Airway Smooth Muscle Proliferation and Survival is Not Modulated by Mast Cells

Overview

Journal Clin Exp Allergy

Specialty Allergy & Immunology

Date 2009 Dec 25

PMID 20030664

Citations 15

Authors

D Kaur

F Hollins

R Saunders

L Woodman

A Sutcliffe

G Cruse

P Bradding

C Brightling

Affiliations

Soon will be listed here.

Abstract

Background: Airway smooth muscle (ASM) hyperplasia and mast cell localization within the ASM bundle are important features of asthma. The cause of this increased ASM mass is uncertain and whether it is a consequence of ASM-mast cell interactions is unknown.

Objective: We sought to investigate ASM proliferation and survival in asthma and the effects of co-culture with mast cells.

Methods: Primary ASM cultures were derived from 11 subjects with asthma and 12 non-asthmatic controls. ASM cells were cultured for up to 10 days in the presence or absence of serum either alone or in co-culture with the human mast cell line-1, unstimulated human lung mast cells (HLMC) or IgE/anti-IgE-activated HLMC. Proliferation was assessed by cell counts, CFSE assay and thymidine incorporation. Apoptosis and necrosis were analysed by Annexin V/propidium iodide staining using flow cytometry and by assessment of nuclear morphology using immunofluorescence. Mast cell activation was confirmed by the measurement of histamine release.

Results: Using a number of techniques, we found that ASM proliferation and survival was not significantly different between cells derived from subjects with or without asthma. Co-culture with mast cells did not affect the rate of proliferation or survival of ASM cells.

Conclusion: Our findings do not support a role for increased airway smooth proliferation and survival as the major mechanism driving ASM hyperplasia in asthma.

Citing Articles

Asthmatic Bronchial Matrices Determine the Gene Expression and Behavior of Smooth Muscle Cells in a 3D Culture Model.

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Orosomucoid-like 3 (ORMDL3) upregulates airway smooth muscle proliferation, contraction, and Ca oscillations in asthma.

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Mast cells in airway diseases and interstitial lung disease.

Cruse G, Bradding P Eur J Pharmacol. 2015; 778:125-38.

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CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET.

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PMID: 25713319 PMC: 4421784. DOI: 10.1152/ajplung.00021.2015.

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