Expression of Helios, an Ikaros Transcription Factor Family Member, Differentiates Thymic-derived from Peripherally Induced Foxp3+ T Regulatory Cells

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 2010 Feb 26

PMID 20181882

Citations 718

Authors

Angela M Thornton

Patricia E Korty

Dat Q Tran

Elizabeth A Wohlfert

Patrick E Murray

Yasmine Belkaid

Ethan M Shevach

Affiliations

Soon will be listed here.

Abstract

Helios, a member of the Ikaros transcription factor family, is preferentially expressed at the mRNA level by regulatory T cells (Treg cells). We evaluated Helios protein expression using a newly generated mAb and demonstrated that it is expressed in all thymocytes at the double negative 2 stage of thymic development. Although Helios was expressed by 100% of CD4(+)CD8(-)Foxp3(+) thymocytes, its expression in peripheral lymphoid tissues was restricted to a subpopulation ( approximately 70%) of Foxp3(+) T cells in mice and humans. Neither mouse nor human naive T cells induced to express Foxp3 in vitro by TCR stimulation in the presence of TGF-beta expressed Helios. Ag-specific Foxp3(+) T cells induced in vivo by Ag feeding also failed to express Helios. Collectively, these results demonstrate that Helios is potentially a specific marker of thymic-derived Treg cells and raises the possibility that a significant percentage of Foxp3(+) Treg cells are generated extrathymically.

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