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Production of Recombinant Proteins from Protozoan Parasites

Overview
Specialty Parasitology
Date 2010 Mar 2
PMID 20189877
Citations 18
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Abstract

Although the past decade has witnessed sequencing from an increasing number of parasites, modern high-throughput DNA sequencing technologies have the potential to generate complete genome sequences at even higher rates. Along with the discovery of genes that might constitute potential targets for chemotherapy or vaccination, the need for novel protein expression platforms has become a pressing matter. In addition to reviewing the advantages and limitations of the currently available and emerging expression systems, we discuss novel approaches that could overcome current limitations, including the 'pseudoparasite' concept, an expression platform in which the choice of the surrogate organism is based on its phylogenetic affinity to the target parasite, while taking advantage of the whole engineered organism as a vaccination adjuvant.

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References
1.
Tsuboi T, Takeo S, Iriko H, Jin L, Tsuchimochi M, Matsuda S . Wheat germ cell-free system-based production of malaria proteins for discovery of novel vaccine candidates. Infect Immun. 2008; 76(4):1702-8. PMC: 2292889. DOI: 10.1128/IAI.01539-07. View

2.
Awale M, Kumar V, Saravanan P, Mohan C . Homology modeling and atomic level binding study of Leishmania MAPK with inhibitors. J Mol Model. 2009; 16(3):475-88. DOI: 10.1007/s00894-009-0565-3. View

3.
Seitzer U, Beyer D, Kullmann B, Bakheit M, Ahmed J . Evaluation of Theileria annulata recombinant immunodominant proteins for the development of ELISA. Transbound Emerg Dis. 2008; 55(5-6):244-8. DOI: 10.1111/j.1865-1682.2008.01030.x. View

4.
Walther M, Thompson F, Dunachie S, Keating S, Todryk S, Berthoud T . Safety, immunogenicity, and efficacy of prime-boost immunization with recombinant poxvirus FP9 and modified vaccinia virus Ankara encoding the full-length Plasmodium falciparum circumsporozoite protein. Infect Immun. 2006; 74(5):2706-16. PMC: 1459746. DOI: 10.1128/IAI.74.5.2706-2716.2006. View

5.
Gaertig J, Gao Y, Tishgarten T, Clark T, Dickerson H . Surface display of a parasite antigen in the ciliate Tetrahymena thermophila. Nat Biotechnol. 1999; 17(5):462-5. DOI: 10.1038/8638. View