A Flow Cytometry-based Assay for Measuring Invasion of Red Blood Cells by Plasmodium Falciparum

Overview

Journal Am J Hematol

Specialty Hematology

Date 2010 Mar 3

PMID 20196166

Citations 44

Authors

Amy K Bei

Tiffany M DeSimone

Aida S Badiane

Ambroise D Ahouidi

Tandakha Dieye

Daouda Ndiaye

Ousmane Sarr

Omar Ndir

Souleymane Mboup

Manoj T Duraisingh

Affiliations

Soon will be listed here.

Abstract

Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion.

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