Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver Cells As a Source of Liver Stem/progenitor Cells

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2010 Feb 25

PMID 20180050

Citations 11

Authors

Wei-hui Liu

Ren Li

Ke-Feng Dou

Affiliations

Soon will be listed here.

Abstract

Although the stem cells are commonly isolated by FACS or MACS, they are very expensive and these is no specific marker for liver stem/progentior cells (LSPCs). This paper applied a convenient and efficient method to enrich LSPCs. The fetal liver cells (FLCs) were firstly enriched by Percoll discontinuous gradient centrifugation (PDGC) from the rat fetal liver. Then the FLCs in culture were purified to be homogeneous in size by differential trypsinization and differential adherence (DTDA). Flow cytometric analysis revealed more than half of the purified FLCs expressed alternative markers of LSPCs (CD117, c-Met, Sca-1, CD90, CD49f and CD133). In other words, the purified FLCs were heterogeneous. Therefore, they were sequentially layered into six fractions by Percoll continuous gradient centrifugation (PCGC). Both CD133 and CD49f expressed decreasingly from fraction 1 to 6. In fraction 1 and 2, about 85% FLCs expressed CD133, which were revealed to be LSPCs by high expressions of AFP and CK-19, low expressions of G-6-P and ALB. To conclude, the purity of CD133(+) LSPCs enriched by combination of PDGC, DTDA and PCGC is close to that obtained by MACS. This study will greatly contribute to two important biological aspects: liver stem cells isolation and liver cell therapy.

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References

Nierhoff D, LeVoci L, Schulte S, Goeser T, Rogler L, Shafritz D . New cell surface markers for murine fetal hepatic stem cells identified through high density complementary DNA microarrays. Hepatology. 2007; 46(2):535-47. DOI: 10.1002/hep.21721. View

Morrison S, Spradling A . Stem cells and niches: mechanisms that promote stem cell maintenance throughout life. Cell. 2008; 132(4):598-611. PMC: 4505728. DOI: 10.1016/j.cell.2008.01.038. View

Oertel M, Menthena A, Chen Y, Teisner B, Jensen C, Shafritz D . Purification of fetal liver stem/progenitor cells containing all the repopulation potential for normal adult rat liver. Gastroenterology. 2008; 134(3):823-32. DOI: 10.1053/j.gastro.2008.01.007. View

Fujikawa T, Hirose T, Fujii H, Oe S, Yasuchika K, Azuma H . Purification of adult hepatic progenitor cells using green fluorescent protein (GFP)-transgenic mice and fluorescence-activated cell sorting. J Hepatol. 2003; 39(2):162-70. DOI: 10.1016/s0168-8278(03)00237-x. View

Sandhu J, Petkov P, DABEVA M, Shafritz D . Stem cell properties and repopulation of the rat liver by fetal liver epithelial progenitor cells. Am J Pathol. 2001; 159(4):1323-34. PMC: 1850488. DOI: 10.1016/S0002-9440(10)62519-9. View

Rao M, Khan A, Parveen N, Habeeb M, Habibullah C, Pande G . Characterization of hepatic progenitors from human fetal liver during second trimester. World J Gastroenterol. 2008; 14(37):5730-7. PMC: 2748210. DOI: 10.3748/wjg.14.5730. View

Corcelle V, Stieger B, Gjinovci A, Wollheim C, Gauthier B . Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins. Exp Cell Res. 2006; 312(15):2826-36. DOI: 10.1016/j.yexcr.2006.05.007. View

Zheng Y, Taniguchi H . Diversity of hepatic stem cells in the fetal and adult liver. Semin Liver Dis. 2004; 23(4):337-48. DOI: 10.1055/s-2004-815557. View

Shmelkov S, Meeus S, Moussazadeh N, Kermani P, Rashbaum W, Rabbany S . Cytokine preconditioning promotes codifferentiation of human fetal liver CD133+ stem cells into angiomyogenic tissue. Circulation. 2005; 111(9):1175-83. DOI: 10.1161/01.CIR.0000157155.44008.0F. View

10.

Beaudry P, Hida Y, Udagawa T, Alwayn I, Greene A, Arsenault D . Endothelial progenitor cells contribute to accelerated liver regeneration. J Pediatr Surg. 2007; 42(7):1190-8. DOI: 10.1016/j.jpedsurg.2007.02.034. View

11.

Schmelzer E, Zhang L, Bruce A, Wauthier E, Ludlow J, Yao H . Human hepatic stem cells from fetal and postnatal donors. J Exp Med. 2007; 204(8):1973-87. PMC: 2118675. DOI: 10.1084/jem.20061603. View

12.

Rajvanshi P, Kerr A, Bhargava K, Burk R, Gupta S . Studies of liver repopulation using the dipeptidyl peptidase IV-deficient rat and other rodent recipients: cell size and structure relationships regulate capacity for increased transplanted hepatocyte mass in the liver lobule. Hepatology. 1996; 23(3):482-96. DOI: 10.1002/hep.510230313. View

13.

Koenig S, Krause P, Drabent B, Schaeffner I, Christ B, Schwartz P . The expression of mesenchymal, neural and haematopoietic stem cell markers in adult hepatocytes proliferating in vitro. J Hepatol. 2006; 44(6):1115-24. DOI: 10.1016/j.jhep.2005.09.016. View

14.

Yamazaki S, Miki K, Takayama T, Hasegawa K, Sata M, Midorikawa Y . Hepatic gene induction in murine bone marrow after hepatectomy. J Hepatol. 2005; 44(2):325-33. DOI: 10.1016/j.jhep.2005.07.025. View

15.

Tamaki S, Eckert K, He D, Sutton R, Doshe M, Jain G . Engraftment of sorted/expanded human central nervous system stem cells from fetal brain. J Neurosci Res. 2002; 69(6):976-86. DOI: 10.1002/jnr.10412. View

16.

Perez C, Moreno S, Summerfield A, Domenech N, Alvarez B, Correa C . Characterisation of porcine bone marrow progenitor cells identified by the anti-c-kit (CD117) monoclonal antibody 2B8/BM. J Immunol Methods. 2007; 321(1-2):70-9. DOI: 10.1016/j.jim.2007.01.003. View

17.

Kordes C, Sawitza I, Muller-Marbach A, Ale-Agha N, Keitel V, Klonowski-Stumpe H . CD133+ hepatic stellate cells are progenitor cells. Biochem Biophys Res Commun. 2006; 352(2):410-7. DOI: 10.1016/j.bbrc.2006.11.029. View

18.

Yu S, Zhang J, Zhao C, Zhang H, Xu Q . Isolation and characterization of the CD133+ precursors from the ventricular zone of human fetal brain by magnetic affinity cell sorting. Biotechnol Lett. 2004; 26(14):1131-6. DOI: 10.1023/B:BILE.0000035484.64499.ac. View

19.

Suetsugu A, Nagaki M, Aoki H, Motohashi T, Kunisada T, Moriwaki H . Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem Biophys Res Commun. 2006; 351(4):820-4. DOI: 10.1016/j.bbrc.2006.10.128. View

20.

Tsuchiya A, Heike T, Fujino H, Shiota M, Umeda K, Yoshimoto M . Long-term extensive expansion of mouse hepatic stem/progenitor cells in a novel serum-free culture system. Gastroenterology. 2005; 128(7):2089-104. DOI: 10.1053/j.gastro.2005.03.030. View