Simultaneous Suppression of Three Genes Related to Brassinosteroid (BR) Biosynthesis Altered Campesterol and BR Contents, and Led to a Dwarf Phenotype in Arabidopsis Thaliana

Overview

Journal Plant Cell Rep

Publisher Springer

Date 2010 Feb 20

PMID 20169349

Citations 5

Authors

Ho Yong Chung

Shozo Fujioka

Sunghwa Choe

Soyoun Lee

Youn Hyung Lee

Nam In Baek

In Sik Chung

Affiliations

Soon will be listed here.

Abstract

We generated transgenic lines of Arabidopsis thaliana with an RNA interference construct that expressed hairpin double-stranded RNA for DET2:DWF4:SMT2 to induce sequence-specific RNA silencing. In transgenic plants, expressions of DET2, DWF4, and SMT2 were simultaneously reduced, and the campesterol content was increased by up to 420% compared to the level in the wild-type plant. Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates. Transgenic plants harboring the RNA interference construct displayed a semi-dwarf phenotype due to altered development. Our findings indicate that redesigning of plant architecture is possible through simultaneous suppression of multiple genes involved in BR biosynthesis.

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