Uncoupling of Expression of an Intronic MicroRNA and Its Myosin Host Gene by Exon Skipping

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2010 Feb 16

PMID 20154144

Citations 76

Authors

Matthew L Bell

Massimo Buvoli

Leslie A Leinwand

Affiliations

Soon will be listed here.

Abstract

The ancient MYH7b gene, expressed in striated muscle and brain, encodes a sarcomeric myosin and the intronic microRNA miR-499. We find that skipping of an exon introduces a premature termination codon in the transcript that downregulates MYH7b protein production without affecting microRNA expression. Among other genes, endogenous miR-499 targets the 3' untranslated region of the transcription factor Sox6, which in turn acts as a repressor of MYH7b transcriptional activity. Thus, concerted transcription and alternative splicing uncouple the level of expression of MYH7b and miR-499 when their coexpression is not required.

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