A Nodule-specific Protein Secretory Pathway Required for Nitrogen-fixing Symbiosis

Overview

Journal Science

Specialty Science

Date 2010 Feb 27

PMID 20185723

Citations 107

Authors

Dong Wang

Joel Griffitts

Colby Starker

Elena Fedorova

Erik Limpens

Sergey Ivanov

Ton Bisseling

Sharon Long

Affiliations

Soon will be listed here.

Abstract

The nitrogen-fixing symbiosis between Sinorhizobium meliloti and its leguminous host plant Medicago truncatula occurs in a specialized root organ called the nodule. Bacteria that are released into plant cells are surrounded by a unique plant membrane compartment termed a symbiosome. We found that in the symbiosis-defective dnf1 mutant of M. truncatula, bacteroid and symbiosome development are blocked. We identified the DNF1 gene as encoding a subunit of a signal peptidase complex that is highly expressed in nodules. By analyzing data from whole-genome expression analysis, we propose that correct symbiosome development in M. truncatula requires the orderly secretion of protein constituents through coordinated up-regulation of a nodule-specific pathway exemplified by DNF1.

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