Formation of Secretory Granules by Chromogranins

Overview

Journal Med Mol Morphol

Publisher Springer

Specialties Cell Biology
Molecular Biology
Pathology

Date 2009 Dec 25

PMID 20033364

Authors

Chie Inomoto

Robert Yoshiyuki Osamura

Affiliations

Soon will be listed here.

Abstract

This review article covers the molecular mechanisms of secretory granule formation by chromogranin transfection. Recently, a few investigators have reported that the transfection of chromogranin A and B produces the structures of secretory granules. We used the GFP-chromogranin A transfection method to nonendocrine cells, COS-7 cells, which are not equipped with secretory granules. Despite the absence of endogenous secretory granules in nontransfected COS-7 cells, COS-7 cells transfected with chromogranin A contained granule-like structures in electron micrographs. The granules were composed of an outer limiting membrane with core structures that were interpreted as secretory granules. Human chromogranin A (CgA) labeled with 5-nm gold particles was present in several dense-core granules in our previous electron microscopy study. This review depicts the role of chromogranin A in the formation of secretory granules. It emphasizes the application of recently developed new technologies and the genesis of secretory granules.

References

Urbe S, Page L, Tooze S . Homotypic fusion of immature secretory granules during maturation in a cell-free assay. J Cell Biol. 1998; 143(7):1831-44. PMC: 2175232. DOI: 10.1083/jcb.143.7.1831. View

Tvarusko W, Bentele M, Misteli T, Rudolf R, Kaether C, Spector D . Time-resolved analysis and visualization of dynamic processes in living cells. Proc Natl Acad Sci U S A. 1999; 96(14):7950-5. PMC: 22168. DOI: 10.1073/pnas.96.14.7950. View

Simon J, Aunis D . Biochemistry of the chromogranin A protein family. Biochem J. 1989; 262(1):1-13. PMC: 1133222. DOI: 10.1042/bj2620001. View

Huh Y, Jeon S, Yoo S . Chromogranin B-induced secretory granule biogenesis: comparison with the similar role of chromogranin A. J Biol Chem. 2003; 278(42):40581-9. DOI: 10.1074/jbc.M304942200. View

Eriksson B, Arnberg H, Oberg K, Hellman U, Lundqvist G, Wernstedt C . A polyclonal antiserum against chromogranin A and B--a new sensitive marker for neuroendocrine tumours. Acta Endocrinol (Copenh). 1990; 122(2):145-55. DOI: 10.1530/acta.0.1220145. View

Yoo S . pH-dependent association of chromogranin A with secretory vesicle membrane and a putative membrane binding region of chromogranin A. Biochemistry. 1993; 32(32):8213-9. DOI: 10.1021/bi00083a023. View

Fischer-Colbrie R, Lassmann H, Hagn C, Winkler H . Immunological studies on the distribution of chromogranin A and B in endocrine and nervous tissues. Neuroscience. 1985; 16(3):547-55. DOI: 10.1016/0306-4522(85)90191-5. View

Kim T, Tao-Cheng J, Eiden L, Loh Y . Chromogranin A, an "on/off" switch controlling dense-core secretory granule biogenesis. Cell. 2001; 106(4):499-509. DOI: 10.1016/s0092-8674(01)00459-7. View

Linnoila R, Mulshine J, Steinberg S, Funa K, Matthews M, Cotelingam J . Neuroendocrine differentiation in endocrine and nonendocrine lung carcinomas. Am J Clin Pathol. 1988; 90(6):641-52. DOI: 10.1093/ajcp/90.6.641. View

10.

Tooze S, Martens G, Huttner W . Secretory granule biogenesis: rafting to the SNARE. Trends Cell Biol. 2001; 11(3):116-22. DOI: 10.1016/s0962-8924(00)01907-3. View

11.

Tooze S, Flatmark T, Tooze J, Huttner W . Characterization of the immature secretory granule, an intermediate in granule biogenesis. J Cell Biol. 1991; 115(6):1491-503. PMC: 2289211. DOI: 10.1083/jcb.115.6.1491. View

12.

Yoo S . pH-dependent interaction of chromogranin A with integral membrane proteins of secretory vesicle including 260-kDa protein reactive to inositol 1,4,5-triphosphate receptor antibody. J Biol Chem. 1994; 269(16):12001-6. View

13.

Kaether C, Gerdes H . Visualization of protein transport along the secretory pathway using green fluorescent protein. FEBS Lett. 1995; 369(2-3):267-71. DOI: 10.1016/0014-5793(95)00765-2. View

14.

Lloyd R, Iacangelo A, Eiden L, Cano M, Jin L, Grimes M . Chromogranin A and B messenger ribonucleic acids in pituitary and other normal and neoplastic human endocrine tissues. Lab Invest. 1989; 60(4):548-56. View

15.

Rustom A, Bajohrs M, Kaether C, Keller P, Toomre D, Corbeil D . Selective delivery of secretory cargo in Golgi-derived carriers of nonepithelial cells. Traffic. 2002; 3(4):279-88. DOI: 10.1034/j.1600-0854.2002.030405.x. View

16.

Lloyd R, Cano M, Rosa P, Hille A, Huttner W . Distribution of chromogranin A and secretogranin I (chromogranin B) in neuroendocrine cells and tumors. Am J Pathol. 1988; 130(2):296-304. PMC: 1880525. View

17.

Wacker I, Kaether C, Kromer A, Migala A, Almers W, Gerdes H . Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein. J Cell Sci. 1997; 110 ( Pt 13):1453-63. DOI: 10.1242/jcs.110.13.1453. View

18.

Taupenot L . Analysis of regulated secretion using PC12 cells. Curr Protoc Cell Biol. 2008; Chapter 15:Unit 15.12. DOI: 10.1002/0471143030.cb1512s36. View

19.

Stilling G, Bayliss J, Jin L, Zhang H, Lloyd R . Chromogranin A transcription and gene expression in Folliculostellate (TtT/GF) cells inhibit cell growth. Endocr Pathol. 2005; 16(3):173-86. DOI: 10.1385/ep:16:3:173. View

20.

Inomoto C, Umemura S, Egashira N, Minematsu T, Takekoshi S, Itoh Y . Granulogenesis in non-neuroendocrine COS-7 cells induced by EGFP-tagged chromogranin A gene transfection: identical and distinct distribution of CgA and EGFP. J Histochem Cytochem. 2007; 55(5):487-93. DOI: 10.1369/jhc.6A7110.2007. View