Cross-resistance to UV Radiation of a Cisplatin-resistant Human Cell Line: Overexpression of Cellular Factors That Recognize UV-modified DNA

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Apr 1

PMID 2005898

Citations 19

Authors

C C Chao

S L Huang

H M Huang

S Lin-Chao

Affiliations

Soon will be listed here.

Abstract

A human cell line selected for cisplatin resistance (CPR) was irradiated with UV light and showed cross-resistance to UV light. Applying a modified chloramphenicol acetyltransferase assay, we observed that CPR cells acquired enhanced host cell reactivation of a transfected plasmid carrying UV damage. Gel mobility shift analysis indicated that two nuclear factors that recognize UV-modified DNA were overexpressed in CPR cells. In addition, factors that bind UV-modified DNA were independent from the factors that bind cisplatin-modified DNA. The significance of the identified binding factors, possibly DNA repair enzymes, is discussed.

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References

Lehmann A, Oomen A . Effect of DNA damage on the expression of the chloramphenicol acetyltransferase gene after transfection into diploid human fibroblasts. Nucleic Acids Res. 1985; 13(6):2087-95. PMC: 341137. DOI: 10.1093/nar/13.6.2087. View

Patterson M, Chu G . Evidence that xeroderma pigmentosum cells from complementation group E are deficient in a homolog of yeast photolyase. Mol Cell Biol. 1989; 9(11):5105-12. PMC: 363662. DOI: 10.1128/mcb.9.11.5105-5112.1989. View

Hamilton T, Winker M, Louie K, Batist G, Behrens B, Tsuruo T . Augmentation of adriamycin, melphalan, and cisplatin cytotoxicity in drug-resistant and -sensitive human ovarian carcinoma cell lines by buthionine sulfoximine mediated glutathione depletion. Biochem Pharmacol. 1985; 34(14):2583-6. DOI: 10.1016/0006-2952(85)90551-9. View

Rosenstein B, Chao C . Isolation of a mutant cell line derived from ICR 2A frog cells hypersensitive to the induction of non-dimer DNA damage by solar ultraviolet radiation. Somat Cell Mol Genet. 1985; 11(4):339-44. DOI: 10.1007/BF01534410. View

Kraemer K . One pyrimidine dimer inactivates expression of a transfected gene in xeroderma pigmentosum cells. Proc Natl Acad Sci U S A. 1985; 82(19):6622-6. PMC: 391262. DOI: 10.1073/pnas.82.19.6622. View

Carthew R, Chodosh L, Sharp P . An RNA polymerase II transcription factor binds to an upstream element in the adenovirus major late promoter. Cell. 1985; 43(2 Pt 1):439-48. DOI: 10.1016/0092-8674(85)90174-6. View

Klocker H, Schneider R, Burtscher H, Auer B, Hirsch-Kauffmann M, Schweiger M . Transient expression of a plasmid gene, a tool to study DNA repair in human cells: defect of DNA repair in Cockayne syndrome; one thymine cyclobutane dimer is sufficient to block transcription. Eur J Cell Biol. 1986; 39(2):346-51. View

Behrens B, Hamilton T, Masuda H, Grotzinger K, Whang-Peng J, Louie K . Characterization of a cis-diamminedichloroplatinum(II)-resistant human ovarian cancer cell line and its use in evaluation of platinum analogues. Cancer Res. 1987; 47(2):414-8. View

Chu G, Hayakawa H, Berg P . Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 1987; 15(3):1311-26. PMC: 340526. DOI: 10.1093/nar/15.3.1311. View

10.

Stark G . DNA amplification in drug resistant cells and in tumours. Cancer Surv. 1986; 5(1):1-23. View

11.

FRY D, Jackson R . Membrane transport alterations as a mechanism of resistance to anticancer agents. Cancer Surv. 1986; 5(1):47-79. View

12.

Hennighausen L, Lubon H . Interaction of protein with DNA in vitro. Methods Enzymol. 1987; 152:721-35. DOI: 10.1016/0076-6879(87)52076-6. View

13.

Wright J, Smith H, Watt F, Hancock M, Hudson D, Stark G . DNA amplification is rare in normal human cells. Proc Natl Acad Sci U S A. 1990; 87(5):1791-5. PMC: 53569. DOI: 10.1073/pnas.87.5.1791. View

14.

Chu G, Chang E . Cisplatin-resistant cells express increased levels of a factor that recognizes damaged DNA. Proc Natl Acad Sci U S A. 1990; 87(9):3324-7. PMC: 53892. DOI: 10.1073/pnas.87.9.3324. View

15.

Chao C, Lee Y, Cheng P, Lin-Chao S . Enhanced host cell reactivation of damaged plasmid DNA in HeLa cells resistant to cis-diamminedichloroplatinum(II). Cancer Res. 1991; 51(2):601-5. View

16.

Bird P, Gething M, Sambrook J . Translocation in yeast and mammalian cells: not all signal sequences are functionally equivalent. J Cell Biol. 1987; 105(6 Pt 2):2905-14. PMC: 2114712. DOI: 10.1083/jcb.105.6.2905. View

17.

Chu G, Berg P . DNA cross-linked by cisplatin: a new probe for the DNA repair defect in xeroderma pigmentosum. Mol Biol Med. 1987; 4(5):277-90. View

18.

Hospers G, Mulder N, De Vries E . Mechanisms of cellular resistance to cisplatin. Med Oncol Tumor Pharmacother. 1988; 5(3):145-51. DOI: 10.1007/BF02986437. View

19.

Eastman A, Schulte N . Enhanced DNA repair as a mechanism of resistance to cis-diamminedichloroplatinum(II). Biochemistry. 1988; 27(13):4730-4. DOI: 10.1021/bi00413a022. View

20.

Masuda H, Ozols R, Lai G, Fojo A, Rothenberg M, Hamilton T . Increased DNA repair as a mechanism of acquired resistance to cis-diamminedichloroplatinum (II) in human ovarian cancer cell lines. Cancer Res. 1988; 48(20):5713-6. View