The ND2 Subunit is Labeled by a Photoaffinity Analogue of Asimicin, a Potent Complex I Inhibitor

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2010 Jan 16

PMID 20074573

Citations 21

Authors

Eiko Nakamaru-Ogiso

Hongna Han

Akemi Matsuno-Yagi

Ehud Keinan

Subhash C Sinha

Takao Yagi

Tomoko Ohnishi

Affiliations

Soon will be listed here.

Abstract

NADH:ubiquinone oxidoreductase (complex I) is the entry enzyme of mitochondrial oxidative phosphorylation. To obtain the structural information on inhibitor/quinone binding sites, we synthesized [3H]benzophenone-asimicin ([3H]BPA), a photoaffinity analogue of asimicin, which belongs to the acetogenin family known as the most potent complex I inhibitor. We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS-PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. The cross-linking was blocked by rotenone. This is the first finding that ND2 was photo-crosslinked with a potent complex I inhibitor, suggesting its involvement in the inhibitor/quinone-binding.

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