Rose Bengal-decorated Silica Nanoparticles As Photosensitizers for Inactivation of Gram-positive Bacteria

Overview

Journal Nanotechnology

Specialty Biotechnology

Date 2010 Jan 12

PMID 20061596

Citations 28

Authors

Yanyan Guo

Snezna Rogelj

Peng Zhang

Affiliations

Soon will be listed here.

Abstract

A new type of photosensitizer, made from Rose Bengal (RB)-decorated silica (SiO(2)-NH(2)-RB) nanoparticles, was developed to inactivate gram-positive bacteria, including Methicillin-resistant Staphylococcus aureus (MRSA), with high efficiency through photodynamic action. The nanoparticles were characterized microscopically and spectroscopically to confirm their structures. The characterization of singlet oxygen generated by RB, both free and immobilized on a nanoparticle surface, was performed in the presence of anthracene-9,10-dipropionic acid. The capability of SiO(2)-NH(2)-RB nanoparticles to inactivate bacteria was tested in vitro on both gram-positive and gram-negative bacteria. The results showed that RB-decorated silica nanoparticles can inactivate MRSA and Staphylococcus epidermidis (both gram-positive) very effectively (up to eight-orders-of-magnitude reduction). Photosensitizers of such design should have good potential as antibacterial agents through a photodynamic mechanism.

Citing Articles

Comprehensive Approaches to Combatting Biofilms: From Biofilm Structure to Phage-Based Therapies.

Grygiel I, Bajrak O, Wojcicki M, Krusiec K, Jonczyk-Matysiak E, Gorski A Antibiotics (Basel). 2024; 13(11).

PMID: 39596757 PMC: 11591314. DOI: 10.3390/antibiotics13111064.

In Vitro Antimicrobial Photodynamic Therapy for () and (MRSA) Inhibition Using a Green Light Source.

Roa-Tort K, Saavedra Y, Villanueva-Martinez A, Ganem-Rondero A, Perez-Carranza L, de la Rosa-Vazquez J Pharmaceutics. 2024; 16(4).

PMID: 38675180 PMC: 11053950. DOI: 10.3390/pharmaceutics16040518.

Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells.

Uddin M, Bekmukhametova A, Antony A, Barman S, Houang J, Wu M Molecules. 2024; 29(2).

PMID: 38276623 PMC: 10820979. DOI: 10.3390/molecules29020546.

Metal nanoparticles as inhibitors of enzymes and toxins of multidrug-resistant .

Joshi A, Patil R Infect Med (Beijing). 2024; 2(4):294-307.

PMID: 38205183 PMC: 10774769. DOI: 10.1016/j.imj.2023.11.006.

Enhancing Antifungal Treatment of with Hypericin-Loaded Nanostructured Lipid Carriers in Hydrogels: Characterization, In Vitro, and In Vivo Photodynamic Evaluation.

Sato M, Oshiro-Junior J, Rodero C, Boni F, Araujo V, Bauab T Pharmaceuticals (Basel). 2023; 16(8).

PMID: 37631009 PMC: 10459110. DOI: 10.3390/ph16081094.

References

Tang W, Xu H, Kopelman R, Philbert M . Photodynamic characterization and in vitro application of methylene blue-containing nanoparticle platforms. Photochem Photobiol. 2004; 81(2):242-9. DOI: 10.1562/2004-05-24-RA-176.1. View

Wainwright M . Photodynamic antimicrobial chemotherapy (PACT). J Antimicrob Chemother. 1998; 42(1):13-28. DOI: 10.1093/jac/42.1.13. View

Bhatti M, Macrobert A, Meghji S, Henderson B, Wilson M . Effect of dosimetric and physiological factors on the lethal photosensitization of Porphyromonas gingivalis in vitro. Photochem Photobiol. 1997; 65(6):1026-31. DOI: 10.1111/j.1751-1097.1997.tb07964.x. View

Sharman W, Allen C, van Lier J . Role of activated oxygen species in photodynamic therapy. Methods Enzymol. 2000; 319:376-400. DOI: 10.1016/s0076-6879(00)19037-8. View

Embleton M, Nair S, Cookson B, Wilson M . Selective lethal photosensitization of methicillin-resistant Staphylococcus aureus using an IgG-tin (IV) chlorin e6 conjugate. J Antimicrob Chemother. 2002; 50(6):857-64. DOI: 10.1093/jac/dkf209. View

Malik Z, Hanania J, Nitzan Y . Bactericidal effects of photoactivated porphyrins--an alternative approach to antimicrobial drugs. J Photochem Photobiol B. 1990; 5(3-4):281-93. DOI: 10.1016/1011-1344(90)85044-w. View

Corbitt T, Sommer J, Chemburu S, Ogawa K, Ista L, Lopez G . Conjugated polyelectrolyte capsules: light-activated antimicrobial micro "Roach Motels". ACS Appl Mater Interfaces. 2010; 1(1):48-52. DOI: 10.1021/am800096q. View

Hamblin M, Hasan T . Photodynamic therapy: a new antimicrobial approach to infectious disease?. Photochem Photobiol Sci. 2004; 3(5):436-50. PMC: 3071049. DOI: 10.1039/b311900a. View

Yan F, Kopelman R . The embedding of meta-tetra(hydroxyphenyl)-chlorin into silica nanoparticle platforms for photodynamic therapy and their singlet oxygen production and pH-dependent optical properties. Photochem Photobiol. 2004; 78(6):587-91. DOI: 10.1562/0031-8655(2003)078<0587:teomis>2.0.co;2. View

10.

Zeina B, Greenman J, Purcell W, Das B . Killing of cutaneous microbial species by photodynamic therapy. Br J Dermatol. 2001; 144(2):274-8. DOI: 10.1046/j.1365-2133.2001.04013.x. View

11.

Wilson M, Pratten J . Lethal photosensitisation of Staphylococcus aureus. Microbios. 1994; 78(316):163-8. View

12.

Jori G, Brown S . Photosensitized inactivation of microorganisms. Photochem Photobiol Sci. 2004; 3(5):403-5. DOI: 10.1039/b311904c. View

13.

Brevet D, Gary-Bobo M, Raehm L, Richeter S, Hocine O, Amro K . Mannose-targeted mesoporous silica nanoparticles for photodynamic therapy. Chem Commun (Camb). 2009; (12):1475-7. DOI: 10.1039/b900427k. View

14.

Bertoloni G, Lauro F, Cortella G, Merchat M . Photosensitizing activity of hematoporphyrin on Staphylococcus aureus cells. Biochim Biophys Acta. 2000; 1475(2):169-74. DOI: 10.1016/s0304-4165(00)00071-4. View

15.

Dahl T, Midden W, Neckers D . Comparison of photodynamic action by Rose Bengal in gram-positive and gram-negative bacteria. Photochem Photobiol. 1988; 48(5):607-12. DOI: 10.1111/j.1751-1097.1988.tb02870.x. View

16.

Decraene V, Pratten J, Wilson M . Novel light-activated antimicrobial coatings are effective against surface-deposited Staphylococcus aureus. Curr Microbiol. 2008; 57(4):269-73. DOI: 10.1007/s00284-008-9188-7. View

17.

Rossi L, Silva P, Vono L, Fernandes A, Tada D, Baptista M . Protoporphyrin IX nanoparticle carrier: preparation, optical properties, and singlet oxygen generation. Langmuir. 2008; 24(21):12534-8. DOI: 10.1021/la800840k. View

18.

Wilson M, Yianni C . Killing of methicillin-resistant Staphylococcus aureus by low-power laser light. J Med Microbiol. 1995; 42(1):62-6. DOI: 10.1099/00222615-42-1-62. View

19.

Bhatti M, Macrobert A, Meghji S, Henderson B, Wilson M . A study of the uptake of toluidine blue O by Porphyromonas gingivalis and the mechanism of lethal photosensitization. Photochem Photobiol. 1998; 68(3):370-6. View

20.

Bezman S, Burtis P, Izod T, Thayer M . Photodynamic inactivation of E. coli by rose bengal immobilized on polystyrene beads. Photochem Photobiol. 1978; 28(3):325-9. DOI: 10.1111/j.1751-1097.1978.tb07714.x. View