Functional Centromeres in Soybean Include Two Distinct Tandem Repeats and a Retrotransposon

Overview

Journal Chromosome Res

Date 2010 Mar 6

PMID 20204495

Citations 40

Authors

Ahmet L Tek

Kazunari Kashihara

Minoru Murata

Kiyotaka Nagaki

Affiliations

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Abstract

The centromere as a kinetochore assembly site is fundamental to the partitioning of genetic material during cell division. In order to determine the functional centromeres of soybean, we characterized the soybean centromere-specific histone H3 (GmCENH3) protein and developed an antibody against the N-terminal end. Using this antibody, we cloned centromere-associated DNA sequences by chromatin immunoprecipitation. Our analyses indicate that soybean centromeres are composed of two distinct satellite repeats (GmCent-1 and GmCent-4) and retrotransposon-related sequences (GmCR). The possible allopolyploid origin of the soybean genome is discussed in view of the centromeric satellite sequences present.

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