Development and Characterisation of a Collagen Nano-hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2010 Jan 22

PMID 20091099

Citations 45

Authors

Grainne M Cunniffe

Glenn R Dickson

Sonia Partap

Kenneth T Stanton

Fergal J OBrien

Affiliations

Soon will be listed here.

Abstract

Bone regeneration requires scaffolds that possess suitable mechanical and biological properties. This study sought to develop a novel collagen-nHA biocomposite scaffold via two new methods. Firstly a stable nHA suspension was produced and added to a collagen slurry (suspension method), and secondly, porous collagen scaffolds were immersed in nHA suspension after freeze-drying (immersion method). Significantly stronger constructs were produced using both methods compared to collagen only scaffolds, with a high porosity maintained (>98.9%). It was found that Coll-nHA composite scaffolds produced by the suspension method were up to 18 times stiffer than the collagen control (5.50 +/- 1.70 kPa vs. 0.30 +/- 0.09 kPa). The suspension method was also more reproducible, and the quantity of nHA incorporated could be varied with greater ease than with the immersion technique. In addition, Coll-nHA composites display excellent biological activity, demonstrating their potential as bone graft substitutes in orthopaedic regenerative medicine.

Citing Articles

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References

Harley B, Kim H, Zaman M, V Yannas I, Lauffenburger D, Gibson L . Microarchitecture of three-dimensional scaffolds influences cell migration behavior via junction interactions. Biophys J. 2008; 95(8):4013-24. PMC: 2553126. DOI: 10.1529/biophysj.107.122598. View

Haugh M, Jaasma M, OBrien F . The effect of dehydrothermal treatment on the mechanical and structural properties of collagen-GAG scaffolds. J Biomed Mater Res A. 2008; 89(2):363-9. DOI: 10.1002/jbm.a.31955. View

Visser R, Arrabal P, Becerra J, Rinas U, Cifuentes M . The effect of an rhBMP-2 absorbable collagen sponge-targeted system on bone formation in vivo. Biomaterials. 2009; 30(11):2032-7. DOI: 10.1016/j.biomaterials.2008.12.046. View

OBrien F, Harley B, V Yannas I, Gibson L . Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds. Biomaterials. 2003; 25(6):1077-86. DOI: 10.1016/s0142-9612(03)00630-6. View

OBrien F, Harley B, Waller M, V Yannas I, Gibson L, Prendergast P . The effect of pore size on permeability and cell attachment in collagen scaffolds for tissue engineering. Technol Health Care. 2007; 15(1):3-17. View

Juhasz J, Best S, Brooks R, Kawashita M, Miyata N, Kokubo T . Mechanical properties of glass-ceramic A-W-polyethylene composites: effect of filler content and particle size. Biomaterials. 2003; 25(6):949-55. DOI: 10.1016/j.biomaterials.2003.07.005. View

Hutmacher D . Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000; 21(24):2529-43. DOI: 10.1016/s0142-9612(00)00121-6. View

Cunniffe G, OBrien F, Partap S, Levingstone T, Stanton K, Dickson G . The synthesis and characterization of nanophase hydroxyapatite using a novel dispersant-aided precipitation method. J Biomed Mater Res A. 2010; 95(4):1142-9. DOI: 10.1002/jbm.a.32931. View

Wei G, Ma P . Structure and properties of nano-hydroxyapatite/polymer composite scaffolds for bone tissue engineering. Biomaterials. 2004; 25(19):4749-57. DOI: 10.1016/j.biomaterials.2003.12.005. View

10.

Langer R, Vacanti J . Tissue engineering. Science. 1993; 260(5110):920-6. DOI: 10.1126/science.8493529. View

11.

Heo S, Kim S, Wei J, Hyun Y, Yun H, Kim D . Fabrication and characterization of novel nano- and micro-HA/PCL composite scaffolds using a modified rapid prototyping process. J Biomed Mater Res A. 2008; 89(1):108-16. DOI: 10.1002/jbm.a.31726. View

12.

Harley B, Leung J, Silva E, Gibson L . Mechanical characterization of collagen-glycosaminoglycan scaffolds. Acta Biomater. 2007; 3(4):463-74. DOI: 10.1016/j.actbio.2006.12.009. View

13.

Sun X, Jeng L, Bolliet C, Olsen B, Spector M . Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds. Biomaterials. 2008; 30(6):1222-31. DOI: 10.1016/j.biomaterials.2008.10.020. View

14.

Holladay C, Keeney M, Greiser U, Murphy M, OBrien T, Pandit A . A matrix reservoir for improved control of non-viral gene delivery. J Control Release. 2009; 136(3):220-5. DOI: 10.1016/j.jconrel.2009.02.006. View

15.

Al-Munajjed A, Plunkett N, Gleeson J, Weber T, Jungreuthmayer C, Levingstone T . Development of a biomimetic collagen-hydroxyapatite scaffold for bone tissue engineering using a SBF immersion technique. J Biomed Mater Res B Appl Biomater. 2009; 90(2):584-91. DOI: 10.1002/jbm.b.31320. View

16.

Al-Munajjed A, OBrien F . Influence of a novel calcium-phosphate coating on the mechanical properties of highly porous collagen scaffolds for bone repair. J Mech Behav Biomed Mater. 2009; 2(2):138-46. DOI: 10.1016/j.jmbbm.2008.05.001. View

17.

Rodrigues C, Serricella P, Linhares A, Guerdes R, Borojevic R, Rossi M . Characterization of a bovine collagen-hydroxyapatite composite scaffold for bone tissue engineering. Biomaterials. 2003; 24(27):4987-97. DOI: 10.1016/s0142-9612(03)00410-1. View

18.

Tierney C, Jaasma M, OBrien F . Osteoblast activity on collagen-GAG scaffolds is affected by collagen and GAG concentrations. J Biomed Mater Res A. 2008; 91(1):92-101. DOI: 10.1002/jbm.a.32207. View

19.

Song J, Kim H, Kim H . Electrospun fibrous web of collagen-apatite precipitated nanocomposite for bone regeneration. J Mater Sci Mater Med. 2008; 19(8):2925-32. DOI: 10.1007/s10856-008-3420-7. View

20.

OBrien F, Harley B, Yannas I, Gibson L . The effect of pore size on cell adhesion in collagen-GAG scaffolds. Biomaterials. 2004; 26(4):433-41. DOI: 10.1016/j.biomaterials.2004.02.052. View