Purification of Structurally Intact Grana from Plants Thylakoids Membranes

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 2010 Jan 13

PMID 20066483

Citations 10

Authors

Tomas Morosinotto

Anna Segalla

Giorgio M Giacometti

Roberto Bassi

Affiliations

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Abstract

Thylakoid membranes in higher plant chloroplasts are composed by two distinct domains: stacked grana and stroma lamellae. We developed a procedure for biochemical isolation of grana membranes using mild detergent to maintain membrane structure. Pigment and polypeptide analyses of membrane preparation showed the preparations were indeed enriched in grana membranes. The method was shown to be effective in four different plant species, although with small changes in detergent concentration. Electron microscopy analyses also showed that the preparation consisted of large membrane patches with roughly round shape and diameter comparable with grana membranes in vivo. Furthermore, protein complexes distribution was shown to be maintained with respect to freeze fracture studies, demonstrating that the protocol was successful in isolating membranes close to their in vivo state.

Citing Articles

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References

Ballottari M, Govoni C, Caffarri S, Morosinotto T . Stoichiometry of LHCI antenna polypeptides and characterization of gap and linker pigments in higher plants Photosystem I. Eur J Biochem. 2004; 271(23-24):4659-65. DOI: 10.1111/j.1432-1033.2004.04426.x. View

van Roon H, van Breemen J, de Weerd F, Dekker J, Boekema E . Solubilization of green plant thylakoid membranes with n-dodecyl-alpha,D-maltoside. Implications for the structural organization of the Photosystem II, Photosystem I, ATP synthase and cytochrome b6 f complexes. Photosynth Res. 2005; 64(2-3):155-66. DOI: 10.1023/A:1006476213540. View

Nelson N, Ben-Shem A . The complex architecture of oxygenic photosynthesis. Nat Rev Mol Cell Biol. 2004; 5(12):971-82. DOI: 10.1038/nrm1525. View

Betterle N, Ballottari M, Zorzan S, De Bianchi S, Cazzaniga S, DallOsto L . Light-induced dissociation of an antenna hetero-oligomer is needed for non-photochemical quenching induction. J Biol Chem. 2009; 284(22):15255-66. PMC: 2685706. DOI: 10.1074/jbc.M808625200. View

Gilmore A, Yamamoto H . Zeaxanthin Formation and Energy-Dependent Fluorescence Quenching in Pea Chloroplasts under Artificially Mediated Linear and Cyclic Electron Transport. Plant Physiol. 1991; 96(2):635-43. PMC: 1080818. DOI: 10.1104/pp.96.2.635. View

Albertsson P . A quantitative model of the domain structure of the photosynthetic membrane. Trends Plant Sci. 2001; 6(8):349-58. DOI: 10.1016/s1360-1385(01)02021-0. View

Vallon O, Bulte L, Dainese P, Olive J, Bassi R, Wollman F . Lateral redistribution of cytochrome b6/f complexes along thylakoid membranes upon state transitions. Proc Natl Acad Sci U S A. 1991; 88(18):8262-6. PMC: 52487. DOI: 10.1073/pnas.88.18.8262. View

Simpson D . Freeze-fracture studies on barley plastid membranes. VI. Location of the P700-chlorophyll a-protein 1. Eur J Cell Biol. 1983; 31(2):305-14. DOI: 10.1007/BF02906493. View

Teardo E, Frare E, Segalla A, De Marco V, Giacometti G, Szabo I . Localization of a putative ClC chloride channel in spinach chloroplasts. FEBS Lett. 2005; 579(22):4991-6. DOI: 10.1016/j.febslet.2005.08.005. View

10.

Morosinotto T, Bassi R, Frigerio S, Finazzi G, Morris E, Barber J . Biochemical and structural analyses of a higher plant photosystem II supercomplex of a photosystem I-less mutant of barley. Consequences of a chronic over-reduction of the plastoquinone pool. FEBS J. 2006; 273(20):4616-30. DOI: 10.1111/j.1742-4658.2006.05465.x. View

11.

Nelson N, Ben-Shem A . The structure of photosystem I and evolution of photosynthesis. Bioessays. 2005; 27(9):914-22. DOI: 10.1002/bies.20278. View

12.

Kirchhoff H, Tremmel I, Haase W, Kubitscheck U . Supramolecular photosystem II organization in grana thylakoid membranes: evidence for a structured arrangement. Biochemistry. 2004; 43(28):9204-13. DOI: 10.1021/bi0494626. View

13.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

14.

Kaftan D, Brumfeld V, Nevo R, Scherz A, Reich Z . From chloroplasts to photosystems: in situ scanning force microscopy on intact thylakoid membranes. EMBO J. 2002; 21(22):6146-53. PMC: 137213. DOI: 10.1093/emboj/cdf624. View

15.

Mustardy L, Garab G . Granum revisited. A three-dimensional model--where things fall into place. Trends Plant Sci. 2003; 8(3):117-22. DOI: 10.1016/S1360-1385(03)00015-3. View

16.

Boekema E, van Breemen J, van Roon H, Dekker J . Arrangement of photosystem II supercomplexes in crystalline macrodomains within the thylakoid membrane of green plant chloroplasts. J Mol Biol. 2000; 301(5):1123-33. DOI: 10.1006/jmbi.2000.4037. View

17.

Albertsson P . The structure and function of the chloroplast photosynthetic membrane - a model for the domain organization. Photosynth Res. 2013; 46(1-2):141-9. DOI: 10.1007/BF00020424. View

18.

Danielsson R, Albertsson P, Mamedov F, Styring S . Quantification of photosystem I and II in different parts of the thylakoid membrane from spinach. Biochim Biophys Acta. 2004; 1608(1):53-61. DOI: 10.1016/j.bbabio.2003.10.005. View

19.

Alboresi A, Caffarri S, Nogue F, Bassi R, Morosinotto T . In silico and biochemical analysis of Physcomitrella patens photosynthetic antenna: identification of subunits which evolved upon land adaptation. PLoS One. 2008; 3(4):e2033. PMC: 2323573. DOI: 10.1371/journal.pone.0002033. View

20.

Barber J . Photosystem II: an enzyme of global significance. Biochem Soc Trans. 2006; 34(Pt 5):619-31. DOI: 10.1042/BST0340619. View