High-resolution Protein Complexes from Integrating Genomic Information with Molecular Simulation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Dec 19

PMID 20018738

Citations 73

Authors

Alexander Schug

Martin Weigt

Jose N Onuchic

Terence Hwa

Hendrik Szurmant

Affiliations

Soon will be listed here.

Abstract

Bacteria use two-component signal transduction systems (TCS) extensively to sense and react to external stimuli. In these, a membrane-bound sensor histidine kinase (SK) autophosphorylates in response to an environmental stimulus and transfers the phosphoryl group to a transcription factor/response regulator (RR) that mediates the cellular response. The complex between these two proteins is ruled by transient interactions, which provides a challenge to experimental structure determination techniques. The functional and structural homolog of an SK/RR pair Spo0B/Spo0F, however, has been structurally resolved. Here, we describe a method capable of generating structural models of such transient protein complexes. By using existing structures of the individual proteins, our method combines bioinformatically derived contact residue information with molecular dynamics simulations. We find crystal resolution accuracy with existing crystallographic data when reconstituting the known system Spo0B/Spo0F. Using this approach, we introduce a complex structure of TM0853/TM0468 as an exemplary SK/RR TCS, consistent with all experimentally available data.

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