The Expression of Wnt4 is Regulated by Estrogen Via an Estrogen Receptor Alpha-dependent Pathway in Rat Pituitary Growth Hormone-producing Cells

Overview

Journal Acta Histochem Cytochem

Specialty Biochemistry

Date 2010 Feb 4

PMID 20126574

Citations 16

Authors

Takashi Miyakoshi

Hanako Kajiya

Katsuhiro Miyajima

Mao Takei

Maya Tobita

Susumu Takekoshi

Robert Yoshiyuki Osamura

Affiliations

Soon will be listed here.

Abstract

Wnt signaling is important in many aspects of cell biology and development. In the mouse female reproductive tract, Wnt4, Wnt5a, and Wnt7a show differential expression during the estrus cycle, suggesting that they participate in female reproductive physiology. Although the pituitary is a major gland regulating reproduction, the molecular mechanism of Wnt signaling here is unclear. We elucidated the subcellular distribution of Wnt4 in the pituitary of estrogen-treated ovariectomized female rats. Expression of Wnt4 mRNA increased dramatically, particularly in proestrus compared with estrus and metestrus. Wnt4 protein was observed in the cytoplasm of almost all growth hormone (GH)-producing cells and in only a few thyroid-stimulating hormone beta (TSHbeta)-producing cells. In rat GH-producing pituitary tumor (MtT/S) cells, estrogen-induced expression of Wnt4 mRNA was completely inhibited by estrogen receptor antagonist ICI 182,780 in vitro. Thus, rat pituitary GH cells synthesize Wnt4 and this is induced by estrogen mediated via an estrogen receptor alpha-dependent pathway.

Citing Articles

Transcriptomic Analysis of Pubertal and Adult Virgin Mouse Mammary Epithelial and Stromal Cell Populations.

Heijmans N, Wiese K, Jonkers J, van Amerongen R J Mammary Gland Biol Neoplasia. 2024; 29(1):13.

PMID: 38916673 PMC: 11199289. DOI: 10.1007/s10911-024-09565-1.

Oestrogen-induced epithelial-mesenchymal transition (EMT) in endometriosis: Aetiology of vaginal agenesis in Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome.

Lih Yuan T, Sulaiman N, Nur Azurah A, Maarof M, Razali R, Yazid M Front Physiol. 2022; 13:937988.

PMID: 36582359 PMC: 9793092. DOI: 10.3389/fphys.2022.937988.

Wnt/β-catenin signaling pathway in uterine leiomyoma: role in tumor biology and targeting opportunities.

El Sabeh M, Saha S, Afrin S, Islam M, Borahay M Mol Cell Biochem. 2021; 476(9):3513-3536.

PMID: 33999334 PMC: 9235413. DOI: 10.1007/s11010-021-04174-6.

Exogenous Oestrogen Impacts Cell Fate Decision in the Developing Gonads: A Potential Cause of Declining Human Reproductive Health.

Stewart M, Mattiske D, Pask A Int J Mol Sci. 2020; 21(21).

PMID: 33171657 PMC: 7664701. DOI: 10.3390/ijms21218377.

Estrogen suppresses SOX9 and activates markers of female development in a human testis-derived cell line.

Stewart M, Mattiske D, Pask A BMC Mol Cell Biol. 2020; 21(1):66.

PMID: 32933467 PMC: 7493336. DOI: 10.1186/s12860-020-00307-9.

References

Hou X, Tan Y, Li M, Dey S, Das S . Canonical Wnt signaling is critical to estrogen-mediated uterine growth. Mol Endocrinol. 2004; 18(12):3035-49. PMC: 4280566. DOI: 10.1210/me.2004-0259. View

Vainio S, Heikkila M, Kispert A, Chin N, McMahon A . Female development in mammals is regulated by Wnt-4 signalling. Nature. 1999; 397(6718):405-9. DOI: 10.1038/17068. View

Li S, Crenshaw 3rd E, Rawson E, SIMMONS D, Swanson L, Rosenfeld M . Dwarf locus mutants lacking three pituitary cell types result from mutations in the POU-domain gene pit-1. Nature. 1990; 347(6293):528-33. DOI: 10.1038/347528a0. View

Takada R, Satomi Y, Kurata T, Ueno N, Norioka S, Kondoh H . Monounsaturated fatty acid modification of Wnt protein: its role in Wnt secretion. Dev Cell. 2006; 11(6):791-801. DOI: 10.1016/j.devcel.2006.10.003. View

Dang V, Nguyen T, Lee G, Choi K, Jeung E . In vitro exposure to xenoestrogens induces growth hormone transcription and release via estrogen receptor-dependent pathways in rat pituitary GH3 cells. Steroids. 2009; 74(8):707-14. DOI: 10.1016/j.steroids.2009.03.002. View

Mericskay M, Kitajewski J, Sassoon D . Wnt5a is required for proper epithelial-mesenchymal interactions in the uterus. Development. 2004; 131(9):2061-72. DOI: 10.1242/dev.01090. View

Egashira N, Minematsu T, Miyai S, Takekoshi S, Camper S, Osamura R . Pituitary changes in Prop1 transgenic mice: hormone producing tumors and signet-ring type gonadotropes. Acta Histochem Cytochem. 2008; 41(3):47-57. PMC: 2447862. DOI: 10.1267/ahc.08007. View

Shaikh A . Estrone and estradiol levels in the ovarian venous blood from rats during the estrous cycle and pregnancy. Biol Reprod. 1971; 5(3):297-307. DOI: 10.1093/biolreprod/5.3.297. View

Miyakoshi T, Miyajima K, Takekoshi S, Osamura R . The influence of endocrine disrupting chemicals on the proliferation of ERalpha knockdown-human breast cancer cell line MCF-7; new attempts by RNAi technology. Acta Histochem Cytochem. 2009; 42(2):23-8. PMC: 2685020. DOI: 10.1267/ahc.08036. View

10.

Jeays-Ward K, Dandonneau M, Swain A . Wnt4 is required for proper male as well as female sexual development. Dev Biol. 2004; 276(2):431-40. DOI: 10.1016/j.ydbio.2004.08.049. View

11.

Mueller S . Xenoestrogens: mechanisms of action and detection methods. Anal Bioanal Chem. 2003; 378(3):582-7. DOI: 10.1007/s00216-003-2238-x. View

12.

Brisken C, Heineman A, Chavarria T, Elenbaas B, Tan J, Dey S . Essential function of Wnt-4 in mammary gland development downstream of progesterone signaling. Genes Dev. 2000; 14(6):650-4. PMC: 316462. View

13.

Shaikh A, Shaikh S . Adrenal and ovarian steroid secretion in the rat estrous cycle temporally related to gonadotropins and steroid levels found in peripheral plasma. Endocrinology. 1975; 96(1):37-44. DOI: 10.1210/endo-96-1-37. View

14.

Stark K, Vainio S, Vassileva G, McMahon A . Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. Nature. 1994; 372(6507):679-83. DOI: 10.1038/372679a0. View

15.

Matthews J, Gustafsson J . Estrogen signaling: a subtle balance between ER alpha and ER beta. Mol Interv. 2004; 3(5):281-92. DOI: 10.1124/mi.3.5.281. View

16.

Kispert A, Vainio S, McMahon A . Wnt-4 is a mesenchymal signal for epithelial transformation of metanephric mesenchyme in the developing kidney. Development. 1998; 125(21):4225-34. DOI: 10.1242/dev.125.21.4225. View

17.

Childs G, Iruthayanathan M, Akhter N, Unabia G, Whitehead-Johnson B . Bipotential effects of estrogen on growth hormone synthesis and storage in vitro. Endocrinology. 2004; 146(4):1780-8. PMC: 1751515. DOI: 10.1210/en.2004-1111. View

18.

Katoh M . WNT/PCP signaling pathway and human cancer (review). Oncol Rep. 2005; 14(6):1583-8. View

19.

Scully K, Gleiberman A, Lindzey J, Lubahn D, Korach K, Rosenfeld M . Role of estrogen receptor-alpha in the anterior pituitary gland. Mol Endocrinol. 1997; 11(6):674-81. DOI: 10.1210/mend.11.6.0019. View

20.

Elston M, Gill A, Conaglen J, Clarkson A, Shaw J, Law A . Wnt pathway inhibitors are strongly down-regulated in pituitary tumors. Endocrinology. 2007; 149(3):1235-42. DOI: 10.1210/en.2007-0542. View