Auxin Biosynthesis and Its Role in Plant Development

Overview

Journal Annu Rev Plant Biol

Specialties Biology
Molecular Biology

Date 2010 Mar 3

PMID 20192736

Citations 471

Authors

Yunde Zhao

Affiliations

Soon will be listed here.

Abstract

Indole-3-acetic acid (IAA), the main auxin in higher plants, has profound effects on plant growth and development. Both plants and some plant pathogens can produce IAA to modulate plant growth. Although the genes and biochemical reactions for auxin biosynthesis in some plant pathogens are well understood, elucidation of the mechanisms by which plants produce auxin has proven to be difficult. So far, no single complete pathway of de novo auxin biosynthesis in plants has been firmly established. However, recent studies have led to the discoveries of several genes in tryptophan-dependent auxin biosynthesis pathways. Recent findings have also determined that local auxin biosynthesis plays essential roles in many developmental processes including gametogenesis, embryogenesis, seedling growth, vascular patterning, and flower development. In this review, I summarize the recent advances in dissecting auxin biosynthetic pathways and how the understanding of auxin biosynthesis provides a crucial angle for analyzing the mechanisms of plant development.

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References

Przemeck G, Mattsson J, Hardtke C, Sung Z, Berleth T . Studies on the role of the Arabidopsis gene MONOPTEROS in vascular development and plant cell axialization. Planta. 1996; 200(2):229-37. DOI: 10.1007/BF00208313. View

Stone S, Braybrook S, Paula S, Kwong L, Meuser J, Pelletier J . Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis. Proc Natl Acad Sci U S A. 2008; 105(8):3151-6. PMC: 2268600. DOI: 10.1073/pnas.0712364105. View

Cheng Y, Dai X, Zhao Y . Auxin biosynthesis by the YUCCA flavin monooxygenases controls the formation of floral organs and vascular tissues in Arabidopsis. Genes Dev. 2006; 20(13):1790-9. PMC: 1522075. DOI: 10.1101/gad.1415106. View

Treml B, Winderl S, Radykewicz R, Herz M, Schweizer G, Hutzler P . The gene ENHANCER OF PINOID controls cotyledon development in the Arabidopsis embryo. Development. 2005; 132(18):4063-74. DOI: 10.1242/dev.01969. View

Li L, Hou X, Tsuge T, Ding M, Aoyama T, Oka A . The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis. Plant Cell Rep. 2007; 27(3):575-84. DOI: 10.1007/s00299-007-0458-9. View

Vanneste S, Friml J . Auxin: a trigger for change in plant development. Cell. 2009; 136(6):1005-16. DOI: 10.1016/j.cell.2009.03.001. View

Furutani M, Kajiwara T, Kato T, Treml B, Stockum C, Torres-Ruiz R . The gene MACCHI-BOU 4/ENHANCER OF PINOID encodes a NPH3-like protein and reveals similarities between organogenesis and phototropism at the molecular level. Development. 2007; 134(21):3849-59. DOI: 10.1242/dev.009654. View

Hardtke C, Berleth T . The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development. EMBO J. 1998; 17(5):1405-11. PMC: 1170488. DOI: 10.1093/emboj/17.5.1405. View

Sugawara S, Hishiyama S, Jikumaru Y, Hanada A, Nishimura T, Koshiba T . Biochemical analyses of indole-3-acetaldoxime-dependent auxin biosynthesis in Arabidopsis. Proc Natl Acad Sci U S A. 2009; 106(13):5430-5. PMC: 2664063. DOI: 10.1073/pnas.0811226106. View

10.

Woodward C, Bemis S, Hill E, Sawa S, Koshiba T, Torii K . Interaction of auxin and ERECTA in elaborating Arabidopsis inflorescence architecture revealed by the activation tagging of a new member of the YUCCA family putative flavin monooxygenases. Plant Physiol. 2005; 139(1):192-203. PMC: 1203369. DOI: 10.1104/pp.105.063495. View

11.

Cheng Y, Dai X, Zhao Y . Auxin synthesized by the YUCCA flavin monooxygenases is essential for embryogenesis and leaf formation in Arabidopsis. Plant Cell. 2007; 19(8):2430-9. PMC: 2002601. DOI: 10.1105/tpc.107.053009. View

12.

Dai X, Hayashi K, Nozaki H, Cheng Y, Zhao Y . Genetic and chemical analyses of the action mechanisms of sirtinol in Arabidopsis. Proc Natl Acad Sci U S A. 2005; 102(8):3129-34. PMC: 549487. DOI: 10.1073/pnas.0500185102. View

13.

Li L, Qin G, Tsuge T, Hou X, Ding M, Aoyama T . SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis. New Phytol. 2008; 179(3):751-764. DOI: 10.1111/j.1469-8137.2008.02514.x. View

14.

Christensen S, Dagenais N, Chory J, Weigel D . Regulation of auxin response by the protein kinase PINOID. Cell. 2000; 100(4):469-78. DOI: 10.1016/s0092-8674(00)80682-0. View

15.

Vieten A, Vanneste S, Wisniewska J, Benkova E, Benjamins R, Beeckman T . Functional redundancy of PIN proteins is accompanied by auxin-dependent cross-regulation of PIN expression. Development. 2005; 132(20):4521-31. DOI: 10.1242/dev.02027. View

16.

Ikeda Y, Men S, Fischer U, Stepanova A, Alonso J, Ljung K . Local auxin biosynthesis modulates gradient-directed planar polarity in Arabidopsis. Nat Cell Biol. 2009; 11(6):731-8. DOI: 10.1038/ncb1879. View

17.

Paciorek T, Zazimalova E, Ruthardt N, Petrasek J, Stierhof Y, Kleine-Vehn J . Auxin inhibits endocytosis and promotes its own efflux from cells. Nature. 2005; 435(7046):1251-6. DOI: 10.1038/nature03633. View

18.

Sun J, Xu Y, Ye S, Jiang H, Chen Q, Liu F . Arabidopsis ASA1 is important for jasmonate-mediated regulation of auxin biosynthesis and transport during lateral root formation. Plant Cell. 2009; 21(5):1495-511. PMC: 2700526. DOI: 10.1105/tpc.108.064303. View

19.

Normanly J, GRISAFI P, Fink G, Bartel B . Arabidopsis mutants resistant to the auxin effects of indole-3-acetonitrile are defective in the nitrilase encoded by the NIT1 gene. Plant Cell. 1997; 9(10):1781-90. PMC: 157021. DOI: 10.1105/tpc.9.10.1781. View

20.

Bartel B . AUXIN BIOSYNTHESIS. Annu Rev Plant Physiol Plant Mol Biol. 1997; 48:51-66. DOI: 10.1146/annurev.arplant.48.1.51. View