Cloning and Sequence Determination of the Schizosaccharomyces Pombe Rpb1 Gene Encoding the Largest Subunit of RNA Polymerase II

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1991 Feb 11

PMID 2011520

Citations 19

Authors

Y Azuma

M Yamagishi

R UESHIMA

A Ishihama

Affiliations

Soon will be listed here.

Abstract

The gene, rpb1, encoding the largest subunit of RNA polymerase II has been cloned from Schizosaccharomyces pombe using the corresponding gene, RPB1, of Saccharomyces cerevisiae as a cross-hybridization probe. We have determined the complete sequence of this gene, and parts of PCR-amplified rpb1 cDNA. The predicted coding sequence, interrupted by six introns, encodes a polypeptide of 1,752 amino acid residues in length with a molecular weight of 194 kilodaltons. This polypeptide contains eight conserved structural domains characteristic of the largest subunit of RNA polymerases from other eukaryotes and, in addition, 29 repetitions of the C-terminal heptapeptide found in all the eukaryotic RNA polymerase II largest subunits so far examined.

Citing Articles

Cdk9 regulates a promoter-proximal checkpoint to modulate RNA polymerase II elongation rate in fission yeast.

Booth G, Parua P, Sanso M, Fisher R, Lis J Nat Commun. 2018; 9(1):543.

PMID: 29416031 PMC: 5803247. DOI: 10.1038/s41467-018-03006-4.

Punctuation and syntax of the RNA polymerase II CTD code in fission yeast.

Schwer B, Sanchez A, Shuman S Proc Natl Acad Sci U S A. 2012; 109(44):18024-9.

PMID: 23071310 PMC: 3497752. DOI: 10.1073/pnas.1208995109.

Separable functions of the fission yeast Spt5 carboxyl-terminal domain (CTD) in capping enzyme binding and transcription elongation overlap with those of the RNA polymerase II CTD.

Schneider S, Pei Y, Shuman S, Schwer B Mol Cell Biol. 2010; 30(10):2353-64.

PMID: 20231361 PMC: 2863715. DOI: 10.1128/MCB.00116-10.

RNA polymerase II transcription apparatus in Schizosaccharomyces pombe.

Mitsuzawa H, Ishihama A Curr Genet. 2003; 44(6):287-94.

PMID: 14574615 DOI: 10.1007/s00294-003-0446-8.

Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II.

Kimura M, Suzuki H, Ishihama A Mol Cell Biol. 2002; 22(5):1577-88.

PMID: 11839823 PMC: 134712. DOI: 10.1128/MCB.22.5.1577-1588.2002.

References

Kock J, Evers R, Cornelissen A . Structure and sequence of the gene for the largest subunit of trypanosomal RNA polymerase III. Nucleic Acids Res. 1988; 16(18):8753-72. PMC: 338633. DOI: 10.1093/nar/16.18.8753. View

Helmann J, CHAMBERLIN M . Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988; 57:839-72. DOI: 10.1146/annurev.bi.57.070188.004203. View

Geiduschek E, Tocchini-Valentini G . Transcription by RNA polymerase III. Annu Rev Biochem. 1988; 57:873-914. DOI: 10.1146/annurev.bi.57.070188.004301. View

Wittekind M, Dodd J, Vu L, Kolb J, Buhler J, Sentenac A . Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Mol Cell Biol. 1988; 8(10):3997-4008. PMC: 365468. DOI: 10.1128/mcb.8.10.3997-4008.1988. View

Evers R, Hammer A, Kock J, Jess W, Borst P, Memet S . Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domain. Cell. 1989; 56(4):585-97. DOI: 10.1016/0092-8674(89)90581-3. View

Kozak M . The scanning model for translation: an update. J Cell Biol. 1989; 108(2):229-41. PMC: 2115416. DOI: 10.1083/jcb.108.2.229. View

Hirano T, Konoha G, Toda T, Yanagida M . Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus. J Cell Biol. 1989; 108(2):243-53. PMC: 2115420. DOI: 10.1083/jcb.108.2.243. View

Smith J, Levin J, Ingles C, Agabian N . In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure. Cell. 1989; 56(5):815-27. DOI: 10.1016/0092-8674(89)90686-7. View

Allison L, Ingles C . Mutations in RNA polymerase II enhance or suppress mutations in GAL4. Proc Natl Acad Sci U S A. 1989; 86(8):2794-8. PMC: 287005. DOI: 10.1073/pnas.86.8.2794. View

10.

Jokerst R, Weeks J, Zehring W, Greenleaf A . Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila. Mol Gen Genet. 1989; 215(2):266-75. DOI: 10.1007/BF00339727. View

11.

Yamagishi M, Nomura M . Cloning and sequence determination of the gene encoding the largest subunit of the fission yeast Schizosaccharomyces pombe RNA polymerase I. Gene. 1988; 74(2):503-15. DOI: 10.1016/0378-1119(88)90183-7. View

12.

Jess W, Hammer A, Cornelissen A . Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei. FEBS Lett. 1989; 249(1):123-8. DOI: 10.1016/0014-5793(89)80029-8. View

13.

Ishihama A . Promoter selectivity of prokaryotic RNA polymerases. Trends Genet. 1988; 4(10):282-6. DOI: 10.1016/0168-9525(88)90170-9. View

14.

Woychik N, Young R . RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Mol Cell Biol. 1989; 9(7):2854-9. PMC: 362751. DOI: 10.1128/mcb.9.7.2854-2859.1989. View

15.

Smith J, Levin J, Agabian N . Molecular characterization of the Trypanosoma brucei RNA polymerase I and III largest subunit genes. J Biol Chem. 1989; 264(30):18091-9. View

16.

Bird D, Riddle D . Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II. Mol Cell Biol. 1989; 9(10):4119-30. PMC: 362490. DOI: 10.1128/mcb.9.10.4119-4130.1989. View

17.

Kolodziej P, Young R . RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. Mol Cell Biol. 1989; 9(12):5387-94. PMC: 363706. DOI: 10.1128/mcb.9.12.5387-5394.1989. View

18.

Li W, Bzik D, Gu H, Tanaka M, Fox B, Inselburg J . An enlarged largest subunit of Plasmodium falciparum RNA polymerase II defines conserved and variable RNA polymerase domains. Nucleic Acids Res. 1989; 17(23):9621-36. PMC: 335202. DOI: 10.1093/nar/17.23.9621. View

19.

Hein J . Unified approach to alignment and phylogenies. Methods Enzymol. 1990; 183:626-45. DOI: 10.1016/0076-6879(90)83041-7. View

20.

Carothers A, Urlaub G, Mucha J, Grunberger D, Chasin L . Point mutation analysis in a mammalian gene: rapid preparation of total RNA, PCR amplification of cDNA, and Taq sequencing by a novel method. Biotechniques. 1989; 7(5):494-6, 498-9. View