Production of Recombinant Proteins from Protozoan Parasites

Overview

Journal Trends Parasitol

Specialty Parasitology

Date 2010 Mar 2

PMID 20189877

Citations 18

Authors

Jose A Fernandez-Robledo

Gerardo R Vasta

Affiliations

Soon will be listed here.

Abstract

Although the past decade has witnessed sequencing from an increasing number of parasites, modern high-throughput DNA sequencing technologies have the potential to generate complete genome sequences at even higher rates. Along with the discovery of genes that might constitute potential targets for chemotherapy or vaccination, the need for novel protein expression platforms has become a pressing matter. In addition to reviewing the advantages and limitations of the currently available and emerging expression systems, we discuss novel approaches that could overcome current limitations, including the 'pseudoparasite' concept, an expression platform in which the choice of the surrogate organism is based on its phylogenetic affinity to the target parasite, while taking advantage of the whole engineered organism as a vaccination adjuvant.

Citing Articles

Insights into Peptidyl-Prolyl - Isomerases from Clinically Important Protozoans: From Structure to Potential Biotechnological Applications.

Aranda-Chan V, Cardenas-Guerra R, Otero-Pedraza A, Pacindo-Cabrales E, Flores-Pucheta C, Montes-Flores O Pathogens. 2024; 13(8).

PMID: 39204244 PMC: 11357558. DOI: 10.3390/pathogens13080644.

Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis.

Solowinska K, Holec-Gasior L Microorganisms. 2024; 12(8).

PMID: 39203573 PMC: 11357668. DOI: 10.3390/microorganisms12081731.

Recombinant AMA1 Virus-like Particle Antigen for Serodiagnosis of Infection.

Kim M, Chu K, Mao J, Kang H, Eom G, Yoon K Biomedicines. 2022; 10(11).

PMID: 36359332 PMC: 9687185. DOI: 10.3390/biomedicines10112812.

Construction of Fluorescent Immunosensor Quenchbody to Detect His-Tagged Recombinant Proteins Produced in Bioprocess.

Ning X, Yasuda T, Kitaguchi T, Ueda H Sensors (Basel). 2021; 21(15).

PMID: 34372230 PMC: 8347774. DOI: 10.3390/s21154993.

Functional Comparison of Blood-Stage Malaria Vaccine Candidate Antigens.

Illingworth J, Alanine D, Brown R, Marshall J, Bartlett H, Silk S Front Immunol. 2019; 10:1254.

PMID: 31214195 PMC: 6558156. DOI: 10.3389/fimmu.2019.01254.

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