A Simple and Rapid Method for Detection of Goose Parvovirus in the Field by Loop-mediated Isothermal Amplification

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2010 Jan 23

PMID 20092637

Citations 15

Authors

Jinlong Yang

Rui Yang

Anchun Cheng

Mingshu Wang

Lizhi Fu

Songquan Yang

Suhui Zhang

Liu Yang

Zhiyong Xu

Affiliations

Soon will be listed here.

Abstract

Background: Goose parvovirus (GPV) is a Dependovirus associated with latent infection and mortality in geese. Currently, in a worldwide scale, GPV severely affects geese production. The objective of this study is to develop a loop-mediated isothermal amplification (LAMP) method for the sensitive, rapid, and inexpensive detection of GPV in the field.

Results: A set of six specific primers was designed by targeting the GPV VP3 DNA. With Bst DNA polymerase large fragment, the target DNA could be amplified at 65 degrees C as early as 20 min of incubation in a simple water bath. A positive reaction was identified through the detection of the LAMP product by color change visible to the naked eye. The detection limit of the assay was 28 copies/microl of plasmid pVP3, and with equal sensitivity and specificity to fluorescent quantitative real-time PCR (FQ-PCR).

Conclusions: The high sensitivity, specificity, and simplicity, as well as the high throughput, make this method suitable for specific detection of GPV infection in both field conditions and laboratory settings. The utilization of complicated equipment and conduct of technical training on the GPV LAMP were not necessary.

Citing Articles

Rapid on-site differentiation of two invasive whitefly cryptic species using LAMP.

Liu Y, Yang L, Liu Y, Chen Y, Zhang Y, Chu D Sci Rep. 2025; 15(1):5261.

PMID: 39939810 PMC: 11822201. DOI: 10.1038/s41598-025-89429-8.

Rapid and visual detection of an isolated and identified goose parvovirus (GPV) strain by a loop-mediated isothermal amplification assay.

Liu J, Chen Y, Pei Y, Yu Q, Afumba R, Dong H Vet Res Forum. 2023; 14(1):7-12.

PMID: 36816861 PMC: 9906619. DOI: 10.30466/vrf.2021.540351.3246.

Development and Evaluation of NanoPCR for the Detection of Goose Parvovirus.

Ma H, Gao X, Fu J, Xue H, Song Y, Zhu K Vet Sci. 2022; 9(9).

PMID: 36136676 PMC: 9506417. DOI: 10.3390/vetsci9090460.

Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing.

Quyen T, Vinayaka A, Golabi M, Ngoc H, Bang D, Wolff A Front Cell Infect Microbiol. 2022; 12:856553.

PMID: 35521217 PMC: 9065284. DOI: 10.3389/fcimb.2022.856553.

Elaboration of Triplex PCR for Detection of Selected Viral Infections in Waterfowl.

Kozdrun W, Czekaj H, Stys-Fijol N, Piekarska K, Niczyporuk J J Vet Res. 2020; 63(4):503-505.

PMID: 31934659 PMC: 6950434. DOI: 10.2478/jvetres-2019-0074.

References

Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N . Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000; 28(12):E63. PMC: 102748. DOI: 10.1093/nar/28.12.e63. View

Limn C, Yamada T, Nakamura M, Takehara K . Detection of Goose parvovirus genome by polymerase chain reaction: distribution of Goose parvovirus in Muscovy ducklings. Virus Res. 1996; 42(1-2):167-72. DOI: 10.1016/0168-1702(95)01310-5. View

Liu Y, Chuang C, Chen W . In situ reverse-transcription loop-mediated isothermal amplification (in situ RT-LAMP) for detection of Japanese encephalitis viral RNA in host cells. J Clin Virol. 2009; 46(1):49-54. PMC: 7108223. DOI: 10.1016/j.jcv.2009.06.010. View

Kono T, Savan R, Sakai M, Itami T . Detection of white spot syndrome virus in shrimp by loop-mediated isothermal amplification. J Virol Methods. 2003; 115(1):59-65. DOI: 10.1016/j.jviromet.2003.09.015. View

Guo Y, Cheng A, Wang M, Shen C, Jia R, Chen S . Development of TaqMan MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1. Virol J. 2009; 6:71. PMC: 2696427. DOI: 10.1186/1743-422X-6-71. View

Gough D, Ceeraz V, Cox B, Palya V, Mato T . Isolation and identification of goose parvovirus in the UK. Vet Rec. 2005; 156(13):424. DOI: 10.1136/vr.156.13.424. View

Nagamine K, Watanabe K, Ohtsuka K, Hase T, Notomi T . Loop-mediated isothermal amplification reaction using a nondenatured template. Clin Chem. 2001; 47(9):1742-3. View

Yang J, Cheng A, Wang M, Pan K, Li M, Guo Y . Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo. Virol J. 2009; 6:142. PMC: 2751755. DOI: 10.1186/1743-422X-6-142. View

Chen H, Zhang J, Sun D, Ma L, Liu X, Cai X . Development of reverse transcription loop-mediated isothermal amplification for rapid detection of H9 avian influenza virus. J Virol Methods. 2008; 151(2):200-203. DOI: 10.1016/j.jviromet.2008.05.009. View

10.

Brown K, Green S, Young N . Goose parvovirus--an autonomous member of the dependovirus genus?. Virology. 1995; 210(2):283-91. DOI: 10.1006/viro.1995.1345. View

11.

Hara-Kudo Y, Konishi N, Ohtsuka K, Hiramatsu R, Tanaka H, Konuma H . Detection of Verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: a collaborative study. Int J Food Microbiol. 2007; 122(1-2):156-61. DOI: 10.1016/j.ijfoodmicro.2007.11.078. View

12.

Parida M . Rapid and real-time detection technologies for emerging viruses of biomedical importance. J Biosci. 2009; 33(4):617-28. PMC: 7090734. DOI: 10.1007/s12038-008-0079-7. View

13.

Takehara K, Nishio T, Hayashi Y, Kanda J, Sasaki M, Abe N . An outbreak of goose parvovirus infection in Japan. J Vet Med Sci. 1995; 57(4):777-9. DOI: 10.1292/jvms.57.777. View

14.

Savan R, Igarashi A, Matsuoka S, Sakai M . Sensitive and rapid detection of edwardsiellosis in fish by a loop-mediated isothermal amplification method. Appl Environ Microbiol. 2004; 70(1):621-4. PMC: 321279. DOI: 10.1128/AEM.70.1.621-624.2004. View

15.

Mori Y, Nagamine K, Tomita N, Notomi T . Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun. 2001; 289(1):150-4. DOI: 10.1006/bbrc.2001.5921. View

16.

Nagamine K, Hase T, Notomi T . Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes. 2002; 16(3):223-9. DOI: 10.1006/mcpr.2002.0415. View

17.

Holmes J, Jones J, Gough R, Welchman D, Wessels M, Jones E . Goose parvovirus in England and Wales. Vet Rec. 2004; 155(4):127. View

18.

Mao X, Zhou S, Xu D, Gong J, Cui H, Qin Q . Rapid and sensitive detection of Singapore grouper iridovirus by loop-mediated isothermal amplification. J Appl Microbiol. 2008; 105(2):389-97. DOI: 10.1111/j.1365-2672.2008.03761.x. View

19.

Yamazaki W, Seto K, Taguchi M, Ishibashi M, Inoue K . Sensitive and rapid detection of cholera toxin-producing Vibrio cholerae using a loop-mediated isothermal amplification. BMC Microbiol. 2008; 8:94. PMC: 2446398. DOI: 10.1186/1471-2180-8-94. View

20.

Cai T, Lou G, Yang J, Xu D, Meng Z . Development and evaluation of real-time loop-mediated isothermal amplification for hepatitis B virus DNA quantification: a new tool for HBV management. J Clin Virol. 2008; 41(4):270-6. DOI: 10.1016/j.jcv.2007.11.025. View