Identification of Two Critically Deleted Regions Within Chromosome Segment 7q35-q36 in EVI1 Deregulated Myeloid Leukemia Cell Lines

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2010 Jan 20

PMID 20084277

Citations 10

Authors

An De Weer

Bruce Poppe

Sarah Vergult

Pieter Van Vlierberghe

Marjan Petrick

Robrecht De Bock

Yves Benoit

Lucien Noens

Anne De Paepe

Nadine Van Roy

Bjorn Menten

Frank Speleman

Affiliations

Soon will be listed here.

Abstract

Chromosomal rearrangements involving the EVI1 proto-oncogene are a recurrent finding in myeloid leukemias and are indicative of a poor prognosis. Rearrangements of the EVI1 locus are often associated with monosomy 7 or cytogenetic detectable deletions of part of 7q. As EVI1 overexpression alone is not sufficient to induce leukemia, loss of a 7q tumour suppressor gene might be a required cooperating event. To test this hypothesis, we performed high-resolution array comparative genomic hybridization analysis of twelve EVI1 overexpressing patients and three EVI1 deregulated cell lines to search for 7q submicroscopic deletions. This analysis lead to the delineation of two critical regions, one of 0.39 Mb on 7q35 containing the CNTNAP2 gene and one of 1.33 Mb on chromosome bands 7q35-q36 comprising nine genes in EVI1 deregulated cell lines. These findings open the way to further studies aimed at identifying the culprit EVI1 implicated tumour suppressor genes on 7q.

Citing Articles

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References

Menten B, Pattyn F, De Preter K, Robbrecht P, Michels E, Buysse K . arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays. BMC Bioinformatics. 2005; 6:124. PMC: 1173083. DOI: 10.1186/1471-2105-6-124. View

Cardoso C, Mignon C, Hetet G, Grandchamps B, Fontes M, Colleaux L . The human EZH2 gene: genomic organisation and revised mapping in 7q35 within the critical region for malignant myeloid disorders. Eur J Hum Genet. 2000; 8(3):174-80. DOI: 10.1038/sj.ejhg.5200439. View

Omura N, Li C, Li A, Hong S, Walter K, Jimeno A . Genome-wide profiling of methylated promoters in pancreatic adenocarcinoma. Cancer Biol Ther. 2008; 7(7):1146-56. PMC: 2763640. DOI: 10.4161/cbt.7.7.6208. View

Du Y, Jenkins N, Copeland N . Insertional mutagenesis identifies genes that promote the immortalization of primary bone marrow progenitor cells. Blood. 2005; 106(12):3932-9. PMC: 1895096. DOI: 10.1182/blood-2005-03-1113. View

Dohner K, Brown J, Hehmann U, Hetzel C, Stewart J, Lowther G . Molecular cytogenetic characterization of a critical region in bands 7q35-q36 commonly deleted in malignant myeloid disorders. Blood. 1998; 92(11):4031-5. View

McAvoy S, Ganapathiraju S, Ducharme-Smith A, Pritchett J, Kosari F, Perez D . Non-random inactivation of large common fragile site genes in different cancers. Cytogenet Genome Res. 2007; 118(2-4):260-9. DOI: 10.1159/000108309. View

De Weer A, Speleman F, Cauwelier B, Van Roy N, Yigit N, Verhasselt B . EVI1 overexpression in t(3;17) positive myeloid malignancies results from juxtaposition of EVI1 to the MSI2 locus at 17q22. Haematologica. 2008; 93(12):1903-7. DOI: 10.3324/haematol.13192. View

Hu Z, Ma W, Zaborski M, MacLeod R, Quentmeier H, Drexler H . Establishment and characterization of two novel cytokine-responsive acute myeloid and monocytic leukemia cell lines, MUTZ-2 and MUTZ-3. Leukemia. 1996; 10(6):1025-40. View

Ebert B, Pretz J, Bosco J, Chang C, Tamayo P, Galili N . Identification of RPS14 as a 5q- syndrome gene by RNA interference screen. Nature. 2008; 451(7176):335-9. PMC: 3771855. DOI: 10.1038/nature06494. View

10.

Venkatraman E, Olshen A . A faster circular binary segmentation algorithm for the analysis of array CGH data. Bioinformatics. 2007; 23(6):657-63. DOI: 10.1093/bioinformatics/btl646. View

11.

Asou H, Suzukawa K, Kita K, Nakase K, Ueda H, Morishita K . Establishment of an undifferentiated leukemia cell line (Kasumi-3) with t(3;7)(q27;q22) and activation of the EVI1 gene. Jpn J Cancer Res. 1996; 87(3):269-74. PMC: 5921094. DOI: 10.1111/j.1349-7006.1996.tb00216.x. View

12.

Asnafi V, Buzyn A, Le Noir S, Baleydier F, Simon A, Beldjord K . NOTCH1/FBXW7 mutation identifies a large subgroup with favorable outcome in adult T-cell acute lymphoblastic leukemia (T-ALL): a Group for Research on Adult Acute Lymphoblastic Leukemia (GRAALL) study. Blood. 2008; 113(17):3918-24. DOI: 10.1182/blood-2008-10-184069. View

13.

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

14.

Suzuki E, Handa K, Toledo M, Hakomori S . Sphingosine-dependent apoptosis: a unified concept based on multiple mechanisms operating in concert. Proc Natl Acad Sci U S A. 2004; 101(41):14788-93. PMC: 522055. DOI: 10.1073/pnas.0406536101. View

15.

Calmels B, Ferguson C, Laukkanen M, Adler R, Faulhaber M, Kim H . Recurrent retroviral vector integration at the Mds1/Evi1 locus in nonhuman primate hematopoietic cells. Blood. 2005; 106(7):2530-3. PMC: 1895268. DOI: 10.1182/blood-2005-03-1115. View

16.

Xu Z, Chen L, Leung L, Yen T, Lee C, Chan J . Liver-specific inactivation of the Nrf1 gene in adult mouse leads to nonalcoholic steatohepatitis and hepatic neoplasia. Proc Natl Acad Sci U S A. 2005; 102(11):4120-5. PMC: 554825. DOI: 10.1073/pnas.0500660102. View

17.

Welte K, PLATZER E, Lu L, GABRILOVE J, Levi E, Mertelsmann R . Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor. Proc Natl Acad Sci U S A. 1985; 82(5):1526-30. PMC: 397296. DOI: 10.1073/pnas.82.5.1526. View

18.

Lugthart S, van Drunen E, van Norden Y, van Hoven A, Erpelinck C, Valk P . High EVI1 levels predict adverse outcome in acute myeloid leukemia: prevalence of EVI1 overexpression and chromosome 3q26 abnormalities underestimated. Blood. 2008; 111(8):4329-37. DOI: 10.1182/blood-2007-10-119230. View

19.

Le Beau M, Espinosa 3rd R, Davis E, Eisenbart J, Larson R, Green E . Cytogenetic and molecular delineation of a region of chromosome 7 commonly deleted in malignant myeloid diseases. Blood. 1996; 88(6):1930-5. View

20.

Lopingco M, Perkins A . Molecular analysis of Evi1, a zinc finger oncogene involved in myeloid leukemia. Curr Top Microbiol Immunol. 1996; 211:211-22. DOI: 10.1007/978-3-642-85232-9_21. View