Physiologically Relevant Small Phosphodonors Link Metabolism to Signal Transduction

Overview

Journal Curr Opin Microbiol

Specialty Microbiology

Date 2010 Feb 2

PMID 20117041

Citations 76

Authors

Alan J Wolfe

Affiliations

Soon will be listed here.

Abstract

Recent reports support the long-standing hypothesis that acetyl phosphate, a physiologically relevant small molecule, can serve as a phosphoryl donor to a subset of two-component response regulators that regulate diverse cellular processes. Since acetyl phosphate is a central metabolite, this ability would link nutritional status to global signaling. This review will first introduce acetyl phosphate and its pathway. It will then summarize the most compelling evidence supporting the hypothesis and list predicted properties of an acetyl phosphate-sensitive pathway. Next, it will describe emerging evidence that acetyl phosphate and/or its pathway can influence diverse cellular processes across a broad spectrum of bacteria. Finally, the review will explore the possibility that other metabolites can function in a capacity similar to acetyl phosphate.

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References

Hutchings M, Hong H, Buttner M . The vancomycin resistance VanRS two-component signal transduction system of Streptomyces coelicolor. Mol Microbiol. 2006; 59(3):923-35. DOI: 10.1111/j.1365-2958.2005.04953.x. View

Haldimann A, Fisher S, Daniels L, WALSH C, Wanner B . Transcriptional regulation of the Enterococcus faecium BM4147 vancomycin resistance gene cluster by the VanS-VanR two-component regulatory system in Escherichia coli K-12. J Bacteriol. 1997; 179(18):5903-13. PMC: 179483. DOI: 10.1128/jb.179.18.5903-5913.1997. View

Feng J, Atkinson M, McCleary W, Stock J, Wanner B, Ninfa A . Role of phosphorylated metabolic intermediates in the regulation of glutamine synthetase synthesis in Escherichia coli. J Bacteriol. 1992; 174(19):6061-70. PMC: 207671. DOI: 10.1128/jb.174.19.6061-6070.1992. View

Danese P, Silhavy T . CpxP, a stress-combative member of the Cpx regulon. J Bacteriol. 1998; 180(4):831-9. PMC: 106961. DOI: 10.1128/JB.180.4.831-839.1998. View

Wolfe A, Chang D, Walker J, Seitz-Partridge J, Vidaurri M, Lange C . Evidence that acetyl phosphate functions as a global signal during biofilm development. Mol Microbiol. 2003; 48(4):977-88. DOI: 10.1046/j.1365-2958.2003.03457.x. View

Carlsson K, Liu J, Edqvist P, Francis M . Influence of the Cpx extracytoplasmic-stress-responsive pathway on Yersinia sp.-eukaryotic cell contact. Infect Immun. 2007; 75(9):4386-99. PMC: 1951158. DOI: 10.1128/IAI.01450-06. View

Kim Y, Brinsmade S, Yang Z, Escalante-Semerena J, Fierer J . Mutation of phosphotransacetylase but not isocitrate lyase reduces the virulence of Salmonella enterica serovar Typhimurium in mice. Infect Immun. 2006; 74(4):2498-502. PMC: 1418904. DOI: 10.1128/IAI.74.4.2498-2502.2006. View

Kobayashi K . Gradual activation of the response regulator DegU controls serial expression of genes for flagellum formation and biofilm formation in Bacillus subtilis. Mol Microbiol. 2007; 66(2):395-409. DOI: 10.1111/j.1365-2958.2007.05923.x. View

Yu B, Kim J, Moon J, Ryu S, Pan J . The diversity of lysine-acetylated proteins in Escherichia coli. J Microbiol Biotechnol. 2008; 18(9):1529-36. View

10.

Fredericks C, Shibata S, Aizawa S, Reimann S, Wolfe A . Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcs phosphorelay. Mol Microbiol. 2006; 61(3):734-47. DOI: 10.1111/j.1365-2958.2006.05260.x. View

11.

El-Mansi M . Flux to acetate and lactate excretions in industrial fermentations: physiological and biochemical implications. J Ind Microbiol Biotechnol. 2004; 31(7):295-300. DOI: 10.1007/s10295-004-0149-2. View

12.

Mizrahi I, Biran D, Ron E . Involvement of the Pta-AckA pathway in protein folding and aggregation. Res Microbiol. 2008; 160(1):80-4. DOI: 10.1016/j.resmic.2008.10.007. View

13.

Wang Q, Zhao Y, McClelland M, Harshey R . The RcsCDB signaling system and swarming motility in Salmonella enterica serovar typhimurium: dual regulation of flagellar and SPI-2 virulence genes. J Bacteriol. 2007; 189(23):8447-57. PMC: 2168921. DOI: 10.1128/JB.01198-07. View

14.

Arthur M, Depardieu F, Gerbaud G, Galimand M, Leclercq R, Courvalin P . The VanS sensor negatively controls VanR-mediated transcriptional activation of glycopeptide resistance genes of Tn1546 and related elements in the absence of induction. J Bacteriol. 1997; 179(1):97-106. PMC: 178666. DOI: 10.1128/jb.179.1.97-106.1997. View

15.

Ramos-Montanez S, Tsui H, Wayne K, Morris J, Peters L, Zhang F . Polymorphism and regulation of the spxB (pyruvate oxidase) virulence factor gene by a CBS-HotDog domain protein (SpxR) in serotype 2 Streptococcus pneumoniae. Mol Microbiol. 2008; 67(4):729-46. DOI: 10.1111/j.1365-2958.2007.06082.x. View

16.

McCleary W, Stock J, Ninfa A . Is acetyl phosphate a global signal in Escherichia coli?. J Bacteriol. 1993; 175(10):2793-8. PMC: 204593. DOI: 10.1128/jb.175.10.2793-2798.1993. View

17.

Martin W, Russell M . On the origin of biochemistry at an alkaline hydrothermal vent. Philos Trans R Soc Lond B Biol Sci. 2007; 362(1486):1887-925. PMC: 2442388. DOI: 10.1098/rstb.2006.1881. View

18.

Ninfa A, Jiang P, Atkinson M, Peliska J . Integration of antagonistic signals in the regulation of nitrogen assimilation in Escherichia coli. Curr Top Cell Regul. 2000; 36:31-75. DOI: 10.1016/s0070-2137(01)80002-9. View

19.

Voet J, Voet D . We have a new publisher: John Wiley & Sons. Biochem Mol Biol Educ. 2011; 35(1):1. DOI: 10.1002/bmb.20. View

20.

Gueriri I, Bay S, Dubrac S, Cyncynatus C, Msadek T . The Pta-AckA pathway controlling acetyl phosphate levels and the phosphorylation state of the DegU orphan response regulator both play a role in regulating Listeria monocytogenes motility and chemotaxis. Mol Microbiol. 2008; 70(6):1342-57. DOI: 10.1111/j.1365-2958.2008.06496.x. View