Suspension Matrices for Improved Schwann-cell Survival After Implantation into the Injured Rat Spinal Cord

Overview

Journal J Neurotrauma

Publisher Mary Ann Liebert

Specialties Emergency Medicine
Neurology

Date 2010 Feb 11

PMID 20144012

Citations 30

Authors

Vivek Patel

Gravil Joseph

Amit Patel

Samik Patel

Devin Bustin

David Mawson

Luis M Tuesta

Rocio Puentes

Mousumi Ghosh

Damien D Pearse

Affiliations

Soon will be listed here.

Abstract

Trauma to the spinal cord produces endogenously irreversible tissue and functional loss, requiring the application of therapeutic approaches to achieve meaningful restoration. Cellular strategies, in particular Schwann-cell implantation, have shown promise in overcoming many of the obstacles facing successful repair of the injured spinal cord. Here, we show that the implantation of Schwann cells as cell suspensions with in-situ gelling laminin:collagen matrices after spinal-cord contusion significantly enhances long-term cell survival but not proliferation, as well as improves graft vascularization and the degree of axonal in-growth over the standard implantation vehicle, minimal media. The use of a matrix to suspend cells prior to implantation should be an important consideration for achieving improved survival and effectiveness of cellular therapies for future clinical application.

Citing Articles

Schwann Cell-Derived Exosomal Vesicles: A Promising Therapy for the Injured Spinal Cord.

Ghosh M, Pearse D Int J Mol Sci. 2023; 24(24.

PMID: 38139147 PMC: 10743801. DOI: 10.3390/ijms242417317.

Injectable hydrogels in central nervous system: Unique and novel platforms for promoting extracellular matrix remodeling and tissue engineering.

Hasanzadeh E, Seifalian A, Mellati A, Saremi J, Asadpour S, Enderami S Mater Today Bio. 2023; 20:100614.

PMID: 37008830 PMC: 10050787. DOI: 10.1016/j.mtbio.2023.100614.

Temporary induction of hypoxic adaptations by preconditioning fails to enhance Schwann cell transplant survival after spinal cord injury.

David B, Curtin J, Brown J, Scorpio K, Kandaswamy V, Coutts D Glia. 2022; 71(3):648-666.

PMID: 36565279 PMC: 11848738. DOI: 10.1002/glia.24302.

Biomaterial-Based Schwann Cell Transplantation and Schwann Cell-Derived Biomaterials for Nerve Regeneration.

Rao Z, Lin Z, Song P, Quan D, Bai Y Front Cell Neurosci. 2022; 16:926222.

PMID: 35836742 PMC: 9273721. DOI: 10.3389/fncel.2022.926222.

Recombinant Adenoviruses for Delivery of Therapeutics Following Spinal Cord Injury.

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