Comparison of the BD GeneOhm Methicillin-resistant Staphylococcus Aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from Intensive Care Unit Patients

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2010 Feb 26

PMID 20181916

Citations 17

Authors

James W Snyder

Gina K Munier

Charles L Johnson

Affiliations

Soon will be listed here.

Abstract

This study compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) real-time PCR assay to culture by the use of BBL CHROMagar MRSA for the detection of MRSA in 627 nasal surveillance specimens collected from intensive care unit (ICU) patients. The PCR assay had a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 96.7%, 70.3%, and 100%, respectively. Nine of 19 false-positive PCR specimens grew methicillin-susceptible S. aureus (MSSA) from broth enrichment culture, of which two demonstrated evidence of mecA gene dropout. Compared to culture by the use of BBL CHROMagar MRSA, the BD GeneOhm MRSA PCR assay demonstrated sensitivity and specificity above 95% for the detection of MRSA nasal colonization and provided shorter turnaround time in generating positive and negative final results.

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References

de San N, Denis O, Gasasira M, De Mendonca R, Nonhoff C, Struelens M . Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens. J Clin Microbiol. 2007; 45(4):1098-101. PMC: 1865822. DOI: 10.1128/JCM.02208-06. View

Warren D, Liao R, Merz L, Eveland M, Dunne Jr W . Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Microbiol. 2004; 42(12):5578-81. PMC: 535250. DOI: 10.1128/JCM.42.12.5578-5581.2004. View

Rossney A, Herra C, Brennan G, Morgan P, OConnell B . Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the GeneXpert real-time PCR platform for rapid detection of MRSA from screening specimens. J Clin Microbiol. 2008; 46(10):3285-90. PMC: 2566096. DOI: 10.1128/JCM.02487-07. View

Kuehnert M, Hill H, Kupronis B, Tokars J, Solomon S, Jernigan D . Methicillin-resistant-Staphylococcus aureus hospitalizations, United States. Emerg Infect Dis. 2005; 11(6):868-72. PMC: 3367609. DOI: 10.3201/eid1106.040831. View

Kelley P, Grabsch E, Howden B, Gao W, Grayson M . Comparison of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay, BD GeneOhm MRSA assay, and culture for detection of nasal and cutaneous groin colonization by MRSA. J Clin Microbiol. 2009; 47(11):3769-72. PMC: 2772639. DOI: 10.1128/JCM.00303-09. View

Peterson L, Hacek D, Robicsek A . 5 Million Lives Campaign. Case study: an MRSA intervention at Evanston Northwestern Healthcare. Jt Comm J Qual Patient Saf. 2008; 33(12):732-8. DOI: 10.1016/s1553-7250(07)33088-2. View

Muto C, Jernigan J, Ostrowsky B, Richet H, Jarvis W, Boyce J . SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus. Infect Control Hosp Epidemiol. 2003; 24(5):362-86. DOI: 10.1086/502213. View

Engemann J, Carmeli Y, Cosgrove S, Fowler V, Bronstein M, Trivette S . Adverse clinical and economic outcomes attributable to methicillin resistance among patients with Staphylococcus aureus surgical site infection. Clin Infect Dis. 2003; 36(5):592-8. DOI: 10.1086/367653. View

Bishop E, Grabsch E, Ballard S, Mayall B, Xie S, Martin R . Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2006; 44(8):2904-8. PMC: 1594615. DOI: 10.1128/JCM.02211-05. View

10.

Farr B . Prevention and control of methicillin-resistant Staphylococcus aureus infections. Curr Opin Infect Dis. 2004; 17(4):317-22. DOI: 10.1097/01.qco.0000136926.52673.cd. View

11.

Cosgrove S, Sakoulas G, Perencevich E, Schwaber M, Karchmer A, Carmeli Y . Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin Infect Dis. 2002; 36(1):53-9. DOI: 10.1086/345476. View

12.

Flayhart D, Hindler J, Bruckner D, Hall G, Shrestha R, Vogel S . Multicenter evaluation of BBL CHROMagar MRSA medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. J Clin Microbiol. 2005; 43(11):5536-40. PMC: 1287825. DOI: 10.1128/JCM.43.11.5536-5540.2005. View

13.

Nguyen Van J, Kitzis M, Ly A, Chalfine A, Carlet J, Ben Ali A . [Detection of nasal colonization methicillin-resistant Staphylococcus aureus: a prospective study comparing real-time genic amplification assay vs selective chromogenic media]. Pathol Biol (Paris). 2006; 54(5):285-92. DOI: 10.1016/j.patbio.2006.01.001. View

14.

Mertz D, Frei R, Jaussi B, Tietz A, Stebler C, Fluckiger U . Throat swabs are necessary to reliably detect carriers of Staphylococcus aureus. Clin Infect Dis. 2007; 45(4):475-7. DOI: 10.1086/520016. View

15.

Paule S, Hacek D, Kufner B, Truchon K, Thomson Jr R, Kaul K . Performance of the BD GeneOhm methicillin-resistant Staphylococcus aureus test before and during high-volume clinical use. J Clin Microbiol. 2007; 45(9):2993-8. PMC: 2045305. DOI: 10.1128/JCM.00670-07. View

16.

Drews S, Willey B, Kreiswirth N, Wang M, Ianes T, Mitchell J . Verification of the IDI-MRSA assay for detecting methicillin-resistant Staphylococcus aureus in diverse specimen types in a core clinical laboratory setting. J Clin Microbiol. 2006; 44(10):3794-6. PMC: 1594777. DOI: 10.1128/JCM.01509-06. View

17.

Currie A, Davis L, Odrobina E, Waldman S, White D, Tomassi J . Sensitivities of nasal and rectal swabs for detection of methicillin-resistant Staphylococcus aureus colonization in an active surveillance program. J Clin Microbiol. 2008; 46(9):3101-3. PMC: 2546770. DOI: 10.1128/JCM.00848-08. View

18.

Wolk D, Marx J, Dominguez L, Driscoll D, Schifman R . Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities. J Clin Microbiol. 2009; 47(12):3933-6. PMC: 2786641. DOI: 10.1128/JCM.00601-09. View

19.

Blot S, Vandewoude K, Hoste E, Colardyn F . Outcome and attributable mortality in critically Ill patients with bacteremia involving methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Arch Intern Med. 2002; 162(19):2229-35. DOI: 10.1001/archinte.162.19.2229. View

20.

Desjardins M, Guibord C, Lalonde B, Toye B, Ramotar K . Evaluation of the IDI-MRSA assay for detection of methicillin-resistant staphylococcus aureus from nasal and rectal specimens pooled in a selective broth. J Clin Microbiol. 2006; 44(4):1219-23. PMC: 1448652. DOI: 10.1128/JCM.44.4.1219-1223.2006. View