Two Chitinase-like Proteins Abundantly Accumulated in Latex of Mulberry Show Insecticidal Activity

Overview

Journal BMC Biochem

Publisher Biomed Central

Specialty Biochemistry

Date 2010 Jan 30

PMID 20109180

Citations 13

Authors

Sakihito Kitajima

Kaeko Kamei

Shigeru Taketani

Masamitsu Yamaguchi

Fumiko Kawai

Aino Komatsu

Yoshihiro Inukai

Affiliations

Soon will be listed here.

Abstract

Background: Plant latex is the cytoplasm of highly specialized cells known as laticifers, and is thought to have a critical role in defense against herbivorous insects. Proteins abundantly accumulated in latex might therefore be involved in the defense system.

Results: We purified latex abundant protein a and b (LA-a and LA-b) from mulberry (Morus sp.) and analyzed their properties. LA-a and LA-b have molecular masses of approximately 50 and 46 kDa, respectively, and are abundant in the soluble fraction of latex. Western blotting analysis suggested that they share sequence similarity with each other. The sequences of LA-a and LA-b, as determined by Edman degradation, showed chitin-binding domains of plant chitinases at the N termini. These proteins showed small but significant chitinase and chitosanase activities. Lectin RCA120 indicated that, unlike common plant chitinases, LA-a and LA-b are glycosylated. LA-a and LA-b showed insecticidal activities when fed to larvae of the model insect Drosophila melanogaster.

Conclusions: Our results suggest that the two LA proteins have a crucial role in defense against herbivorous insects, possibly by hydrolyzing their chitin.

Citing Articles

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Expression of a gene for an MLX56 defense protein derived from mulberry latex confers strong resistance against a broad range of insect pests on transgenic tomato lines.

Murata M, Konno K, Wasano N, Mochizuki A, Mitsuhara I PLoS One. 2021; 16(1):e0239958.

PMID: 33428626 PMC: 7799757. DOI: 10.1371/journal.pone.0239958.

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