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The Relevance of Ice Crystal Formation for the Cryopreservation of Tissues and Organs

Overview
Journal Cryobiology
Publisher Elsevier
Specialty Biology
Date 2010 Feb 18
PMID 20159009
Citations 45
Authors
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Abstract

This paper discusses the role of ice crystal formation in causing or contributing to the difficulties that have been encountered in attempts to develop effective methods for the cryopreservation of some tissues and all organs. It is shown that extracellular ice can be severely damaging but also that cells in situ in tissues can behave quite differently from similar cells in a suspension with respect to intracellular freezing. It is concluded that techniques that avoid the formation of ice altogether are most likely to yield effective methods for the cryopreservation of recalcitrant tissues and vascularised organs.

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