Rapid Diagnosis of Azole-resistant Aspergillosis by Direct PCR Using Tissue Specimens

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2010 Jan 29

PMID 20107096

Citations 26

Authors

Jan W M van der Linden

Eveline Snelders

Jan P Arends

Simon M Daenen

Willem J G Melchers

Paul E Verweij

Affiliations

Soon will be listed here.

Abstract

We report the use of PCR techniques on a formalin-fixed and paraffin-embedded tissue specimen for direct detection of one dominant azole resistance mechanism in a case of disseminated invasive aspergillosis. Rapid detection of mutations associated with azole resistance directly in tissue significantly reduces diagnostic delay.

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