Platelet Activating Factor Stimulates Arachidonic Acid Release in Differentiated Keratinocytes Via Arachidonyl Non-selective Phospholipase A2

Overview

Journal Arch Dermatol Res

Specialty Dermatology

Date 2009 Dec 31

PMID 20041255

Citations 3

Authors

Katarina Mariann Jorgensen

Hanne Solvang Felberg

Rolf K Berge

Astrid Laegreid

Berit Johansen

Affiliations

Soon will be listed here.

Abstract

Platelet activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is known to be present in excess in psoriatic skin, but its exact role is uncertain. In the present study we demonstrate for the first time the role of group VI PLA(2) in PAF-induced arachidonic acid release in highly differentiated human keratinocytes. The group IValpha PLA(2) also participates in the release, while secretory PLA(2)s play a minor role. Two anti-inflammatory synthetic fatty acids, tetradecylthioacetic acid and tetradecylselenoacetic acid, are shown to interfere with signalling events upstream of group IValpha PLA(2) activation. In summary, our major novel finding is the involvement of the arachidonyl non-selective group VI PLA(2) in PAF-induced inflammatory responses.

Citing Articles

WFDC12-overexpressing contributes to the development of atopic dermatitis via accelerating ALOX12/15 metabolism and PAF accumulation.

Li G, Gu L, Zhao F, Hu Y, Wang X, Zeng F Cell Death Dis. 2023; 14(3):185.

PMID: 36882395 PMC: 9992393. DOI: 10.1038/s41419-023-05686-3.

Platelet-activating factor induces proliferation in differentiated keratinocytes.

Feuerherm A, Jorgensen K, Sommerfelt R, Eidem L, Laegreid A, Johansen B Mol Cell Biochem. 2013; 384(1-2):83-94.

PMID: 23975504 DOI: 10.1007/s11010-013-1784-6.

Acidification in the epidermis and the role of secretory phospholipases.

Chan A, Mauro T Dermatoendocrinol. 2011; 3(2):84-90.

PMID: 21695017 PMC: 3117007. DOI: 10.4161/derm.3.2.15140.

References

Ryle C, Breitkreutz D, Stark H, Leigh I, Steinert P, Roop D . Density-dependent modulation of synthesis of keratins 1 and 10 in the human keratinocyte line HACAT and in ras-transfected tumorigenic clones. Differentiation. 1989; 40(1):42-54. DOI: 10.1111/j.1432-0436.1989.tb00812.x. View

Lin L, Lin A, Knopf J . Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid. Proc Natl Acad Sci U S A. 1992; 89(13):6147-51. PMC: 402139. DOI: 10.1073/pnas.89.13.6147. View

Sjursen W, Brekke O, Johansen B . Secretory and cytosolic phospholipase A(2)regulate the long-term cytokine-induced eicosanoid production in human keratinocytes. Cytokine. 2000; 12(8):1189-94. DOI: 10.1006/cyto.1999.0727. View

Bayerl C, Brandt H, Niemczyk M, Muller-Decker K, Gretz N . PAF-receptor in inflammatory versus non inflammatory human epidermis, cell cultures and embryonal cells. Inflamm Res. 2003; 52(7):283-6. DOI: 10.1007/s00011-003-1172-x. View

Grigoriadis G, Stewart A . Albumin inhibits platelet-activating factor (PAF)-induced responses in platelets and macrophages: implications for the biologically active form of PAF. Br J Pharmacol. 1992; 107(1):73-7. PMC: 1907628. DOI: 10.1111/j.1476-5381.1992.tb14465.x. View

Spydevold O, Bremer J . Induction of peroxisomal beta-oxidation in 7800 C1 Morris hepatoma cells in steady state by fatty acids and fatty acid analogues. Biochim Biophys Acta. 1989; 1003(1):72-9. DOI: 10.1016/0005-2760(89)90101-x. View

Anthonsen M, Andersen S, Solhaug A, Johansen B . Atypical lambda/iota PKC conveys 5-lipoxygenase/leukotriene B4-mediated cross-talk between phospholipase A2s regulating NF-kappa B activation in response to tumor necrosis factor-alpha and interleukin-1beta. J Biol Chem. 2001; 276(38):35344-51. DOI: 10.1074/jbc.M105264200. View

Ishii S, Shimizu T . Platelet-activating factor (PAF) receptor and genetically engineered PAF receptor mutant mice. Prog Lipid Res. 2000; 39(1):41-82. DOI: 10.1016/s0163-7827(99)00016-8. View

Gora S, Lambeau G, Bollinger J, Gelb M, Ninio E, Karabina S . The proinflammatory mediator Platelet Activating Factor is an effective substrate for human group X secreted phospholipase A2. Biochim Biophys Acta. 2006; 1761(9):1093-9. DOI: 10.1016/j.bbalip.2006.08.004. View

10.

Owen J, Wykle R, Samuel M, Thomas M . An improved assay for platelet-activating factor using HPLC-tandem mass spectrometry. J Lipid Res. 2004; 46(2):373-82. DOI: 10.1194/jlr.D400029-JLR200. View

11.

Candi E, Schmidt R, Melino G . The cornified envelope: a model of cell death in the skin. Nat Rev Mol Cell Biol. 2005; 6(4):328-40. DOI: 10.1038/nrm1619. View

12.

Fisher G, Talwar H, Ryder N, Voorhees J . Differential activation of human skin cells by platelet activating factor: stimulation of phosphoinositide turnover and arachidonic acid mobilization in keratinocytes but not in fibroblasts. Biochem Biophys Res Commun. 1989; 163(3):1344-50. DOI: 10.1016/0006-291x(89)91126-1. View

13.

Travers J, Murphy R, Johnson C, Pei Y, Morin S, Clay K . Identification and pharmacological characterization of platelet-activating factor and related 1-palmitoyl species in human inflammatory blistering diseases. Prostaglandins Other Lipid Mediat. 1999; 56(5-6):305-24. DOI: 10.1016/s0090-6980(98)00060-4. View

14.

PRESSMAN B . Biological applications of ionophores. Annu Rev Biochem. 1976; 45:501-30. DOI: 10.1146/annurev.bi.45.070176.002441. View

15.

Singer A, Ghomashchi F, Le Calvez C, Bollinger J, Bezzine S, Rouault M . Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2. J Biol Chem. 2002; 277(50):48535-49. DOI: 10.1074/jbc.M205855200. View

16.

Murakami M, Koduri R, Enomoto A, Shimbara S, Seki M, Yoshihara K . Distinct arachidonate-releasing functions of mammalian secreted phospholipase A2s in human embryonic kidney 293 and rat mastocytoma RBL-2H3 cells through heparan sulfate shuttling and external plasma membrane mechanisms. J Biol Chem. 2000; 276(13):10083-96. DOI: 10.1074/jbc.M007877200. View

17.

OFlaherty J, Surles J, Redman J, Jacobson D, Piantadosi C, Wykle R . Binding and metabolism of platelet-activating factor by human neutrophils. J Clin Invest. 1986; 78(2):381-8. PMC: 423558. DOI: 10.1172/JCI112588. View

18.

Yokota Y, Hanasaki K, Ono T, Nakazato H, Kobayashi T, Arita H . Suppression of murine endotoxic shock by sPLA2 inhibitor, indoxam, through group IIA sPLA2-independent mechanisms. Biochim Biophys Acta. 1999; 1438(2):213-22. DOI: 10.1016/s1388-1981(99)00053-0. View

19.

Boukamp P, Petrussevska R, Breitkreutz D, Hornung J, Markham A, Fusenig N . Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol. 1988; 106(3):761-71. PMC: 2115116. DOI: 10.1083/jcb.106.3.761. View

20.

Eckert R, Lee K . S100A7 (Psoriasin): a story of mice and men. J Invest Dermatol. 2006; 126(7):1442-4. DOI: 10.1038/sj.jid.5700265. View