Evaluation of the Anti-endotoxin Effects of Polymyxin B in a Feline Model of Endotoxemia

Overview

Journal J Feline Med Surg

Specialty Veterinary Medicine

Date 2010 Feb 17

PMID 20156699

Citations 5

Authors

Claire R Sharp

Amy E DeClue

Carol E Haak

Allison R Honaker

Carol R Reinero

Affiliations

Soon will be listed here.

Abstract

Directed, effective therapies for feline sepsis are needed to reduce the high morbidity and mortality associated with this disease. We investigated the anti-endotoxin effects of polymyxin B (PMB) in a blinded, placebo controlled fashion, both ex vivo in a feline whole blood culture system and in vivo, using a low-dose endotoxin infusion in cats. Serial measures of systemic inflammation, and hemodynamic stability, were compared between groups. Ex vivo, PMB significantly decreased lipopolysaccharide-induced tumor necrosis factor (TNF) production from whole blood. PMB (1mg/kg over 30min) demonstrated anti-endotoxin effects in vivo, including decreased peak plasma TNF activity (P<0.001) and increased white blood cell count (P=0.019), with no adverse effects. Given the apparent safety and anti-endotoxin effects of PMB in this endotoxemia model, a carefully designed, randomized, blinded, placebo controlled clinical trial evaluating the use of PMB in naturally occurring Gram-negative feline sepsis should be considered.

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