In Vivo Fluorescence Imaging: a Personal Perspective

Overview

Journal Wiley Interdiscip Rev Nanomed Nanobiotechnol

Specialty Biotechnology

Date 2010 Jan 6

PMID 20049787

Citations 28

Authors

P Peter Ghoroghchian

Michael J Therien

Daniel A Hammer

Affiliations

Soon will be listed here.

Abstract

In vivo fluorescence imaging with near-infrared (NIR) light holds enormous potential for a wide variety of molecular diagnostic and therapeutic applications. Because of its quantitative sensitivity, inherent biological safety, and relative ease of use (i.e., with respect to cost, time, mobility, and its familiarity to a diverse population of investigators), fluorescence-based imaging techniques are being increasingly utilized in small-animal research. Moreover, there is substantial interest in the translation of novel optical techniques into the clinic, where they will prospectively aid in noninvasive and quantitative screening, disease diagnosis, and post-treatment monitoring of patients. Effective deep-tissue fluorescence imaging requires the application of exogenous NIR-emissive contrast agents. Currently, available probes fall into two major categories: organic and inorganic NIR fluorophores (NIRFs). In the studies reviewed herein, we utilized polymersomes (50 nm to 50 microm diameter polymer vesicles) for the incorporation and delivery of large numbers of highly emissive oligo (porphyrin)-based, organic NIRFs.

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References

Tyagi S, Kramer F . Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol. 1996; 14(3):303-8. DOI: 10.1038/nbt0396-303. View

Chan W, Nie S . Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science. 1998; 281(5385):2016-8. DOI: 10.1126/science.281.5385.2016. View

Susumu K, Frail P, Angiolillo P, Therien M . Conjugated chromophore arrays with unusually large hole polaron delocalization lengths. J Am Chem Soc. 2006; 128(26):8380-1. DOI: 10.1021/ja0614823. View

Weissleder R, Mahmood U . Molecular imaging. Radiology. 2001; 219(2):316-33. DOI: 10.1148/radiology.219.2.r01ma19316. View

Han M, Gao X, Su J, Nie S . Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules. Nat Biotechnol. 2001; 19(7):631-5. DOI: 10.1038/90228. View

Michalet X, Pinaud F, Bentolila L, Tsay J, Doose S, Li J . Quantum dots for live cells, in vivo imaging, and diagnostics. Science. 2005; 307(5709):538-44. PMC: 1201471. DOI: 10.1126/science.1104274. View

Benson R, Kues H . Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol. 1978; 23(1):159-63. DOI: 10.1088/0031-9155/23/1/017. View

De Grand A, Lomnes S, Lee D, Pietrzykowski M, Ohnishi S, Morgan T . Tissue-like phantoms for near-infrared fluorescence imaging system assessment and the training of surgeons. J Biomed Opt. 2006; 11(1):014007. PMC: 2486408. DOI: 10.1117/1.2170579. View

Ghoroghchian P, Frail P, Susumu K, Park T, Wu S, Uyeda H . Broad spectral domain fluorescence wavelength modulation of visible and near-infrared emissive polymersomes. J Am Chem Soc. 2005; 127(44):15388-90. DOI: 10.1021/ja055571b. View

10.

Licha K, Olbrich C . Optical imaging in drug discovery and diagnostic applications. Adv Drug Deliv Rev. 2005; 57(8):1087-108. DOI: 10.1016/j.addr.2005.01.021. View

11.

Schellenberger E, Bogdanov Jr A, Petrovsky A, Ntziachristos V, Weissleder R, Josephson L . Optical imaging of apoptosis as a biomarker of tumor response to chemotherapy. Neoplasia. 2003; 5(3):187-92. PMC: 1502408. DOI: 10.1016/S1476-5586(03)80050-7. View

12.

Rubtsov I, Susumu K, Rubtsov G, Therien M . Ultrafast singlet excited-state polarization in electronically asymmetric ethyne-bridged bis[(porphinato)zinc(II)] complexes. J Am Chem Soc. 2003; 125(9):2687-96. DOI: 10.1021/ja021157p. View

13.

Zaheer A, Lenkinski R, Mahmood A, Jones A, Cantley L, Frangioni J . In vivo near-infrared fluorescence imaging of osteoblastic activity. Nat Biotechnol. 2001; 19(12):1148-54. DOI: 10.1038/nbt1201-1148. View

14.

Tung C, Mahmood U, Bredow S, Weissleder R . In vivo imaging of proteolytic enzyme activity using a novel molecular reporter. Cancer Res. 2000; 60(17):4953-8. View

15.

Fabbri F, Franceschini M, Fantini S . Characterization of spatial and temporal variations in the optical properties of tissuelike media with diffuse reflectance imaging. Appl Opt. 2003; 42(16):3063-72. DOI: 10.1364/ao.42.003063. View

16.

Zheng G, Li H, Yang K, Blessington D, Licha K, Lund-Katz S . Tricarbocyanine cholesteryl laurates labeled LDL: new near infrared fluorescent probes (NIRFs) for monitoring tumors and gene therapy of familial hypercholesterolemia. Bioorg Med Chem Lett. 2002; 12(11):1485-8. DOI: 10.1016/s0960-894x(02)00193-2. View

17.

Wagnieres G, Cheng S, Zellweger M, Utke N, Braichotte D, Ballini J . An optical phantom with tissue-like properties in the visible for use in PDT and fluorescence spectroscopy. Phys Med Biol. 1997; 42(7):1415-26. DOI: 10.1088/0031-9155/42/7/014. View

18.

Becker A, Riefke B, Ebert B, Sukowski U, Rinneberg H, Semmler W . Macromolecular contrast agents for optical imaging of tumors: comparison of indotricarbocyanine-labeled human serum albumin and transferrin. Photochem Photobiol. 2000; 72(2):234-41. DOI: 10.1562/0031-8655(2000)072<0234:mcafoi>2.0.co;2. View

19.

Hawrysz D, Sevick-Muraca E . Developments toward diagnostic breast cancer imaging using near-infrared optical measurements and fluorescent contrast agents. Neoplasia. 2001; 2(5):388-417. PMC: 1507982. DOI: 10.1038/sj.neo.7900118. View

20.

Funovics M, Weissleder R, Tung C . Protease sensors for bioimaging. Anal Bioanal Chem. 2003; 377(6):956-63. DOI: 10.1007/s00216-003-2199-0. View