Discovery of Cyclooxygenase Inhibitors from Medicinal Plants Used to Treat Inflammation

Overview

Journal Pharmacol Res

Publisher Elsevier

Specialty Pharmacology

Date 2010 Mar 2

PMID 20188172

Citations 29

Authors

Hongmei Cao

Rui Yu

Yongsoo Choi

Zhong-Ze Ma

Hongjie Zhang

Wei Xiang

David Yue-Wei Lee

Brian M Berman

Kamal D Moudgil

Harry H S Fong

Richard B van Breemen

Affiliations

Soon will be listed here.

Abstract

Eleven authenticated botanicals used in the traditional Chinese medicine Huo-Luo-Xiao-Ling Dan were screened for ligands to cyclooxygenase (COX) using pulsed ultrafiltration liquid chromatography-mass spectrometry, and a mass spectrometry-based enzyme assay was used to determine the concentration of each of 17 ligands that inhibited COX-1 or COX-2 by 50% (IC(50)). Acetyl-11-keto-beta-boswellic acid, beta-boswellic acid, acetyl-alpha-boswellic acid, acetyl-beta-boswellic acid, and betulinic acid were COX-1 selective inhibitors with IC(50) values of approximately 10 microM. Senkyunolide O and cryptotanshinone were COX-2 selective inhibitors with IC(50) values of 5 microM and 22 microM, respectively. Roburic acid and phenethyl-trans-ferulate inhibited COX-1 and COX-2 equally. COX inhibition and the IC(50) values of most of these natural product ligands have not been reported previously.

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