DNA Flow Cytometry of Follicular Non-Hodgkin's Lymphoma

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1991 Mar 1

PMID 2013622

Citations 5

Authors

J C Macartney

R S Camplejohn

R Morris

K Hollowood

D Clarke

A Timothy

Affiliations

Soon will be listed here.

Abstract

S-phase fraction, an index of cellular proliferation, and DNA ploidy were measured by DNA flow cytometry in a retrospective study of lymph node biopsy specimens from 83 cases (before treatment) of follicular non-Hodgkin's lymphoma, Working Formulation categories B and C. The correlations between these measures and survival, clinical stage, symptoms and histopathological factors were investigated. Aneuploidy was rare (n = 16) and had no effect on length of survival or transformation to high grade lymphoma. The overall mean S-phase fraction was 3.6%; for the whole series increasing S-phase fraction was associated with decreased survival. A high S-phase fraction (more than 5%) in initial biopsy specimens was also associated with an increased risk of subsequent high grade transformation at relapse. There was no difference between the survival or proliferative activity of tumours composed of mainly small cleaved cells compared with those composed of mixed small and large cells. There was no difference in survival or proliferative activity between tumours showing a pure follicular growth pattern and those with a mixed follicular and diffuse growth pattern. Multifactorial analysis showed that an S-phase fraction of more than 5% and B symptoms were the most important factors determining survival in these follicular non-Hodgkin's lymphomas.

Citing Articles

Clinical and molecular prognostic factors in follicular lymphoma.

Davies A Curr Oncol Rep. 2006; 8(5):359-67.

PMID: 16901397 DOI: 10.1007/s11912-006-0059-8.

Tumour cell proliferation, but not apoptosis, predicts survival in B-cell non-Hodgkin's lymphomas.

Stokke T, Smeland E, Kvaloy S, Holte H Br J Cancer. 1998; 77(11):1839-41.

PMID: 9667655 PMC: 2150310. DOI: 10.1038/bjc.1998.305.

Proliferation and apoptosis in malignant and normal cells in B-cell non-Hodgkin's lymphomas.

Stokke T, Holte H, Smedshammer L, Smeland E, Kaalhus O, Steen H Br J Cancer. 1998; 77(11):1832-8.

PMID: 9667654 PMC: 2150350. DOI: 10.1038/bjc.1998.304.

Nucleolar organiser regions and survival in patients with non-Hodgkin's lymphomas classified by the working formulation.

Jakic-Razumovic J, Tentor D, Petrovecki M, Radman I J Clin Pathol. 1993; 46(10):943-7.

PMID: 8227414 PMC: 501624. DOI: 10.1136/jcp.46.10.943.

Correlations between apoptotic and proliferative indices in malignant non-Hodgkin's lymphomas.

Leoncini L, Del Vecchio M, Megha T, Barbini P, Galieni P, Pileri S Am J Pathol. 1993; 142(3):755-63.

PMID: 7681257 PMC: 1886795.

References

Peckham M, Cooper E . The pattern of cell growth in reticulum cell sarcoma and lymphosarcoma. Eur J Cancer (1965). 1970; 6(5):453-63. DOI: 10.1016/0014-2964(70)90045-9. View

Macartney J, Camplejohn R . DNA flow cytometry of non-Hodgkin's lymphomas. Eur J Cancer. 1990; 26(5):635-7. DOI: 10.1016/0277-5379(90)90095-b. View

Bennett M . Sclerosis in non-Hodgkin's lymphomata. Br J Cancer Suppl. 1975; 2:44-52. PMC: 2149572. View

Peto R, Pike M, Armitage P, Breslow N, Cox D, Howard S . Design and analysis of randomized clinical trials requiring prolonged observation of each patient. II. analysis and examples. Br J Cancer. 1977; 35(1):1-39. PMC: 2025310. DOI: 10.1038/bjc.1977.1. View

Stein R, Cousar J, FLEXNER J, Graber S, McKee L, Krantz S . Malignant lymphomas of follicular center cell origin in man. III. Prognostic features. Cancer. 1979; 44(6):2236-43. DOI: 10.1002/1097-0142(197912)44:6<2236::aid-cncr2820440635>3.0.co;2-e. View

Ezdinli E, Costello W, Icli F, LENHARD R, Johnson G, Silverstein M . Nodular mixed lymphocytic-histiocytic lymphoma (NM): response and survival. Eastern Cooperative Oncology Group. Cancer. 1980; 45(2):261-7. DOI: 10.1002/1097-0142(19800115)45:2<261::aid-cncr2820450210>3.0.co;2-w. View

Costa A, Bonadonna G, Villa E, Valagussa P, Silvestrini R . Labeling index as a prognostic marker in non-Hodgkin's lymphomas. J Natl Cancer Inst. 1981; 66(1):1-5. View

Hedley D, Friedlander M, TAYLOR I, Rugg C, Musgrove E . Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983; 31(11):1333-5. DOI: 10.1177/31.11.6619538. View

Paryani S, Hoppe R, Cox R, Colby T, Rosenberg S, Kaplan H . Analysis of non-Hodgkin's lymphomas with nodular and favorable histologies, stages I and II. Cancer. 1983; 52(12):2300-7. DOI: 10.1002/1097-0142(19831215)52:12<2300::aid-cncr2820521224>3.0.co;2-y. View

10.

Gospodarowicz M, Bush R, Brown T, Chua T . Prognostic factors in nodular lymphomas: a multivariate analysis based on the Princess Margaret Hospital experience. Int J Radiat Oncol Biol Phys. 1984; 10(4):489-97. DOI: 10.1016/0360-3016(84)90028-2. View

11.

Brittinger G, Bartels H, Common H, Duhmke E, Fulle H, Gunzer U . Clinical and prognostic relevance of the Kiel classification of non-Hodgkin lymphomas results of a prospective multicenter study by the Kiel Lymphoma Study Group. Hematol Oncol. 1984; 2(3):269-306. DOI: 10.1002/hon.2900020306. View

12.

Camplejohn R, Macartney J . Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma. J Clin Pathol. 1985; 38(10):1096-9. PMC: 499448. DOI: 10.1136/jcp.38.10.1096. View

13.

Macartney J, Camplejohn R, Alder J, Stone M, Powell G . Prognostic importance of DNA flow cytometry in non-Hodgkin's lymphomas. J Clin Pathol. 1986; 39(5):542-6. PMC: 499917. DOI: 10.1136/jcp.39.5.542. View

14.

Christensson B, Tribukait B, Linder I, Ullman B, Biberfeld P . Cell proliferation and DNA content in non-Hodgkin's lymphoma. Flow cytometry in relation to lymphoma classification. Cancer. 1986; 58(6):1295-304. DOI: 10.1002/1097-0142(19860915)58:6<1295::aid-cncr2820580620>3.0.co;2-h. View

15.

Gallagher C, Gregory W, Jones A, Stansfeld A, Richards M, Dhaliwal H . Follicular lymphoma: prognostic factors for response and survival. J Clin Oncol. 1986; 4(10):1470-80. DOI: 10.1200/JCO.1986.4.10.1470. View

16.

Akerman M, Brandt L, Johnson A, Olsson H . Mitotic activity in non-Hodgkin's lymphoma. Relation to the Kiel classification and to prognosis. Br J Cancer. 1987; 55(2):219-23. PMC: 2002081. DOI: 10.1038/bjc.1987.41. View

17.

ELLISON D, Nathwani B, Metter G, Turner R, Burke J, Miller T . Mitotic counts in follicular lymphomas. Hum Pathol. 1987; 18(5):502-5. DOI: 10.1016/s0046-8177(87)80035-7. View

18.

Hall P, Richards M, Gregory W, DArdenne A, Lister T, Stansfeld A . The prognostic value of Ki67 immunostaining in non-Hodgkin's lymphoma. J Pathol. 1988; 154(3):223-35. DOI: 10.1002/path.1711540305. View

19.

Griffin N, Howard M, Quirke P, OBrien C, Child J, Bird C . Prognostic indicators in centroblastic-centrocytic lymphoma. J Clin Pathol. 1988; 41(8):866-70. PMC: 1141617. DOI: 10.1136/jcp.41.8.866. View

20.

Lehtinen T, Aine R, Lehtinen M, Kallioniemi O, Leino T, Hakala T . Flow cytometric DNA analysis of 199 histologically favourable or unfavourable non-Hodgkin lymphomas. J Pathol. 1989; 157(1):27-36. DOI: 10.1002/path.1711570105. View