Glycoprotein Clearance is Rapid and Suppressed by Mannan in Chicken Embryos

Overview

Journal J Physiol Biochem

Specialties Biochemistry
Physiology

Date 2010 Feb 2

PMID 20119818

Authors

G Wu

Z Li

Affiliations

Soon will be listed here.

Abstract

Carbohydrates are thought to function as tags that mark circulatory glycoproteins for rapid clearance. Scavenger endothelial cells (SECs) play the primary role in clearing glycoproteins via receptor-mediated endocytosis in adult animals. We found that horseradish peroxidase (HRP), a glycoprotein, was removed quickly, mostly by receptor mediation from the chicken embryo circulation, but bovine serum albumin was not. The half-life of HRP in the circulation varied with the embryo stage and fell rapidly from 0.73 h at embryonic day 4 (E4) to 0.23 h at E5, with no great difference among stages after E5. HRP clearance was far slower at E3.5 than at E5, but was obviously suppressed by mannan. These results imply that the function of clearing glycoprotein or waste macromolecules from the circulation via receptor-mediated endocytosis appears early in the embryo.

References

Taylor P, Gordon S, Martinez-Pomares L . The mannose receptor: linking homeostasis and immunity through sugar recognition. Trends Immunol. 2005; 26(2):104-10. DOI: 10.1016/j.it.2004.12.001. View

Kawasaki T, ASHWELL G . Isolation and characterization of an avian hepatic binding protein specific for N-acetylglucosamine-terminated glycoproteins. J Biol Chem. 1977; 252(18):6536-43. View

Sugimoto Y, Sanuki S, Ohsako S, Higashimoto Y, Kondo M, Kurawaki J . Ovalbumin in developing chicken eggs migrates from egg white to embryonic organs while changing its conformation and thermal stability. J Biol Chem. 1999; 274(16):11030-7. DOI: 10.1074/jbc.274.16.11030. View

Goldstein J, Ho Y, Basu S, Brown M . Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc Natl Acad Sci U S A. 1979; 76(1):333-7. PMC: 382933. DOI: 10.1073/pnas.76.1.333. View

ASHWELL G, Morell A . The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol Relat Areas Mol Biol. 1974; 41(0):99-128. DOI: 10.1002/9780470122860.ch3. View

Smedsrod B . Clearance function of scavenger endothelial cells. Comp Hepatol. 2004; 3 Suppl 1:S22. PMC: 2409441. DOI: 10.1186/1476-5926-2-S1-S22. View

Lee S, Evers S, Roeder D, Parlow A, Risteli J, Risteli L . Mannose receptor-mediated regulation of serum glycoprotein homeostasis. Science. 2002; 295(5561):1898-901. DOI: 10.1126/science.1069540. View

Seternes T, Sorensen K, Smedsrod B . Scavenger endothelial cells of vertebrates: a nonperipheral leukocyte system for high-capacity elimination of waste macromolecules. Proc Natl Acad Sci U S A. 2002; 99(11):7594-7. PMC: 124295. DOI: 10.1073/pnas.102173299. View

Mego J, Bertini F, McQUEEN J . The use of formaldehyde-treated 131-I-albumin in the study of digestive vacuoles and some properties of these particles from mouse liver. J Cell Biol. 1967; 32(3):699-707. PMC: 2107274. DOI: 10.1083/jcb.32.3.699. View

10.

Sallusto F, Cella M, Danieli C, Lanzavecchia A . Dendritic cells use macropinocytosis and the mannose receptor to concentrate macromolecules in the major histocompatibility complex class II compartment: downregulation by cytokines and bacterial products. J Exp Med. 1995; 182(2):389-400. PMC: 2192110. DOI: 10.1084/jem.182.2.389. View

11.

Malovic I, Sorensen K, Elvevold K, Nedredal G, Paulsen S, Erofeev A . The mannose receptor on murine liver sinusoidal endothelial cells is the main denatured collagen clearance receptor. Hepatology. 2007; 45(6):1454-61. DOI: 10.1002/hep.21639. View

12.

Dini L, Mattioli P . Carbohydrate recognition systems in avian sinusoidal liver cells. Biosci Rep. 1990; 10(1):37-46. DOI: 10.1007/BF01116849. View

13.

Rockey D, Bissell D . Noninvasive measures of liver fibrosis. Hepatology. 2006; 43(2 Suppl 1):S113-20. DOI: 10.1002/hep.21046. View