» Articles » PMID: 20033450

Epigenetic Marks in the Mature Pollen of Quercus Suber L. (Fagaceae)

Overview
Specialty Biology
Date 2009 Dec 25
PMID 20033450
Citations 14
Authors
Affiliations
Soon will be listed here.
Abstract

We have analysed the distribution of epigenetic marks for histone modifications at lysine residues H3 and H4, and DNA methylation, in the nuclei of mature pollen cells of the Angiosperm tree Quercus suber; a monoecious wind pollinated species with a protandrous system, and a long post-pollination period. The ultrasonic treatment developed for the isolation of pollen nuclei proved to be a fast and reliable method, preventing the interference of cell wall autofluorescence in the in situ immunolabelling assays. In contrast with previous studies on herbaceous species with short progamic phases, our results are consistent with a high level of silent (5-mC and H3K9me2) epigenetic marks on chromatin of the generative nucleus, and the prevalence of active marks (H3K9me3 and H4Kac) in the vegetative nucleus. The findings are discussed in terms of the pollination/fertilization timing strategy adopted by this plant species.

Citing Articles

Exploring the crop epigenome: a comparison of DNA methylation profiling techniques.

Agius D, Kapazoglou A, Avramidou E, Baranek M, Carneros E, Caro E Front Plant Sci. 2023; 14:1181039.

PMID: 37389288 PMC: 10306282. DOI: 10.3389/fpls.2023.1181039.


Epigenetic variation: A major player in facilitating plant fitness under changing environmental conditions.

Rajpal V, Rathore P, Mehta S, Wadhwa N, Yadav P, Berry E Front Cell Dev Biol. 2022; 10:1020958.

PMID: 36340045 PMC: 9628676. DOI: 10.3389/fcell.2022.1020958.


Genome-wide identification, phylogeny, and gene duplication of the epigenetic regulators in Fagaceae.

Alves S, Braga A, Parreira D, Alhinho A, Silva H, Ramos M Physiol Plant. 2022; 174(5):e13788.

PMID: 36169620 PMC: 9828519. DOI: 10.1111/ppl.13788.


Multiomics Molecular Research into the Recalcitrant and Orphan Tree Species: Why, What for, and How.

Maldonado-Alconada A, Castillejo M, Rey M, Labella-Ortega M, Tienda-Parrilla M, Hernandez-Lao T Int J Mol Sci. 2022; 23(17).

PMID: 36077370 PMC: 9456323. DOI: 10.3390/ijms23179980.


Heat Stress in Somatic Embryogenesis Induction: Effect in DNA Methylation and Differential Expression of Stress-Related Genes.

Pereira C, Castander-Olarieta A, Sales E, Montalban I, Canhoto J, Moncalean P Plants (Basel). 2021; 10(11).

PMID: 34834696 PMC: 8622292. DOI: 10.3390/plants10112333.


References
1.
Okada T, Singh M, Bhalla P . Transcriptome profiling of Lilium longiflorum generative cells by cDNA microarray. Plant Cell Rep. 2007; 26(7):1045-52. DOI: 10.1007/s00299-006-0300-9. View

2.
Janousek B, Matsunaga S, Kejnovsky E, Ziuvova J, Vyskot B . DNA methylation analysis of a male reproductive organ specific gene (MROS1) during pollen development. Genome. 2002; 45(5):930-8. DOI: 10.1139/g02-052. View

3.
Richards E, Elgin S . Epigenetic codes for heterochromatin formation and silencing: rounding up the usual suspects. Cell. 2002; 108(4):489-500. DOI: 10.1016/s0092-8674(02)00644-x. View

4.
Pan G, Zhou Y, Fowke L, Wang H . An efficient method for flow cytometric analysis of pollen and detection of 2n nuclei in Brassica napus pollen. Plant Cell Rep. 2004; 23(4):196-202. DOI: 10.1007/s00299-004-0830-y. View

5.
Jasencakova Z, Meister A, Walter J, Turner B, Schubert I . Histone H4 acetylation of euchromatin and heterochromatin is cell cycle dependent and correlated with replication rather than with transcription. Plant Cell. 2000; 12(11):2087-100. PMC: 150160. DOI: 10.1105/tpc.12.11.2087. View