Clinical Grade Adult Stem Cell Banking

Overview

Journal Organogenesis

Specialties Anatomy & Histology
Biology
Biotechnology

Date 2010 Jan 5

PMID 20046678

Citations 41

Authors

Sreedhar Thirumala

W Scott Goebel

Erik J Woods

Affiliations

Soon will be listed here.

Abstract

There has been a great deal of scientific interest recently generated by the potential therapeutic applications of adult stem cells in human care but there are several challenges regarding quality and safety in clinical applications and a number of these challenges relate to the processing and banking of these cells ex-vivo. As the number of clinical trials and the variety of adult cells used in regenerative therapy increases, safety remains a primary concern. This has inspired many nations to formulate guidelines and standards for the quality of stem cell collection, processing, testing, banking, packaging and distribution. Clinically applicable cryopreservation and banking of adult stem cells offers unique opportunities to advance the potential uses and widespread implementation of these cells in clinical applications. Most current cryopreservation protocols include animal serum proteins and potentially toxic cryoprotectant additives (CPAs) that prevent direct use of these cells in human therapeutic applications. Long term cryopreservation of adult stem cells under good manufacturing conditions using animal product free solutions is critical to the widespread clinical implementation of ex-vivo adult stem cell therapies. Furthermore, to avoid any potential cryoprotectant related complications, reduced CPA concentrations and efficient post-thaw washing to remove CPA are also desirable. The present review focuses on the current strategies and important aspects of adult stem cell banking for clinical applications. These include current good manufacturing practices (cGMPs), animal protein free freezing solutions, cryoprotectants, freezing & thawing protocols, viability assays, packaging and distribution. The importance and benefits of banking clinical grade adult stem cells are also discussed.

Citing Articles

Autologous hGMSC-Derived iPS: A New Proposal for Tissue Regeneration.

Della Rocca Y, Diomede F, Konstantinidou F, Gatta V, Stuppia L, Benedetto U Int J Mol Sci. 2024; 25(17).

PMID: 39273117 PMC: 11395260. DOI: 10.3390/ijms25179169.

Good manufacturing practices production of human placental derived mesenchymal stem cells for therapeutic applications: focus on multiple sclerosis.

Shokati A, Naser Moghadasi A, Ghashghaei A, Sahraian M, Chahardouli B, Mousavi S Mol Biol Rep. 2024; 51(1):460.

PMID: 38551770 DOI: 10.1007/s11033-024-09372-1.

Effect of an low-energy Nd: YAG laser on periodontal ligament stem cell homing through the SDF-1/CXCR4 signaling pathway.

Wu N, Song J, Liu X, Ma X, Guo X, Liu T BMC Oral Health. 2023; 23(1):501.

PMID: 37468947 PMC: 10357748. DOI: 10.1186/s12903-023-03132-6.

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms.

Laurent A, Scaletta C, Abdel-Sayed P, Raffoul W, Hirt-Burri N, Applegate L BioTech (Basel). 2023; 12(1).

PMID: 36810442 PMC: 9944097. DOI: 10.3390/biotech12010015.

Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer.

Ding L, Razavi Bazaz S, Shrestha J, Amiri H, Mas-Hafi S, Banerjee B Micromachines (Basel). 2022; 13(9).

PMID: 36144139 PMC: 9500807. DOI: 10.3390/mi13091516.

References

Windrum P, Morris T, Drake M, Niederwieser D, Ruutu T . Variation in dimethyl sulfoxide use in stem cell transplantation: a survey of EBMT centres. Bone Marrow Transplant. 2005; 36(7):601-3. DOI: 10.1038/sj.bmt.1705100. View

de Boer F, Drager A, Pinedo H, Kessler F, van der Wall E, Jonkhoff A . Extensive early apoptosis in frozen-thawed CD34-positive stem cells decreases threshold doses for haematological recovery after autologous peripheral blood progenitor cell transplantation. Bone Marrow Transplant. 2002; 29(3):249-55. DOI: 10.1038/sj.bmt.1703357. View

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

Chan C, McCulley S, Macmillan R . Autologous fat transfer--a review of the literature with a focus on breast cancer surgery. J Plast Reconstr Aesthet Surg. 2008; 61(12):1438-48. DOI: 10.1016/j.bjps.2008.08.006. View

Benekli M, Anderson B, Wentling D, Bernstein S, Czuczman M, McCarthy P . Severe respiratory depression after dimethylsulphoxide-containing autologous stem cell infusion in a patient with AL amyloidosis. Bone Marrow Transplant. 2000; 25(12):1299-301. DOI: 10.1038/sj.bmt.1702452. View

Erices A, Conget P, Minguell J . Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000; 109(1):235-42. DOI: 10.1046/j.1365-2141.2000.01986.x. View

Liu G, Zhou H, Li Y, Li G, Cui L, Liu W . Evaluation of the viability and osteogenic differentiation of cryopreserved human adipose-derived stem cells. Cryobiology. 2008; 57(1):18-24. DOI: 10.1016/j.cryobiol.2008.04.002. View

Moscatello D, Dougherty M, Narins R, Lawrence N . Cryopreservation of human fat for soft tissue augmentation: viability requires use of cryoprotectant and controlled freezing and storage. Dermatol Surg. 2006; 31(11 Pt 2):1506-10. DOI: 10.2310/6350.2005.31235. View

Zhao J, Hao H, Thomas R, Lyman W . An efficient method for the cryopreservation of fetal human liver hematopoeitic progenitor cells. Stem Cells. 2001; 19(3):212-8. DOI: 10.1634/stemcells.19-3-212. View

10.

Kuznetsov S, FRIEDENSTEIN A, Robey P . Factors required for bone marrow stromal fibroblast colony formation in vitro. Br J Haematol. 1997; 97(3):561-70. DOI: 10.1046/j.1365-2141.1997.902904.x. View

11.

Sputtek A, Jetter S, Hummel K, Kuhnl P . Cryopreservation of peripheral blood progenitor cells: characteristics of suitable techniques. Beitr Infusionsther Transfusionsmed. 1997; 34:79-83. View

12.

Foreman M, Smith J, Publicover S . Characterisation of serum-induced intracellular Ca2+ oscillations in primary bone marrow stromal cells. J Cell Physiol. 2005; 206(3):664-71. DOI: 10.1002/jcp.20521. View

13.

Woodbury D, Schwarz E, Prockop D, Black I . Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res. 2000; 61(4):364-70. DOI: 10.1002/1097-4547(20000815)61:4<364::AID-JNR2>3.0.CO;2-C. View

14.

Noel D, Djouad F, Bouffi C, Mrugala D, Jorgensen C . Multipotent mesenchymal stromal cells and immune tolerance. Leuk Lymphoma. 2007; 48(7):1283-9. DOI: 10.1080/10428190701361869. View

15.

Beaujean F, Bourhis J, Bayle C, Jouault H, Divine M, Rieux C . Successful cryopreservation of purified autologous CD34+ cells: influence of freezing parameters on cell recovery and engraftment. Bone Marrow Transplant. 1999; 22(11):1091-6. DOI: 10.1038/sj.bmt.1701494. View

16.

Pu L, Cui X, Fink B, Cibull M, Gao D . Long-term preservation of adipose aspirates after conventional lipoplasty. Aesthet Surg J. 2009; 24(6):536-41. DOI: 10.1016/j.asj.2004.09.002. View

17.

Kawano Y, Lee C, Watanabe T, Abe T, Suzuya H, Okamoto Y . Cryopreservation of mobilized blood stem cells at a higher cell concentration without the use of a programmed freezer. Ann Hematol. 2003; 83(1):50-4. DOI: 10.1007/s00277-003-0817-8. View

18.

Matsuo A, Yamazaki Y, Takase C, Aoyagi K, Uchinuma E . Osteogenic potential of cryopreserved human bone marrow-derived mesenchymal stem cells cultured with autologous serum. J Craniofac Surg. 2008; 19(3):693-700. DOI: 10.1097/SCS.0b013e318163f2cc. View

19.

Okamoto T, Yatsuzuka N, Tanaka Y, Kan M, Yamanaka T, Sakamoto A . Growth and differentiation of periodontal ligament-derived cells in serum-free defined culture. In Vitro Cell Dev Biol Anim. 1997; 33(4):302-9. DOI: 10.1007/s11626-997-0051-0. View

20.

Lange C, Cakiroglu F, Spiess A, Cappallo-Obermann H, Dierlamm J, Zander A . Accelerated and safe expansion of human mesenchymal stromal cells in animal serum-free medium for transplantation and regenerative medicine. J Cell Physiol. 2007; 213(1):18-26. DOI: 10.1002/jcp.21081. View