Human RNA Polymerase I-driven Reverse Genetics for Influenza a Virus in Canine Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2010 Jan 15

PMID 20071567

Citations 8

Authors

Pirada Suphaphiphat

Bjoern Keiner

Heidi Trusheim

Stefania Crotta

Annunziata Barbara Tuccino

Pu Zhang

Philip R Dormitzer

Peter W Mason

Michael Franti

Affiliations

Soon will be listed here.

Abstract

We have established a human RNA polymerase I (pol I)-driven influenza virus reverse genetics (RG) system in the Madin-Darby canine kidney 33016-PF cell line, which is approved for influenza vaccine manufacture. RNA pol I polymerases are generally active only in cells of species closely related to the species of origin of the polymerases. Nevertheless, we show that a nonendogenous RNA pol I promoter drives efficient rescue of influenza A viruses in a canine cell line. Application of this system allows efficient generation of virus strains and presents an alternative approach for influenza vaccine production.

Citing Articles

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Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter.

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References

Hoffmann E, Neumann G, Kawaoka Y, HOBOM G, Webster R . A DNA transfection system for generation of influenza A virus from eight plasmids. Proc Natl Acad Sci U S A. 2000; 97(11):6108-13. PMC: 18566. DOI: 10.1073/pnas.100133697. View

Neumann G, Fujii K, Kino Y, Kawaoka Y . An improved reverse genetics system for influenza A virus generation and its implications for vaccine production. Proc Natl Acad Sci U S A. 2005; 102(46):16825-9. PMC: 1283806. DOI: 10.1073/pnas.0505587102. View

Wang Z, Duke G . Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells. Virol J. 2007; 4:102. PMC: 2241602. DOI: 10.1186/1743-422X-4-102. View

Bell S, Pikaard C, Reeder R, Tjian R . Molecular mechanisms governing species-specific transcription of ribosomal RNA. Cell. 1989; 59(3):489-97. DOI: 10.1016/0092-8674(89)90032-9. View

Lutz A, Dyall J, Olivo P, Pekosz A . Virus-inducible reporter genes as a tool for detecting and quantifying influenza A virus replication. J Virol Methods. 2005; 126(1-2):13-20. PMC: 1698269. DOI: 10.1016/j.jviromet.2005.01.016. View

Bottcher E, Matrosovich T, Beyerle M, Klenk H, Garten W, Matrosovich M . Proteolytic activation of influenza viruses by serine proteases TMPRSS2 and HAT from human airway epithelium. J Virol. 2006; 80(19):9896-8. PMC: 1617224. DOI: 10.1128/JVI.01118-06. View

Bell S, Jantzen H, Tjian R . Assembly of alternative multiprotein complexes directs rRNA promoter selectivity. Genes Dev. 1990; 4(6):943-54. DOI: 10.1101/gad.4.6.943. View

Okuno Y, Tanaka K, Baba K, Maeda A, KUNITA N, Ueda S . Rapid focus reduction neutralization test of influenza A and B viruses in microtiter system. J Clin Microbiol. 1990; 28(6):1308-13. PMC: 267925. DOI: 10.1128/jcm.28.6.1308-1313.1990. View

Heix J, Grummt I . Species specificity of transcription by RNA polymerase I. Curr Opin Genet Dev. 1995; 5(5):652-6. DOI: 10.1016/0959-437x(95)80035-2. View

10.

Nakagawa N, Maeda A, Kase T, Kubota R, Okuno Y . Rapid detection and identification of two lineages of influenza B strains with monoclonal antibodies. J Virol Methods. 1999; 79(1):113-20. DOI: 10.1016/s0166-0934(99)00015-4. View

11.

Fodor E, Devenish L, Engelhardt O, Palese P, Brownlee G, Garcia-Sastre A . Rescue of influenza A virus from recombinant DNA. J Virol. 1999; 73(11):9679-82. PMC: 113010. DOI: 10.1128/JVI.73.11.9679-9682.1999. View

12.

Parodi B, Aresu O, Bini D, Lorenzini R, Schena F, Visconti P . Species identification and confirmation of human and animal cell lines: a PCR-based method. Biotechniques. 2002; 32(2):432-4, 436, 438-40. DOI: 10.2144/02322rr05. View

13.

Cooper J, Sykes G, King S, Cottrill K, Ivanova N, Hanner R . Species identification in cell culture: a two-pronged molecular approach. In Vitro Cell Dev Biol Anim. 2007; 43(10):344-51. DOI: 10.1007/s11626-007-9060-2. View

14.

Ozaki H, Govorkova E, Li C, Xiong X, Webster R, Webby R . Generation of high-yielding influenza A viruses in African green monkey kidney (Vero) cells by reverse genetics. J Virol. 2004; 78(4):1851-7. PMC: 369478. DOI: 10.1128/jvi.78.4.1851-1857.2004. View

15.

Murakami S, Horimoto T, Yamada S, Kakugawa S, Goto H, Kawaoka Y . Establishment of canine RNA polymerase I-driven reverse genetics for influenza A virus: its application for H5N1 vaccine production. J Virol. 2007; 82(3):1605-9. PMC: 2224466. DOI: 10.1128/JVI.01876-07. View