Precise Excision of IS5 from the Intergenic Region Between the FucPIK and the FucAO Operons and Mutational Control of FucPIK Operon Expression in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 2010 Jan 26

PMID 20097855

Citations 13

Authors

Zhongge Zhang

Ming Ren Yen

Milton H Saier Jr

Affiliations

Soon will be listed here.

Abstract

Excision of transposable genetic elements from host DNA occurs at low frequencies and is usually imprecise. A common insertion sequence element in Escherichia coli, IS5, has been shown to provide various benefits to its host by inserting into specific sites. Precise excision of this element had not previously been demonstrated. Using a unique system, the fucose (fuc) regulon, in which IS5 insertion and excision result in two distinct selectable phenotypes, we have demonstrated that IS5 can precisely excise from its insertion site, restoring the wild-type phenotype. In addition to precise excision, several "suppressor" insertion, deletion, and point mutations restore the wild-type Fuc(+) phenotype to various degrees without IS5 excision. The possible bases for these observations are discussed.

Citing Articles

Transcriptional mechanism by which IS5 activates the fucAO operon in Escherichia coli.

Zhou H, Zhang Z, Velo J, Huo J, Smith S, Ho A Nucleic Acids Res. 2025; 53(5).

PMID: 40066878 PMC: 11894529. DOI: 10.1093/nar/gkaf172.

Investigating How Genomic Contexts Impact IS5 Transposition Within the Genome.

Onstead J, Zhang Z, Huo J, Ord J, Smith S, Saier Jr M Microorganisms. 2025; 12(12.

PMID: 39770802 PMC: 11677980. DOI: 10.3390/microorganisms12122600.

The effect of DNA-binding proteins on insertion sequence element transposition upstream of the operon in .

Kopkowski P, Zhang Z, Saier Jr M Front Microbiol. 2024; 15:1388522.

PMID: 38666260 PMC: 11043490. DOI: 10.3389/fmicb.2024.1388522.

The Transposition of Insertion Sequences in Sigma-Factor- and LysR-Deficient Mutants of .

Park J, Lee S, Shin E, Abdi Nansa S, Lee S Microorganisms. 2024; 12(2).

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Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of the Operon.

Tran D, Zhang Z, Lam K, Saier Jr M Int J Mol Sci. 2022; 23(18).

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