Simian Virus 40-specific Proteins in the Membranes of Simian Virus 40-transformed Hamster and Mouse Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Nov 1

PMID 200945

Citations 5

Authors

R Schmidt-Ullrich

W S Thompson

P S Lin

D F Wallach

Affiliations

Soon will be listed here.

Abstract

Membranes of simian virus 40-transformed hamster lymphocytes and phagocytes, as well as of transformed mouse fibroblasts, contain two classes of antigenic virus-specific protein. The isoelectric points of these proteins, as defined by isoelectric focusing/immune electrophoresis are at pH 4.5 and 4.7. The molecular weights of the pI 4.5 and pI 4.7 components, determined by isoelectric focusing/dodecyl sulfate polyacrylamide electrophoresis, lie near 58,000 and 90,000-110,000, respectively. The pI 4.5 and pI 4.7 proteins are tentatively identified with the surface (transplantation) and U antigens, respectively.

Citing Articles

Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells.

McCormick F, Harlow E J Virol. 1980; 34(1):213-24.

PMID: 6246255 PMC: 288687. DOI: 10.1128/JVI.34.1.213-224.1980.

Two classes of transformation-deficient, immortalization-positive simian virus 40 mutants constructed by making three-base insertions in the T antigen gene.

Sugano S, Yamaguchi N J Virol. 1984; 52(3):884-91.

PMID: 6092718 PMC: 254609. DOI: 10.1128/JVI.52.3.884-891.1984.

Cell-free synthesis of simian virus 40 T-antigens.

Paucha E, Harvey R, Smith A J Virol. 1978; 28(1):154-70.

PMID: 212600 PMC: 354255. DOI: 10.1128/JVI.28.1.154-170.1978.

Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.

Smith A, Smith R, Paucha E J Virol. 1978; 28(1):140-53.

PMID: 212599 PMC: 354254. DOI: 10.1128/JVI.28.1.140-153.1978.

Plasmodium knowlesi-induced antigens in membranes of parasitized rhesus monkey erythrocytes.

Schmidt-Ullrich R, Wallach D Proc Natl Acad Sci U S A. 1978; 75(10):4949-53.

PMID: 105359 PMC: 336239. DOI: 10.1073/pnas.75.10.4949.

References

Lin P, Schmidt-Ullrich R, Wallach D . Transformation by simian virus 40 induces virus-specific, related antigens in the surface membrane and nuclear envelope. Proc Natl Acad Sci U S A. 1977; 74(6):2495-9. PMC: 432199. DOI: 10.1073/pnas.74.6.2495. View

Schmidt-Ullrich R, Thompson W, Wallach D . Antigenic distinctions of glycoproteins in plasma and mitochondrial membranes of lymphoid cells neoplastically transformed by simian virus 40. Proc Natl Acad Sci U S A. 1977; 74(2):643-7. PMC: 392348. DOI: 10.1073/pnas.74.2.643. View

ROBB J . Identification of simian virus 40 tumor and U antigens. Proc Natl Acad Sci U S A. 1977; 74(2):447-51. PMC: 392306. DOI: 10.1073/pnas.74.2.447. View

COE J . Immunoglobulin synthesis by an SV-40-induced hamster lymphoma. Immunology. 1976; 31(3):495-502. PMC: 1445237. View

Chang C, Pancake S, LUBORSKY S, Mora P . Detergent solubilization and partial purification of tumor specific surface and transplantation antigens from SV40-virus-transformed mouse cells. Int J Cancer. 1977; 19(2):258-66. DOI: 10.1002/ijc.2910190216. View

Schmidt-Ullrich R, Wallach D, Davis 2nd F . Membranes of normal hamster lymphocytes and lymphoid cells neoplastically transformed by simian virus 40. II. Plasma membrane proteins analyzed by dodecyl sulfate-polyacrylamide gel electrophoresis and two-dimensional immune electrophoresis. J Natl Cancer Inst. 1976; 57(5):1117-26. DOI: 10.1093/jnci/57.5.1117. View

Schmidt-Ullrich R, Wallach D, Davis 2nd F . Membranes of normal hamster lymphocytes and lymphoid cells neoplastically transformed by simian virus 40. I. High-yield purification of plasma membrane fragments. J Natl Cancer Inst. 1976; 57(5):1107-16. DOI: 10.1093/jnci/57.5.1107. View

Anderson J, Martin R, Chang C, Mora P, Livingston D . Nuclear preparations of SV40-transformed cells contain tumor-specific transplantation antigen activity. Virology. 1977; 76(1):420-5. DOI: 10.1016/0042-6822(77)90314-2. View

LUBORSKY S, Chang C, Pancake S, Mora P . Detergent solubilized and molecular weight estimation of tumor specific surface antigen from SV40 virus transformed cells. Biochem Biophys Res Commun. 1976; 71(4):990-6. DOI: 10.1016/0006-291x(76)90752-x. View

10.

Johansson K, Hjerten S . Localization of the Tween 20-soluble membrane proteins of Acholeplasma laidlawii by crossed immunoelectrophoresis. J Mol Biol. 1974; 86(2):341-8. DOI: 10.1016/0022-2836(74)90023-0. View

11.

Lewis Jr A, Rowe W . Studies on nondefective adenovirus-simian virus 40 hybrid viruses. I. A newly characterized simian virus 40 antigen induced by the Ad2+ND 1 virus. J Virol. 1971; 7(2):189-97. PMC: 356097. DOI: 10.1128/JVI.7.2.189-197.1971. View

12.

COE J, Green I . B-cell origin of hamster lymphoid tumors induced by simian virus 40. J Natl Cancer Inst. 1975; 54(1):269-70. DOI: 10.1093/jnci/54.1.269. View

13.

Schmidt-Ullrich R, Verma S, Wallach D . Anomalous side chain amidation in plasma membrane proteins of simian virus 40-transformed lymphocytes indicated by isoelectric focussing and laser Raman spectroscopy. Biochem Biophys Res Commun. 1975; 67(3):1062-9. DOI: 10.1016/0006-291x(75)90782-2. View

14.

LAURELL C . Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966; 15(1):45-52. DOI: 10.1016/0003-2697(66)90246-6. View