleimide derivatives, and a sterol were identified as the major constituents in mycelial AC. These groups of chemical compounds constitute the first chromatographic fingerprint as an index for quality assessment of this medicinal fungus.

Conclusions: This study provides the first chromatographic fingerprint to assess the quality of mycelial AC.

Citing Articles

Synthesis of Natural (-)-Antrocin and its Enantiomer via Stereoselective Aldol Reaction.

Angamuthu V, Tai D Molecules. 2020; 25(4).

PMID: 32075004 PMC: 7070359. DOI: 10.3390/molecules25040831.

Extraction of bioactive ingredients from fruiting bodies of assisted by high hydrostatic pressure.

Huang H, Chen B, Wang C J Food Sci Technol. 2019; 56(9):3988-3997.

PMID: 31477970 PMC: 6706480. DOI: 10.1007/s13197-019-03867-7.

Enhancing the Anticancer Activity of in Hepatocellular Carcinoma Cells via Cocultivation With Ginger: The Impact on Cancer Cell Survival Pathways.

Chen S, Lee Y, Hsieh M, Omar H, Teng Y, Lin C Front Pharmacol. 2018; 9:780.

PMID: 30072899 PMC: 6058215. DOI: 10.3389/fphar.2018.00780.

Chemical quality evaluation of Antrodia cinnamomea fruiting bodies using phytomics similarity index analysis.

Chung C, Yeh S, Tseng H, Siu M, Lee K J Food Drug Anal. 2017; 24(1):173-178.

PMID: 28911401 PMC: 9345421. DOI: 10.1016/j.jfda.2015.01.008.

References

Huang R, Chen C, Chen H, Hsu W, Lu M . Antrodia camphorata polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol Lett. 2002; 209(1):63-7. DOI: 10.1111/j.1574-6968.2002.tb11110.x. View

Ao Z, Xu Z, Lu Z, Xu H, Zhang X, Dou W . Niuchangchih (Antrodia camphorata) and its potential in treating liver diseases. J Ethnopharmacol. 2008; 121(2):194-212. DOI: 10.1016/j.jep.2008.10.039. View

Nakamura N, Hirakawa A, Gao J, Kakuda H, Shiro M, Komatsu Y . Five new maleic and succinic acid derivatives from the mycelium of Antrodia camphorata and their cytotoxic effects on LLC tumor cell line. J Nat Prod. 2004; 67(1):46-8. DOI: 10.1021/np030293k. View

Cheng J, Yang C, Cheng C, Wang Y, Huang N, Lu M . Characterization and functional study of Antrodia camphorata lipopolysaccharide. J Agric Food Chem. 2005; 53(2):469-74. DOI: 10.1021/jf049281a. View

Huang N, Cheng J, Lai W, Lu M . Antrodia camphorata prevents rat pheochromocytoma cells from serum deprivation-induced apoptosis. FEMS Microbiol Lett. 2005; 244(1):213-9. DOI: 10.1016/j.femsle.2005.01.048. View

Cheng J, Huang N, Chang T, Wang D, Lu M . Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sci. 2005; 76(26):3029-42. DOI: 10.1016/j.lfs.2004.11.023. View

Chen S, Lu M, Cheng J, Wang D . Antiangiogenic activities of polysaccharides isolated from medicinal fungi. FEMS Microbiol Lett. 2005; 249(2):247-54. DOI: 10.1016/j.femsle.2005.06.033. View

Hsu F, Chou C, Chang Y, Chang T, Lu M . Promotion of hyphal growth and underlying chemical changes in Antrodia camphorata by host factors from Cinnamomum camphora. Int J Food Microbiol. 2005; 106(1):32-8. DOI: 10.1016/j.ijfoodmicro.2005.07.003. View

Lu M, Cheng J, Lai W, Lin Y, Huang N . Adenosine as an active component of Antrodia cinnamomea that prevents rat PC12 cells from serum deprivation-induced apoptosis through the activation of adenosine A(2A) receptors. Life Sci. 2006; 79(3):252-8. DOI: 10.1016/j.lfs.2005.12.042. View

10.

Chen C, Shiao Y, Lin R, Shao Y, Lai M, Lin C . Neuroprotective diterpenes from the fruiting body of Antrodia camphorata. J Nat Prod. 2006; 69(4):689-91. DOI: 10.1021/np0581263. View

11.

Gao J, Leung K, Wang Y, Lai C, Li S, Hu L . Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC-DAD-MS. J Pharm Biomed Anal. 2007; 44(3):807-11. DOI: 10.1016/j.jpba.2007.03.012. View

12.

Chen C, Liu Y, Ker Y, Wu Y, Lai E, Chyau C . Chemical characterization and anti-inflammatory effect of polysaccharides fractionated from submerge-cultured Antrodia camphorata mycelia. J Agric Food Chem. 2007; 55(13):5007-12. DOI: 10.1021/jf063484c. View

13.

Chen J, Lin W, Liao C, Shieh P . Anti-inflammatory benzenoids from Antrodia camphorata. J Nat Prod. 2007; 70(6):989-92. DOI: 10.1021/np070045e. View

14.

Lu M, Cheng J, Lai W, Lin Y, Huang N . Fermented Antrodia cinnamomea extract protects rat PC12 cells from serum deprivation-induced apoptosis: the role of the MAPK family. J Agric Food Chem. 2008; 56(3):865-74. DOI: 10.1021/jf072828b. View

15.

Wu M, Cheng M, Wang B, Yech Y, Lai J, Kuo Y . Maleimide and maleic anhydride derivatives from the mycelia of Antrodia cinnamomea and their nitric oxide inhibitory activities in macrophages. J Nat Prod. 2008; 71(7):1258-61. DOI: 10.1021/np070634k. View

16.

Xie P, Leung A . Understanding the traditional aspect of Chinese medicine in order to achieve meaningful quality control of Chinese materia medica. J Chromatogr A. 2008; 1216(11):1933-40. DOI: 10.1016/j.chroma.2008.08.045. View

17.

Wang G, Tseng H, Chou C, Tsai T, Chen C, Lu M . The vasorelaxation of Antrodia camphorata mycelia: involvement of endothelial Ca(2+)-NO-cGMP pathway. Life Sci. 2003; 73(21):2769-83. DOI: 10.1016/s0024-3205(03)00669-6. View