Grape Seed Proanthocyanidin Suppression of Breast Cell Carcinogenesis Induced by Chronic Exposure to Combined 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and Benzo[a]pyrene

Overview

Journal Mol Carcinog

Specialties Molecular Biology
Oncology

Date 2010 Feb 11

PMID 20146248

Citations 17

Authors

Xiaoyu Song

Nalin Siriwardhana

Kusum Rathore

Degui Lin

Hwa-Chain Robert Wang

Affiliations

Soon will be listed here.

Abstract

Breast cancer is the most common type of cancer among women in northern America and northern Europe; dietary prevention is a cost-efficient strategy to reduce the risk of this disease. To identify dietary components for the prevention of human breast cancer associated with long-term exposure to environmental carcinogens, we studied the activity of grape seed proanthocyanidin extract (GSPE) in suppression of cellular carcinogenesis induced by repeated exposures to low doses of environmental carcinogens. We used combined carcinogens 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and benzo[a]pyrene (B[a]P), at picomolar concentrations, to repeatedly treat noncancerous, human breast epithelial MCF10A cells to induce cellular acquisition of cancer-related properties of reduced dependence on growth factors, anchorage-independent growth, and acinar-conformational disruption. Using these properties as biological target endpoints, we verified the ability of GSPE to suppress combined NNK- and B[a]P-induced precancerous cellular carcinogenesis and identified the minimal, noncytotoxic concentration of GSPE required for suppressing precancerous cellular carcinogenesis. We also identified that hydroxysteroid-11-beta-dehydrogenase 2 (HSD11B2) may play a role in NNK- and B[a]P-induced precancerous cellular carcinogenesis, and its expression may act as a molecular target endpoint in GSPE's suppression of precancerous cellular carcinogenesis. And, the ability of GSPE to reduce gene expression of cytochrome-P450 enzymes CYP1A1 and CYP1B1, which can bioactivate NNK and B[a]P, possibly contributes to the preventive mechanism for GSPE in suppression of precancerous cellular carcinogenesis. Our model system with biological and molecular target endpoints verified the value of GSPE for the prevention of human breast cell carcinogenesis induced by repeated exposures to low doses of multiple environmental carcinogens.

Citing Articles

The Synergistic Effect of Proanthocyanidin and HDAC Inhibitor Inhibit Breast Cancer Cell Growth and Promote Apoptosis.

Wang T, Xu S, Fan Y, Wu J, Wang Z, Chen Y Int J Mol Sci. 2023; 24(13).

PMID: 37445654 PMC: 10341808. DOI: 10.3390/ijms241310476.

Role of Polyphenol-Derived Phenolic Acid in Mitigation of Inflammasome-Mediated Anxiety and Depression.

Iban-Arias R, Sebastian-Valverde M, Wu H, Lyu W, Wu Q, Simon J Biomedicines. 2022; 10(6).

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Grape seed proanthocyanidin extract induces apoptosis of HL-60/ADR cells via the Bax/Bcl-2 caspase-3/9 signaling pathway.

Lin K, Zhao W, Huang S, Li H Transl Cancer Res. 2022; 10(9):3939-3947.

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Carvedilol suppresses malignant proliferation of mammary epithelial cells through inhibition of the ROS‑mediated PI3K/AKT signaling pathway.

Ma Z, Liu X, Zhang Q, Yu Z, Gao D Oncol Rep. 2018; 41(2):811-818.

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Comparison of the Anti-inflammatory Effects of Proanthocyanidin, Quercetin, and Damnacanthal on Benzo(a)pyrene Exposed A549 Alveolar Cell Line.

Gunay E, Celik S, Sarinc-Ulasli S, Ozyurek A, Hazman O, Gunay S Inflammation. 2016; 39(2):744-51.

PMID: 26747272 DOI: 10.1007/s10753-015-0301-3.

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