Isolation and Nucleotide Sequence of the Aspergillus Restrictus Gene Coding for the Ribonucleolytic Toxin Restrictocin and Its Expression in Aspergillus Nidulans: the Leader Sequence Protects Producing Strains from Suicide

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1991 Mar 11

PMID 2020539

Citations 13

Authors

B Lamy

J Davies

Affiliations

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Abstract

We describe the cloning and characterization of the gene coding for the ribotoxin restrictocin, from Aspergillus restrictus (gene res, EMBL accession Number X56176). This toxin is a potent inhibitor of protein synthesis in eucaryotes and is of potential interest as a component of immunotoxins. To analyze the mechanism of self-protection in the producing organism, the res gene was cloned into the vector pFB39 and introduced into Aspergillus nidulans. The secretion of active restrictocin from transformants suggests that the pro-toxin is not an active nuclease but is activated during the process of secretion.

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